LOCUS       sequence-28849-e         757 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Hosokawa N, Sasaki T, Iemura S, Natsume T, Hara T, Mizushima N
  TITLE     Atg101, a novel mammalian autophagy protein interacting with Atg13.
  JOURNAL   Autophagy. 2009 Oct . 5(7):973-9.
  PUBMED    19597335
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy. 
            2009 Oct . 5(7):973-9."
COMMENT     Sequence Label: pCI-neo-HA-hAtg13
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          165..297
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     280..299
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        342..360
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             384..473
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 gagctcgttt agtgaccgtc agatcactag aagctttatt gcggtagttt atcacagtta
       61 aattgctaac gcagtcagtg cttctgacac aacagtctcg aacttaagct gcagtgactc
      121 tcttaaggta gccttgcaga agttggtcgt gaggcactgg gcaggtaagt atcaaggtta
      181 caagacaggt ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc
      241 gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg
      301 tccactccca gttcaattac agctcttaag gctagagtac ttaatacgac tcactatagg
      361 ctagcctcga gagaatgatc ttttacccat acgatgttcc tgactatgcg ggctatccct
      421 atgacgtccc ggactatgca ggatcctatc catatgacgt tccagattac gctgctcagt
      481 gcactcgaga attcacgcgt ggtacctcta gaatggaaac tgatctcaat tcccaggaca
      541 gaaaggacct ggacaagttt attaaatttt ttgccctcaa gactgtccaa gtgattgtcc
      601 aggctcggct tggtgaaaag atttgcactc gttcatcatc ttctccaacg ggttcagatt
      661 ggttcaactt agcaatcaaa gacatcccag aggttacaca tgaagcaaag aangcactgg
      721 caggacagct gcctgcagtc gggaggtcca tgtgtgt
//
