LOCUS       sequence-288742-       10545 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian Expression of HsTNRC6A-delMII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 10545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6A-delMII_F
FEATURES             Location/Qualifiers
     source          1..10545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1179..1205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    5291..5302
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6264..6283)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6318..6337
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6556..7011
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6643..6662)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6853..6874
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7128..7485
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7128..7319
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7151..7171)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7336..7471
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7536..8330
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7590..7609)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8200..8219
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8394..8442
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8483..8502)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8602..8624
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8662..8680)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8748..8852
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8853..8921,8922..9713)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9071..9090)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9884..10472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10373..10392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg agatggctta cccatacgat gttccagatt
     1201 acgctatgga tgctgattct gcctccagtt ctgaatcaga gagaaacatc actatcatgg
     1261 cttcagggaa cacaggtggt gaaaaagatg gccttcggaa tagcactgga cttggttccc
     1321 aaaacaagtt tgtagttggt agcagcagca ataatgtggg ccatggaagt agtactgggc
     1381 catggggttt ttcccatgga gccataataa gcacatgtca ggtctctgtg gatgctcctg
     1441 aaagcaaatc tgaaagtagc aacaatagaa tgaatgcttg gggcactgta agttcttcat
     1501 caaatggagg gttaaatcca agcactttga attcagctag caaccatggt gcctggccag
     1561 tattagagaa caatggactt gccctaaaag ggcctgtagg gagtggtagt tctggcatta
     1621 atattcagtg cagtactata ggccagatgc ctaacaatca gagtattaac tctaaagtga
     1681 gtggtggttc tacccatggt acctggggaa gccttcagga aacttgtgaa tctgaagtaa
     1741 gtggtacaca gaaggtttca ttcagtggtc aacctcaaaa tattaccact gaaatgactg
     1801 gaccaaataa cactactaac tttatgacct ctagtttacc aaactccggt tcagtgcaga
     1861 ataatgagct gcctagtagt aacacagggg cctggcgtgt gagcacaatg aatcatcctc
     1921 agatgcaggc tccatcaggt atgaatggca cttccctttc tcaccttagc aatggagagt
     1981 caaaaagtgg aggctcttat ggtactacat ggggtgccta tggttctaat tactctggag
     2041 acaaatgttc aggccctaat ggccaagcta atggtgacac tgtgaatgca actctaatgc
     2101 agcctggcgt aaatggtcct atgggcacta actttcaagt taacacaaac aaaggaggtg
     2161 gtgtgtggga atctggtgca gcaaactccc agagtacatc atggggaagt ggaaatggcg
     2221 caaattctgg aggaagtcga agaggatggg gaacccctgc acaaaacact ggcactaatt
     2281 tacccagcgt tgagtggaac aaactgccta gcaatcagca ttccaatgat agtgcaaatg
     2341 gcaatggtaa gacgtttaca aatggatgga aatctactga ggaagaggat cagggttctg
     2401 ccacatctca gacaaatgag caaagcagtg tgtgggccaa aacaggaggt acagtggaga
     2461 gcgatggtag tacagaaagc actggacgcc ttgaggaaaa aggaactggg gaaagtcaga
     2521 gtagagacag aagaaaaatt gatcagcaca cattactcca aagcattgta aacagaactg
     2581 acttagatcc acgtgtcctg tccaactctg gttggggaca gactcctatt aagcagaata
     2641 ctgcctggga tacagaaaca tcacctagag gggaacgaaa gactgacaat gggacagagg
     2701 cctggggaag ctctgcaaca cagactttta actcaggggc atgtatagat aagactagcc
     2761 ctaatggtaa tgatacctca tctgtatcag ggtggggcga tcccaaacct gctctgaggt
     2821 ggggagattc caaaggctca aactgccagg gggggtggga agatgattct gctgctacag
     2881 gaatggtcaa gagcaatcag tgggggaatt gcaaagagga gaaggctgca tggaatgact
     2941 cgcaaaagaa taaacaggga tggggtgatg gacaaaaatc aagccaaggg tggtctgttt
     3001 ctgccagtga taactgggga gaaacttcaa ggaataacca ttggggtgag gccaataaga
     3061 aatccagctc aggaggtagt gacagtgaca ggtccgtttc cggttggaac gaacttggta
     3121 aaactagttc tttcacttgg ggaaacaaca taaatccaaa taattcatca ggatgggatg
     3181 aatcttctaa acctactcct tcccagggat ggggagaccc tccaaagtct aatcagtctc
     3241 taggttgggg agattcgtca aagccagtca gctctccaga ctggaacaag caacaagaca
     3301 ttgttggatc ttggggaatc ccaccagcta caggcaaacc tcctggtaca ggctggctgg
     3361 ggggacctat accagcccca gcaaaagaag aagtcccaaa tggcaacagc cgttcagacc
     3421 agcaagcaca ggtacatcag ctgctaacgc ctgcaagtgc catctcaaac aaagaggcaa
     3481 gcagtggctc tggctggggt gagccctggg gggagccttc tactccagcc acaactgtgg
     3541 ataatggtac ttcagcatgg ggtaagccca tagacagtgg tcccagctgg ggggaaccca
     3601 ttgctgcggc atccagcaca tccacgtggg gctccagctc tgttggtcca caagcattaa
     3661 gcaaatctgg gccaaaatct atgcaagatg gctggtgtgg tgatgatatg ccattgcctg
     3721 gaaatcgccc cactggctgg gaagaggaag aggatgtgga gattggaatg tggaatagta
     3781 attcatctca agagcttaac tcatctttaa attggccacc atatacaaag aaaatgtcat
     3841 cgaagggtct gagtggcaaa aaaaggagaa gggaaagggg aatgatgaaa ggtggaaaca
     3901 aacaagaaga agcgtggata aatccatttg ttaaacagtt ttcaaacatc agtttttcga
     3961 gagactcacc agaggaaaat gtacaaagca ataagatgga cctttctgga ggaatgttac
     4021 aagacaaacg aatggagata gataaacata gcctaaatat tggtgattac aatcgaacgg
     4081 tcgggaaagg ccctggttct cggcctcaga tttccaaaga gtcttccatg gagcgcaatc
     4141 cttattttga taaggatggc attgtagcag atgaatccca aaacatgcag tttatgtcca
     4201 gtcaaagcat gaagcttccc ccttcaaata gtgcactacc taaccaggcc cttggctcca
     4261 tagcagggct gggtatgcaa aacttgaatt ctgttagaca gaatggcaat cccagtatgt
     4321 ttggtgttgg aaacacagca gcacaacccc ggggcatgca gcagcctcca gcacaacctc
     4381 ttagttcatc tcagcctaat ctccgtgctc aagtgcctcc tccattactc tcccctcagg
     4441 ttccagtttc attgctgaag tatgcaccaa acaacggtgg cctgaatcca ctctttggcc
     4501 ctcaacaggt agccatgctg aaccagctat cccagctaaa ccagctttct cagatctccc
     4561 agttacagcg attgttagcg cagcagcaaa gggcgcagag tcagagaagc gtgccttctg
     4621 ggaaccggcc gcagcaagac cagcagggtc gacctcttag tgtgcagcag caaatgatgc
     4681 aacaatctcg tcaacttgat ccaaacctgt tggtgaagca gcagactcca ccatctcagc
     4741 agcagccact ccatcagcca gccatgaagt ctttccttga caatgtcatg ccccacacta
     4801 cacctgagct gcaaaaaggg ccatcaccaa taaatgcttt cagcaacttc cctataggct
     4861 tgaactcaaa cttgaatgta aatatggata tgaacagtat taaagagcca cagtcaagac
     4921 taaggaagtg gacgacagtg gacagcattt ctgtgaacac atctttggat caaaactcca
     4981 gcaaacatgg tgctatttca agtggtttca ggctggaaga gtctccattt gttccctatg
     5041 actttatgaa cagcagtact tcaccagcca gtcctccagg ttcaatagga gatggctggc
     5101 cacgtgccaa atcgcctaac ggctctagca gtgttaattg gccaccagaa tttcgtcctg
     5161 gtgagccatg gaaaggttat ccaaacattg accctgaaac tgacccttac gtcactcctg
     5221 gcagtgtcat aaacaatctt tcaattaata ctgtgcggga agttgaccac ctcagggaca
     5281 ggaacagtgg gtcatcctca tccttgaaca ccacgctgcc ttcaactagt gcctggtcat
     5341 ccattcgtgc ctccaactac aacgttcccc tcagcagtac agcacaaagc acttcagcca
     5401 gaaatagtga ttccaaattg acatggtctc ctggttcagt tacaaacacc tctctggctc
     5461 atgagctgtg gaaggtccct ttgccaccta aaaacatcac tgctccgtcc cgcccacctc
     5521 cgggactgac tggtcagaag ccacccttgt ctacgtggga taattctccc cttcgtatag
     5581 gtggaggatg gggaaattct gacgccagat ataccccagg ttccagctgg ggtgagagca
     5641 gctcagggag aataacaaat tggcttgttc taaaaaacct tacacctcag atcgatggct
     5701 caactctgcg cactctgtgc atgcagcacg gcccgctgat cacattccac ctgaacctcc
     5761 ctcacggaaa tgctctggtc cgctacagtt caaaagaaga ggtagtggag gcacaaaagt
     5821 ctctgcacat gtgtgtactg gggaacacta ctattcttgc tgagtttgcc agtgaagagg
     5881 agatcagtcg tttctttgca caaagccagt ctctgacccc ttctcccggc tggcagtctc
     5941 tcgggtccag ccagagccgg ctgggctccc tcgactgttc ccactcattc tccagccgga
     6001 ccgatctcaa tcactggaat ggtgctgggc tgtcgggaac taactgtgga gaccttcacg
     6061 gcacttcact ctgggggacc ccgcattatt ccacaagcct gtggggtccc ccaagcagca
     6121 gcgacccccg aggaattagc agcccatctc ccattaacgc ttttctttct gttgaccacc
     6181 tgggtggggg tggagagtcc atgtaagcgg ccgcttccct ttagtgaggg ttaatgcttc
     6241 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     6301 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     6361 gcaataaaca agtaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     6421 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatccgat aaggatcgat
     6481 ccgggctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     6541 ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6601 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6661 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6721 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6781 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6841 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6901 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6961 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg
     7021 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgcggat ctgcgcagca
     7081 ccatggcctg aaataacctc tgaaagagga acttggttag gtaccttctg aggcggaaag
     7141 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     7201 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7261 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7321 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7381 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     7441 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tgattcttct
     7501 gacacaacag tctcgaactt aaggctagag ccaccatgat tgaacaagat ggattgcacg
     7561 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     7621 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     7681 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     7741 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     7801 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     7861 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     7921 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     7981 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     8041 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     8101 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     8161 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     8221 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     8281 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     8341 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgatggc cgcaataaaa
     8401 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gcgataagga
     8461 tccgcgtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     8521 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     8581 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     8641 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     8701 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     8761 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     8821 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     8881 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8941 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     9001 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     9061 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     9121 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     9181 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     9241 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     9301 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9361 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     9421 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     9481 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9541 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     9601 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9661 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     9721 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9781 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9841 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9901 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9961 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    10021 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    10081 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    10141 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    10201 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10261 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10321 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10381 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10441 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10501 tcctggcctt ttgctggcct tttgctcaca tggctcgaca gatct
//
