LOCUS       sequence-288647-        6847 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Insect Expression of DmMe31B-K108A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Tritschler F, Braun JE, Eulalio A, Truffault V, Izaurralde E, 
            Weichenrieder O
  TITLE     Structural basis for the mutually exclusive anchoring of P body 
            components EDC3 and Tral to the DEAD box protein DDX6/Me31B.
  JOURNAL   Mol Cell. 2009 Mar 13. 33(5):661-8.
  PUBMED    19285948
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Mar 13. 33(5):661-8."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmMe31B-K108A_E
FEATURES             Location/Qualifiers
     source          1..6847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     161..180
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(399..503)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     571..589
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        653..3165
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             3185..3250
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             3275..3301
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4729..4770
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4780..4797
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4820..4837)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4870..5004
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4894..4913)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4948..4967
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5626..6214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5706..5725)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6385..6847,1..329,330..398))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
       61 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
      121 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
      181 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
      241 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
      301 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
      361 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
      421 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
      481 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
      541 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
      601 caaggaatta attctatatt ctaaaaacac aaatgatact tctaaaaaaa atcatgaatg
      661 gcatcaactc tgaatcaaat ctttgcagat gcacctactt ctcatttcca ctgtcacatc
      721 atttttccag atctcgctgc ctgttatgtg gcccacaaac caagacacgt tttatggcca
      781 ttaaagctgg ctgatcgtcg ccaaacacca aatacatatc aatatgtaca ttcgagaaag
      841 aagcgatcaa agaagcgtct tcgggcgagt aggagaatgc ggaggagaag gagaacgagc
      901 tgatctagta tctctccaca atccaatgcc aactgaccaa ctggccatat tcggagcaat
      961 ttgaagccaa tttccatcgc ctggcgatcg ctccattctt ggctatatgt ttttcaccgt
     1021 tcccggggcc attttcaaag actcgtcggt aagataagat tgtgtcactc gctgtctctc
     1081 ttcatttgtc gaagaatgct gaggaatttc gcgatgacgt cggcgagtat tttgaagaat
     1141 gagaataatt tgtatttata cgaaaatcag ttagtggaat tttctacaaa aacatgttat
     1201 ctatagataa ttttgttgca aaatatgttg actatgacaa agattgtatg tatatacctt
     1261 taatgtattc tcattttctt atgtatttat aatggcaatg atgatactga tgatatttta
     1321 agatgatgcc agaccacagg ctgatttctg cgtcttttgc cgaacgcagt gcatgtgcgg
     1381 ttgttgtttt ttggaatagt ttcaattttc ggactgtccg ctttgatttc agtttcttgg
     1441 cttattcaaa aagcaaagta aagccaaaaa agcgagatgg caataccaaa tgcggcaaaa
     1501 cggtagtgga aggaaagggg tgcggggcag cggaaggaag ggtggggcgg ggcgtggcgg
     1561 ggtctgtggc tgggcgcgac gtcaccgacg ttggagccac tcctttgacc atgtgtgcgt
     1621 gtgtgtatta ttcgtgtctc gccactcgcc ggttgttttt ttctttttat ctcgctctct
     1681 ctagcgccat ctcgtacgca tgctcaacgc accgcatgtt gccgtgtcct ttatgcgtca
     1741 ttttggctcg aaataggcaa ttatttaaac aaagattagt caacgaaaac gctaaaataa
     1801 ataagtctac aatatggtta cttattgcca tgtgtgtgca gccaacgata gcaacaaaag
     1861 caacaacaca gtggctttcc ctctttcact ttttgtttgc aagcgcgtgc gagcaagacg
     1921 gcacgaccgg caaacgcaat tacgctgaca aagagcagac gaagttttgg ccgaaaaaca
     1981 tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc gatatttaat attgtttatg
     2041 aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa caaattattt gaaagagaat
     2101 taggaatcgg acagcttatc gttacgggct aacagcacac cgagacgaaa tagcttacct
     2161 gacgtcacag cctctggaag aactgccgcc aagcagagag agagagaaaa agagggagag
     2221 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctcc tgtttgcgca
     2281 aacgcataga ctgcactgag aaaatcgatt acctattttt tatgaatgaa tatttgcact
     2341 attactattc aaaactatta agatagcaat cacattcaat agccaaatac tataccacct
     2401 gagcgatgca acgaaatgat caatttgagc aaaaatgctg catatttagg acggcatcat
     2461 tatagaaatg cttcttgctg tgtacttttc tctcgtctgg cagctgtttc gccgttattg
     2521 ttaaaaccgg cttaagttag gtgtgttttc tacgactagt gatgccccta ctagaagatg
     2581 tgtgttgcac aaatgtccct gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2641 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2701 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2761 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2821 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2881 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2941 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     3001 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     3061 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     3121 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     3181 caacatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
     3241 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
     3301 tcctccaagc ttgatgatga ctgaaaagtt aaattctggg cacactaatt taacaagcaa
     3361 ggggattata aatgatcttc aaattgctgg aaacacaagt gacgatatgg gctggaaatc
     3421 aaagctgaag ctgccgccaa aggacaaccg attcaaaaca actgatgtga ccgatacgcg
     3481 aggcaatgaa ttcgaggagt tttgccttaa aagagaactg cttatgggta tattcgagaa
     3541 gggatgggag cgcccttcgc caattcagga agcagctatt cctatagcat taagtggaaa
     3601 agatgtgctg gctcgggcca agaacggcac aggcgcaaca ggagcctact gcatcccagt
     3661 tttagaacaa atcgacccaa ccaaggacta cattcaggca cttgtgatgg tccccacccg
     3721 agagttggcg ctacaaacct cacagatatg tattgagctg gcgaaacatc ttgatatccg
     3781 ggtaatggtc acaacgggag gtaccattct aaaggatgac attcttcgta tttatcaaaa
     3841 agtacaatta attattgcca ctcccggacg aatattagat ctgatggata agaaagttgc
     3901 cgatatgtcg cattgcagaa tattagtttt ggatgaggcc gataagctat tgtcgcttga
     3961 ttttcaaggc atgctggatc atgttatact aaagttacca aaggatccac agatcttgct
     4021 attttccgca acatttccac taacagtcaa gaatttcatg gagaagcatt tacgcgagcc
     4081 ttacgagatc aacctgatgg aggagctaac actgaagggt gtcactcagt actatgcatt
     4141 cgtacaggag cgccagaaag tacactgctt gaatacgctc ttttcgaagc tgcagatcaa
     4201 tcagtctatc atattctgca attccacgca acgtgtcgag ctattggcca aaaagatcac
     4261 agagcttgga tattgctgtt actatataca cgctaagatg gcccaggcac acagaaatcg
     4321 agtattccac gatttccgcc aaggactctg tcgcaatctg gtgtgctccg acctgttcac
     4381 ccgtggtatc gacgtgcagg ccgtgaatgt agtaatcaat ttcgatttcc cacgaatggc
     4441 agagacatac ttgcatcgaa ttggtcgctc aggacgtttc ggtcatttgg gtattgctat
     4501 taatctgata acctacgagg atcggtttga tctgcatcgg attgaaaaag aacttggaac
     4561 cgaaataaag cctattccga aggtcataga ccccgccctt tatgtcgcaa acgttggcgc
     4621 atccgttggc gatacttgca acaacagtga tctgaataat tctgcgaacg aggagggcaa
     4681 cgttagcaaa taagcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc
     4741 cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga
     4801 gtttaaaccc gctgatcagc ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc
     4861 tcgatcgagg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     4921 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     4981 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     5041 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     5101 gattatgatc agtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     5161 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     5221 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
     5281 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
     5341 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
     5401 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
     5461 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
     5521 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
     5581 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5641 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5701 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5761 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5821 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5881 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5941 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6001 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6061 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6121 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6181 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6241 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6301 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6361 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6421 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6481 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6541 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6601 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6661 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     6721 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6781 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6841 taagttg
//
