LOCUS       sequence-288884-        9278 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Insect Expression of DmGW182-delM2-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9278)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-delM2-dsRNAres_H
FEATURES             Location/Qualifiers
     source          1..9278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2589..2654
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2682..2708
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6563..6604
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6614..6631
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6654..6671)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6704..6838
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6728..6747)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6782..6801
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7460..8048)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7540..7559)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8219..9010,9011..9079))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8842..8861
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9080..9184)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9252..9270
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacccaacat ggacgcacaa acacgacgac gtgagcgtcg cgctgagaaa caagctcaat
     2641 ggaaagctgc aaacggtgga tctcctccaa gcttgatggc ttacccatac gatgttccag
     2701 attacgctat gcgtgaagcc cttttttccc aagatggctg gggctgtcag catgttaacc
     2761 aggatactaa ttgggaagtt cccagttcgc cagaaccagc caataaggat gcacccggtc
     2821 caccaatgtg gaagccaagc attaacaatg gtactgatct ttgggagtcc aatttgagaa
     2881 acggaggtca gccggccgca cagcaagttc caaagccgtc gtggggtcat acaccatcct
     2941 ctaacttagg tggaacatgg ggtgaggacg acgatggcgc cgatagtagt agtgtgtgga
     3001 ctggaggagc tgttagcaac gcgggatccg gagctgcagt gggagtaaac caagccggag
     3061 ttaatgtcgg tccaggcggt gttgtttcgt ctggcggacc tcagtgggga caaggtgtcg
     3121 ttggcgtcgg acttggatca actggcggaa atggttccag taacattaca ggctcttcgg
     3181 gcgttgccac gggaagcagt ggcaattcaa gtaatgccgg aaatggatgg ggcgatccac
     3241 gcgagattcg tccactgggc gtcggaggat cgatggacat ccgtaacgtc gagcaccgtg
     3301 gaggaaatgg atcaggcgcc acatccagtg acccgcgtga tatccgtatg attgacccac
     3361 gcgatccaat ccggggcgac ccacgcggca tttcaggacg cctcaacggt acgtcagaga
     3421 tgtggggaca ccacccgcag atgtcgcaca atcaactgca gggaattaat aagatggtcg
     3481 gacagagcgt tgcgacagct agtacgagcg ttggcacgtc aggatccgga attggaccag
     3541 gcggaccggg accaagcacg gtttcgggaa acattccgac gcaatggggt ccagcccagc
     3601 ccgttagtgt cggagttagc ggaccgaagg atatgtcgaa gcaaattagc ggctgggaag
     3661 agccgtcgcc tcccccacaa cgccgtagca tcccaaatta tgacgacgga acgtccctct
     3721 ggggacaaca gacacgcgtc ccggccgcta gtggacattg gaaggatatg acagactcca
     3781 ttggacgcag cagccacctg atgcgcggac agagtcagac cggcggaatt ggcattgctg
     3841 gagttggaaa tagcaatgtt ccagtgggag ccaatccaag taatcctata agcagtgtag
     3901 ttggacctca agcccggatt ccatctgtgg gcggcgtaca acacaaacca gacggcggcg
     3961 ctatgtgggt gcattccggc aatgtaggtg gcagaaataa tgttgctgct gttactactt
     4021 ggggagatga cactcatagc gttaatgtcg gcgctcccag cagtggcagt gtatccagca
     4081 acaattgggt tgatgacaag tccaactcaa ccttggcaca aaactcttgg agcgacccgg
     4141 cccctgttgg agttagttgg ggcaataagc aaagcaaacc gccaagcaat agtgcttcat
     4201 caggttggag cactgctgcg ggcgtggtgg atggggttga tctaggatct gagtggaaca
     4261 cgcacggggg gattattgga aaatctcagc aacaacaaaa actagcggga cttaacgtgg
     4321 gaatggtgaa cgtaattaac gcggagatca ttaagcaaag caagcaatac aggatccttg
     4381 tcgagaacgg ctttaaaaag gaagatgtag agcgggcatt agtgattgct aatatgaaca
     4441 tcgaagaggc agccgatatg ctccgtgcca actcatccct atcaatggat ggttggcgtc
     4501 gacatgatga gtcccttgga tcttatgccg accacaatag ttcaacaagc agcggtggat
     4561 ttgctggtcg ttacccggtc aacagtggac aaccttcaat gtcctttcct cataataacc
     4621 ttatgaataa catgggaggt accgctgtta ctggaggtaa caacaataca aacatgacag
     4681 ctttacaggt gcaaaagtat ttaaatcaag ggcaacatgg tgtcgctgtt ggaccgcaag
     4741 ccgttggtaa ttcttcagca gtatctgtcg gatttggtca gaacacgtct aacgcagcag
     4801 tggcaggagc agcctctgca aatatagcag caaatacaaa caaccaaccg tctggtcagc
     4861 aaattcgcat gctaggccag caaattcagt tggccattca tagtggtttc atatctagtc
     4921 agatattgac tcaaccgcta actcaaacaa cccttaacct tttaaaccaa cttcttagca
     4981 atattaagca tctccaggct gcacagcaat cccttacccg cgggggaaat gtcaatccaa
     5041 tggcagtgaa tgtggctata tctaaataca agcagcaaat ccagaattta cagaaccaga
     5101 taagtgcaca acaggctgtg tatgtaaaac agcaaaatat gcaaccaact tcacaacaac
     5161 aacagcccca acaacagcaa cttccttctg ttcatctaag taactcaggc aacgactatt
     5221 taagaggtca cgatgcaata aataatttgc aaagcaactt ttctgagctc aatattaata
     5281 agccaagtgg atatcaagga gcgtccaatc aacaatcccg attaaatcag tggaagcttc
     5341 cagtattaga taaggagatc aactctgaca gtacggaatt ttctcgtgcc ccaggtgcaa
     5401 cgaaacaaaa tttgacggcc aacacaagca acataaactc tttgggtctt caaaacgata
     5461 gtacatggtc aactggacgc agtattggtg acggttggcc tgatccctca tctgataacg
     5521 agaataaaga ctggtctgtt gctcagccaa cttcagcagc aactgcttac actgatctgg
     5581 tccaagagtt tgagccaggc aagccatgga agggttcaca gatcaaaagc atagaagatg
     5641 atcccagcat tacaccagga agcgttgcta gatctccatt gtctattaat tcgacgccaa
     5701 aagatgctga catattttcc agttggttgc ttttgaaaaa tctaacagca cagattgatg
     5761 gtcctacttt gcgtacactg tgtatgcagc atgggcccct tgtcagcttt cacccgtatt
     5821 tgaaccaagg aattgcctta tgtaaatata ctactcgtga ggaggcgaac aaggcgcaaa
     5881 tggcgttaaa caactgtgtc ctcgccaaca ccacaatatt tgctgaatct cccagcgaga
     5941 acgaggtgca aagcattatg cagcacttac cacaaactcc ttcctctaca agctctagtg
     6001 gaactagtgg tggcaacgtc ggaggcgtcg gcacttcagc caataatgca aacagtggtt
     6061 ctgcagcttg tctgtccgga aacaatagcg gcaacggaaa cggcagcgcg agcggcgccg
     6121 gcagcggcaa caatggcaac agtagctgca acaacagtgc cgccgggggg ggcagcagca
     6181 gcaacaacac gattaccact gtagcaaatt cgaatcttgt tggttctagt ggctctgtct
     6241 caaattcctc tggcgttact gctaactcta gtactgtttc tgtagttagt tgtacagcga
     6301 gtgggaattc cataaatggg gcaggtactg caaacagttc tggttcaaag agtagtgcaa
     6361 acaatttagc tagcggccag tctagcgctt ctaacttaac taatagcacc aattcaacat
     6421 ggcgacaaac tagccaaaac caagctcttc aaagtcaaag caggccatca ggcagagaag
     6481 ctgactttga ttatatatct ctcgtttatt ccattgttga tgattaagcg gccgctcgag
     6541 tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc
     6601 tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac
     6661 tgtgccttct aaggcctgag ctcgctgatc agcctcgatc gaggatccag acatgataag
     6721 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     6781 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     6841 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     6901 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagtcga ccgatgccct
     6961 tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
     7021 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg
     7081 tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg
     7141 tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt
     7201 tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc
     7261 tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg
     7321 gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg
     7381 gacagcttca aggatcgctc gcggctctta ccagccagca aaaggccagg aaccgtaaaa
     7441 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7501 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7561 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7621 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     7681 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7741 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7801 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7861 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     7921 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7981 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8041 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8101 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8161 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8221 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8281 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8341 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8401 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8461 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8521 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8581 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8641 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8701 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8761 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8821 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8881 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8941 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9001 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9061 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9121 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9181 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     9241 taacctataa aaataggcgt atcacgaggc cctttcgt
//
