LOCUS       sequence-288819-       10533 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Insect Expression of HsTRC6C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10533)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 10533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-HsTNRC6C_G
FEATURES             Location/Qualifiers
     source          1..10533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4996..5013
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             7818..7859
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7869..7886
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7909..7926)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7959..8093
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7983..8002)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8037..8056
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8715..9303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8795..8814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9474..10265,10266..10334))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10097..10116
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10335..10439)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10507..10525
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgatgg ctacagggag tgcccagggc aacttcactg gacataccaa
     2761 gaagacaaat ggcaataatg gcaccaatgg cgcactcgtc caaagccctt ctaatcagag
     2821 tgcccttgga gcagggggag cgaacagtaa tggaagtgcg gccagagtgt ggggtgtagc
     2881 cacaggctcc agctctggcc tggctcactg ctctgtcagt ggtggggatg gaaaaatgga
     2941 cactatgatt ggagatggga gaagtcagaa ttgctggggt gcttccaact ccaatgctgg
     3001 cattaatctt aaccttaatc ctaatgccaa cccagctgcc tggcctgtac ttggacatga
     3061 aggaaccgtg gcgacaggca acccttccag tatttgcagt ccagtcagtg ccataggtca
     3121 aaatatgggc aaccagaacg ggaacccaac aggcacttta ggtgcttggg gaaacttgct
     3181 gccacaagag agcacagaac cacaaacgtc cacttctcag aatgtgtctt tcagcgcaca
     3241 acctcagaac cttaacactg atggaccaaa taacactaac cccatgaact cttcacccaa
     3301 ccctatcaat gcaatgcaga caaatggact gccaaactgg ggcatggctg ttggtatggg
     3361 ggccatcatc ccgccccacc tgcaaggcct tcctggtgct aatggatcat cagtttctca
     3421 agtcagtggg ggcagtgctg aaggaataag caattctgtg tggggactgt ccccaggtaa
     3481 ccctgccaca ggaaatagca attctgggtt cagtcagggg aatggagaca ctgtgaactc
     3541 agcattaagt gctaaacaaa atggatccag cagtgctgtg caaaaggaag gaagtggagg
     3601 aaatgcttgg gattcaggac ctcctgctgg tcctggaata ctcgcctggg gaaggggcag
     3661 tggcaacaat ggcgttggta atatccattc aggagcttgg ggccacccca gccgaagcac
     3721 ctctaacggt gtgaatgggg aatggggaaa gcccccaaac cagcattcca acagtgacat
     3781 caatgggaaa ggatcaacag ggtgggagag tcctagtgtc accagccaga accctaccgt
     3841 acagcctggt ggtgaacaca tgaactcctg ggccaaagcg gcatcttctg gaactacagc
     3901 aagtgaagga agtagtgatg gttctggcaa ccacaatgaa ggaagcactg ggagggaagg
     3961 aacgggagaa ggccgaaggc gagataaagg gattatagac caagggcaca tccagttgcc
     4021 aaggaatgat cttgacccaa gagttctgtc taatactggt tggggacaga ctcctgtaaa
     4081 gcaaaacact gcctgggaat ttgaagaatc ccctaggtct gaaaggaaaa atgacaatgg
     4141 gacagaggcc tggggttgtg cagctactca ggcttcaaac tcagggggga agaacgatgg
     4201 gtccatcatg aacagtacaa atacctcttc agtatctggg tgggtcaacg cgccacctgc
     4261 cgctgtgcca gcaaacacag gttggggaga cagcaacaac aaagcgccaa gtggcccggg
     4321 ggtttggggg gactcgataa gctctactgc tgttagtact gctgctgctg ccaagagtgg
     4381 ccatgcttgg agtggggccg caaatcagga ggacaagtca cccacctggg gtgagcctcc
     4441 aaagcccaaa tcccaacact ggggagatgg acaaagatca aatccagcct ggagtgcagg
     4501 agggggagat tgggcagatt catcgtctgt ccttggacac ttgggggatg ggaaaaaaaa
     4561 tggatctgga tgggatgctg acagtaatag gtcagggtct ggttggaatg acaccacgag
     4621 atctgggaac agtggctggg gcaacagcac aaatacaaag gccaatccag gtacaaactg
     4681 gggggagact ttaaaacctg gcccccaaca gaactgggct agcaaacccc aagacaacaa
     4741 tgtgagtaac tggggaggag ctgcttctgt gaaacagaca ggaacagggt ggatcggggg
     4801 gccggtaccg gtcaaacaga aggacagcag tgaagcaact ggctgggaag aaccctctcc
     4861 accgtccatt cgccgcaaaa tggaaattga tgatggtacc tcagcttggg gggacccaag
     4921 caactataac aataaaactg taaacatgtg ggatagaaac aacccggtca tccagagcag
     4981 taccacgacc aataccacca ccaccaccac cactaccacg agcaacacca cacacagggt
     5041 cgagacgccg cccccgcacc aggctggtac tcagctgaat cgatcaccgt tgcttggtcc
     5101 aggtaggaaa gtttcatcag gctggggaga aatgcctaat gttcactcaa agactgaaaa
     5161 ctcttgggga gaaccatcct ccccttctac cctggtggat aatggcacag cagcatgggg
     5221 gaagccaccc agcagtggca gcgggtgggg agatcaccct gccgagccgc cggtggcatt
     5281 tggaagagct ggcgcacctg ttgctgcctc agccctgtgc aaaccagctt caaaatctat
     5341 gcaagaaggc tggggcagtg gtggggatga aatgaacctc agtaccagcc agtgggagga
     5401 tgaagaaggg gacgtgtgga ataatgctgc ttcccaagaa agcacctcct cctgcagctc
     5461 ctgggggaac gcccccaaaa aaggacttca aaagggcatg aagacgtctg gcaagcagga
     5521 tgaggcctgg atcatgagcc ggctgatcaa acaactcaca gacatgggct tcccgagaga
     5581 gccagctgag gaggccttga agagtaacaa tatgaatctt gatcaggcca tgagcgctct
     5641 gctggaaaag aaggtggacg tggacaagcg tgggctggga gtgaccgacc ataatggaat
     5701 ggccgccaag cccctcggct gccgcccgcc aatctccaaa gagtcttccg tggaccgccc
     5761 cacctttctt gacaaggatg gcggcctcgt ggaagagccc acgccttcac cgttcttgcc
     5821 ttccccaagc ctgaagctcc ccctttcaca cagtgcactc cccagtcagg ccctgggtgg
     5881 gattgcctcc gggctgggca tgcaaaactt gaattcttct agacagatac cgagtggcaa
     5941 tctgggtatg tttggcaata gtggagcagc acaagccagg accatgcagc agccgccaca
     6001 gccaccagtg cagcctctta actcttccca gcccagtctc cgtgctcaag tgcctcagtt
     6061 tctatcccct caggttcaag cacagctttt gcagtttgca gcaaaaaaca ttggtctcaa
     6121 ccctgcacta ttaacctcgc caattaatcc tcaacatatg acgatgttga accagctcta
     6181 tcagctgcag ctggcatacc aacgtttaca aatccagcag cagatgttac aggcccagcg
     6241 taatgtgtcc ggatccatga gacaacagga gcagcaagtt gcgcgcacaa tcactaatct
     6301 gcagcagcag atccagcagc accagcgcca gctggcccag gccctgctcg tgaagcagcc
     6361 accaccgcca ccgcccccgc cgcacctgtc tctgcacccc tctgcaggca aatcggccat
     6421 ggacagcttc ccctcgcacc cacagactcc cggcctacct gacctgcaga ccaaagagca
     6481 gcagtcttca cccaacacct ttgctcctta ccctctcgct ggactgaacc caaacatgaa
     6541 tgtcaacagc atggacatga ccggtggctt gtcggtgaag gacccatccc agtcccagtc
     6601 acgcctcccc cagtggacgc accccaactc catggataac ttgcccagtg ccgcttcccc
     6661 cctggagcag aaccctagca agcatggtgc tatccctgga ggtctaagca ttgggcctcc
     6721 aggtaagtcc tccattgatg actcctatgg ccggtacgat ttaatccaga acagtgagtc
     6781 accagccagt cctcccgtag ctgttcccca tagctggtca cgtgccaaat ctgacagtga
     6841 taaaatctca aatggctcta gcatcaactg gcccccagaa ttccatccgg gagttccatg
     6901 gaaaggactg cagaatattg accctgagaa tgaccctgac gtcactcctg gcagtgtccc
     6961 cactgggcct accatcaaca ccaccatcca ggatgtcaac cgctacctcc tcaagagtgg
     7021 aggtaaactg tcagacatca aatcgacgtg gtcctctggc cctacctccc acacgcaagc
     7081 ctctctgtct catgaactat ggaaggtgcc cagaaacagt actgcaccca cgaggccacc
     7141 tccagggtta accaatccca agccctcctc cacctggggt gccagccccc tcggctggac
     7201 cagctcctac tcctcgggtt ctgcctggag caccgacacc tcaggaagaa ccagcagctg
     7261 gctcgttctt cgaaacctca ctccccagat tgatggttct acactgcgga cattgtgttt
     7321 gcaacatggg cctcttatca cattccacct gaatctgact caaggcaatg ctgtggtccg
     7381 gtacagctcc aaggaggagg ctgccaaggc ccagaagtct ctgcacatgt gcgtcctggg
     7441 aaacactacc atcctggccg agttcgctgg tgaagaagaa gtgaatcgct tcttagccca
     7501 aggccaggcg ctgccaccca cttccagctg gcagtccagc agcgcgtcca gccagccgcg
     7561 gctcagcgca gcgggcagct cccatggcct ggtacgcagc gacgctggcc actggaacgc
     7621 cccgtgcctg ggtggcaagg ggagcagtga gctgctgtgg ggcggggtgc cccagtactc
     7681 cagcagcctg tggggcccgc ccagcgccga cgacagcagg gtgataggca gccccacgcc
     7741 gctaaccacc ctgctgcctg gggacctgct cagcggggag tccctgtagc tcgagtctag
     7801 agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
     7861 gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc
     7921 cttctaaggc ctgagctcgc tgatcagcct cgatcgagga tccagacatg ataagataca
     7981 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     8041 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     8101 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     8161 agtaaaacct ctacaaatgt ggtatggctg attatgatca gtcgaccgat gcccttgaga
     8221 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     8281 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     8341 ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     8401 ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     8461 gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     8521 ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     8581 gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     8641 cttcaaggat cgctcgcggc tcttaccagc cagcaaaagg ccaggaaccg taaaaaggcc
     8701 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8761 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8821 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8881 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     8941 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9001 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9061 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9121 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     9181 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9241 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9301 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9361 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9421 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     9481 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9541 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9601 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9661 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9721 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9781 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9841 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9901 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9961 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10021 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10081 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10141 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10201 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10261 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10321 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    10381 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    10441 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
    10501 tataaaaata ggcgtatcac gaggcccttt cgt
//
