LOCUS       sequence-288941-        6633 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Insect Expression of DmGW182-M2-RBD-Cterm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6633)
  AUTHORS   Zekri L, Huntzinger E, Heimstadt S, Izaurralde E
  TITLE     The silencing domain of GW182 interacts with PABPC1 to promote 
            translational repression and degradation of miRNA targets and is 
            required for target release.
  JOURNAL   Mol Cell Biol. 2009 Sep 21. ():.
  PUBMED    19797087
REFERENCE   2  (bases 1 to 6633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2009 Sep 21. ():."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-M2-RBD-Cterm_H
FEATURES             Location/Qualifiers
     source          1..6633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     161..180
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(399..503)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     571..589
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        653..3165
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             3185..3250
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             3275..3301
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4515..4556
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4566..4583
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4606..4623)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4656..4790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4680..4699)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4734..4753
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5412..6000)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5492..5511)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6171..6633,1..329,330..398))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
       61 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
      121 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
      181 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
      241 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
      301 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
      361 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
      421 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
      481 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
      541 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
      601 caaggaatta attctatatt ctaaaaacac aaatgatact tctaaaaaaa atcatgaatg
      661 gcatcaactc tgaatcaaat ctttgcagat gcacctactt ctcatttcca ctgtcacatc
      721 atttttccag atctcgctgc ctgttatgtg gcccacaaac caagacacgt tttatggcca
      781 ttaaagctgg ctgatcgtcg ccaaacacca aatacatatc aatatgtaca ttcgagaaag
      841 aagcgatcaa agaagcgtct tcgggcgagt aggagaatgc ggaggagaag gagaacgagc
      901 tgatctagta tctctccaca atccaatgcc aactgaccaa ctggccatat tcggagcaat
      961 ttgaagccaa tttccatcgc ctggcgatcg ctccattctt ggctatatgt ttttcaccgt
     1021 tcccggggcc attttcaaag actcgtcggt aagataagat tgtgtcactc gctgtctctc
     1081 ttcatttgtc gaagaatgct gaggaatttc gcgatgacgt cggcgagtat tttgaagaat
     1141 gagaataatt tgtatttata cgaaaatcag ttagtggaat tttctacaaa aacatgttat
     1201 ctatagataa ttttgttgca aaatatgttg actatgacaa agattgtatg tatatacctt
     1261 taatgtattc tcattttctt atgtatttat aatggcaatg atgatactga tgatatttta
     1321 agatgatgcc agaccacagg ctgatttctg cgtcttttgc cgaacgcagt gcatgtgcgg
     1381 ttgttgtttt ttggaatagt ttcaattttc ggactgtccg ctttgatttc agtttcttgg
     1441 cttattcaaa aagcaaagta aagccaaaaa agcgagatgg caataccaaa tgcggcaaaa
     1501 cggtagtgga aggaaagggg tgcggggcag cggaaggaag ggtggggcgg ggcgtggcgg
     1561 ggtctgtggc tgggcgcgac gtcaccgacg ttggagccac tcctttgacc atgtgtgcgt
     1621 gtgtgtatta ttcgtgtctc gccactcgcc ggttgttttt ttctttttat ctcgctctct
     1681 ctagcgccat ctcgtacgca tgctcaacgc accgcatgtt gccgtgtcct ttatgcgtca
     1741 ttttggctcg aaataggcaa ttatttaaac aaagattagt caacgaaaac gctaaaataa
     1801 ataagtctac aatatggtta cttattgcca tgtgtgtgca gccaacgata gcaacaaaag
     1861 caacaacaca gtggctttcc ctctttcact ttttgtttgc aagcgcgtgc gagcaagacg
     1921 gcacgaccgg caaacgcaat tacgctgaca aagagcagac gaagttttgg ccgaaaaaca
     1981 tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc gatatttaat attgtttatg
     2041 aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa caaattattt gaaagagaat
     2101 taggaatcgg acagcttatc gttacgggct aacagcacac cgagacgaaa tagcttacct
     2161 gacgtcacag cctctggaag aactgccgcc aagcagagag agagagaaaa agagggagag
     2221 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctcc tgtttgcgca
     2281 aacgcataga ctgcactgag aaaatcgatt acctattttt tatgaatgaa tatttgcact
     2341 attactattc aaaactatta agatagcaat cacattcaat agccaaatac tataccacct
     2401 gagcgatgca acgaaatgat caatttgagc aaaaatgctg catatttagg acggcatcat
     2461 tatagaaatg cttcttgctg tgtacttttc tctcgtctgg cagctgtttc gccgttattg
     2521 ttaaaaccgg cttaagttag gtgtgttttc tacgactagt gatgccccta ctagaagatg
     2581 tgtgttgcac aaatgtccct gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2641 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2701 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2761 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2821 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2881 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2941 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     3001 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     3061 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     3121 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     3181 caacatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
     3241 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
     3301 tcctccaagc ttgaattctg cagatatcca gcacagtggc ggccgcgcca ataccggtaa
     3361 aaattcaccg actgatttac cgccactaag tttatcgtcg tctacatgga gttttaatcc
     3421 aaaccaaaat tatccgagtc acagttggtc tgacaatagt caacaatgta ccgccacttc
     3481 ggagctttgg acaagcccgc taaataaatc atcgtctcga ggtcccccgc caggattgac
     3541 tgccaattca aataaatctg caaatagtaa tgcgtcaacg ccaacaacta ttaccggagg
     3601 tgcgaatgga tggttacagc ctcgaagtgg cggtgttcaa accacaaaca ctaattggac
     3661 aggtggtaac accacttggg gctccagttg gttgcttttg aaaaatctaa cagcacagat
     3721 tgatggtcct actttgcgta cactgtgtat gcagcatggg ccccttgtca gctttcaccc
     3781 gtatttgaac caaggaattg ccttatgtaa atatactact cgtgaggagg cgaacaaggc
     3841 gcaaatggcg ttaaacaact gtgtcctcgc caacaccaca atatttgctg aatctcccag
     3901 cgagaacgag gtgcaaagca ttatgcagca cttaccacaa actccttcct ctacaagctc
     3961 tagtggaact agtggtggca acgtcggagg cgtcggcact tcagccaata atgcaaacag
     4021 tggttctgca gcttgtctgt ccggaaacaa tagcggcaac ggaaacggca gcgcgagcgg
     4081 cgccggcagc ggcaacaatg gcaacagtag ctgcaacaac agtgccgccg gggggggcag
     4141 cagcagcaac aacacgatta ccactgtagc aaattcgaat cttgttggtt ctagtggctc
     4201 tgtctcaaat tcctctggcg ttactgctaa ctctagtact gtttctgtag ttagttgtac
     4261 agcgagtggg aattccataa atggggcagg tactgcaaac agttctggtt caaagagtag
     4321 tgcaaacaat ttagctagcg gccagtctag cgcttctaac ttaactaata gcaccaattc
     4381 aacatggcga caaactagcc aaaaccaagc tcttcaaagt caaagcaggc catcaggcag
     4441 agaagctgac tttgattata tatctctcgt ttattccatt gttgatgatt aatctagagg
     4501 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
     4561 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     4621 ctaaggcctg agctcgctga tcagcctcga tcgaggatcc agacatgata agatacattg
     4681 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     4741 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     4801 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     4861 aaaacctcta caaatgtggt atggctgatt atgatcagtc gaccgatgcc cttgagagcc
     4921 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     4981 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc
     5041 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga
     5101 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag
     5161 aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc
     5221 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
     5281 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt
     5341 caaggatcgc tcgcggctct taccagccag caaaaggcca ggaaccgtaa aaaggccgcg
     5401 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5461 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5521 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5581 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     5641 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5701 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5761 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5821 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     5881 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5941 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6001 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6061 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6481 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6601 ttcggtcctc cgatcgttgt cagaagtaag ttg
//
