LOCUS       sequence-289047-       10483 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian Expression of HsTNRC6C-delRRM-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10483)
  AUTHORS   Eulalio A, Helms S, Fritzsch C, Fauser M, Izaurralde E
  TITLE     A C-terminal silencing domain in GW182 is essential for miRNA 
            function.
  JOURNAL   RNA. 2009 Apr 21. ():.
  PUBMED    19383769
REFERENCE   2  (bases 1 to 10483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Apr 21. ():."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6C-delRRM-siRNAres_I
FEATURES             Location/Qualifiers
     source          1..10483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1197..1223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3530..3547
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6187..6308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6198..6217)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6252..6271
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6494..6949
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6581..6600)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6791..6812
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7066..7423
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7066..7257
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7089..7109)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7274..7409
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7474..8268
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7528..7547)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8138..8157
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8332..8380
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8421..8440)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8540..8562
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8600..8618)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8686..8790
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8791..8859,8860..9651)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9009..9028)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9822..10410
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10311..10330
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg aggctgccca agcttacacc atggcctacc
     1201 catatgatgt tccagattac gcttcactcg acgcggatcc cgtcgaattc atggctacag
     1261 ggagtgccca gggcaacttc actggacata ccaagaagac aaatggcaat aatggcacca
     1321 atggcgcact cgtccaaagc ccttctaatc agagtgccct tggagcaggg ggagcgaaca
     1381 gtaatggaag tgcggccaga gtgtggggtg tagccacagg ctccagctct ggcctggctc
     1441 actgctctgt cagtggtggg gatggaaaaa tggacactat gattggagat gggagaagtc
     1501 agaattgctg gggtgcttcc aactccaatg ctggcattaa tcttaacctt aatcctaatg
     1561 ccaacccagc tgcctggcct gtacttggac atgaaggaac cgtggcgaca ggcaaccctt
     1621 ccagtatttg cagtccagtc agtgccatag gtcaaaatat gggcaaccag aacgggaacc
     1681 caacaggcac tttaggtgct tggggaaact tgctgccaca agagagcaca gaaccacaaa
     1741 cgtccacttc tcagaatgtg tctttcagcg cacaacctca gaaccttaac actgatggac
     1801 caaataacac taaccccatg aactcttcac ccaaccctat caatgcaatg cagacaaatg
     1861 gactgccaaa ctggggcatg gctgttggta tgggggccat catcccgccc cacctgcaag
     1921 gccttcctgg tgctaatgga tcatcagttt ctcaagtcag tgggggcagt gctgaaggaa
     1981 taagcaattc tgtgtgggga ctgtccccag gtaaccctgc cacaggaaat agcaattctg
     2041 ggttcagtca ggggaatgga gacactgtga actcagcttt gagcgccaag cagaatggat
     2101 ccagcagtgc tgtgcaaaag gaaggaagtg gaggaaatgc ttgggattca ggacctcctg
     2161 ctggtcctgg aatactcgcc tggggaaggg gcagtggcaa caatggcgtt ggtaatatcc
     2221 attcaggagc ttggggccac cccagccgaa gcacctctaa cggtgtgaat ggggaatggg
     2281 gaaagccccc aaaccagcat tccaacagtg acatcaatgg gaaaggatca acagggtggg
     2341 agagtcctag tgtcaccagc cagaacccta ccgtacagcc tggtggtgaa cacatgaact
     2401 cctgggccaa agcggcatct tctggaacta cagcaagtga aggaagtagt gatggttctg
     2461 gcaaccacaa tgaaggaagc actgggaggg aaggaacggg agaaggccga aggcgagata
     2521 aagggattat agaccaaggg cacatccagt tgccaaggaa tgatcttgac cctagggtcc
     2581 tctccaacac tggttgggga cagactcctg taaagcaaaa cactgcctgg gaatttgaag
     2641 aatcccctag gtctgaaagg aaaaatgaca atgggacaga ggcctggggt tgtgcagcta
     2701 ctcaggcttc aaactcaggg gggaagaacg atgggtccat catgaacagt acaaatacct
     2761 cttcagtatc tgggtgggtc aacgcgccac ctgccgctgt gccagcaaac acaggttggg
     2821 gagacagcaa caacaaagcg ccaagtggcc cgggggtttg gggggactcg ataagctcta
     2881 ctgctgttag tactgctgct gctgccaaga gtggccatgc ttggagtggg gccgcaaatc
     2941 aggaggacaa gtcacccacc tggggtgagc ctccaaagcc caaatcccaa cactggggag
     3001 atggacaaag atcaaatcca gcctggagtg caggaggggg agattgggca gattcatcgt
     3061 ctgtccttgg acacttgggg gatgggaaaa aaaatggatc tggatgggat gctgacagta
     3121 ataggtcagg gtctggttgg aatgacacca cgagatctgg gaacagtggc tggggcaaca
     3181 gcacaaatac aaaggccaat ccaggtacaa actgggggga gactttaaaa cctggccccc
     3241 aacagaactg ggctagcaaa ccccaagaca acaatgtgag taactgggga ggagctgctt
     3301 ctgtgaaaca gacaggaaca gggtggatcg gggggccggt accggtcaaa cagaaggaca
     3361 gcagtgaagc aactggctgg gaagaaccct ctccaccgtc cattcgccgc aaaatggaaa
     3421 ttgatgatgg tacctcagct tggggggacc caagcaacta taacaataaa actgtaaaca
     3481 tgtgggatag aaacaacccg gtcatccaga gcagtaccac gaccaatacc accaccacca
     3541 ccaccactac cacgagcaac accacacaca gggtcgagac gccgcccccg caccaggctg
     3601 gtactcagct gaatcgatca ccgttgcttg gtccaggtag gaaagtttca tcaggctggg
     3661 gagaaatgcc taatgttcac tcaaagactg aaaactcttg gggagaacca tcctcccctt
     3721 ctaccctggt ggataatggc acagcagcat gggggaagcc acccagcagt ggcagcgggt
     3781 ggggagatca ccctgccgag ccgccggtgg catttggaag agctggcgca cctgttgctg
     3841 cctcagccct gtgcaaacca gcttcaaaat ctatgcaaga aggctggggc agtggtgggg
     3901 atgaaatgaa cctcagtacc agccagtggg aggatgaaga aggggacgtg tggaataatg
     3961 ctgcttccca agaaagcacc tcctcctgca gctcctgggg gaacgccccc aaaaaaggac
     4021 ttcaaaaggg catgaagacg tctggcaagc aggatgaggc ctggatcatg agccggctga
     4081 tcaaacaact cacagacatg ggcttcccga gagagccagc tgaggaggcc ttgaagagta
     4141 acaatatgaa tcttgatcag gccatgagcg ctctgctgga aaagaaggtg gacgtggaca
     4201 agcgtgggct gggagtgacc gaccataatg gaatggccgc caagcccctc ggctgccgcc
     4261 cgccaatctc caaagagtct tccgtggacc gccccacctt tcttgacaag gatggcggcc
     4321 tcgtggaaga gcccacgcct tcaccgttct tgccttcccc aagcctgaag ctcccccttt
     4381 cacacagtgc actccccagt caggccctgg gtgggattgc ctccgggctg ggcatgcaaa
     4441 acttgaattc ttctagacag ataccgagtg gcaatctggg tatgtttggc aatagtggag
     4501 cagcacaagc caggaccatg cagcagccgc cacagccacc agtgcagcct cttaactctt
     4561 cccagcccag tctccgtgct caagtgcctc agtttctatc ccctcaggtt caagcacagc
     4621 ttttgcagtt tgcagcaaaa aacattggtc tcaaccctgc actattaacc tcgccaatta
     4681 atcctcaaca tatgacgatg ttgaaccagc tctatcagct gcagctggca taccaacgtt
     4741 tacaaatcca gcagcagatg ttacaggccc agcgtaatgt gtccggatcc atgagacaac
     4801 aggagcagca agttgcgcgc acaatcacta atctgcagca gcagatccag cagcaccagc
     4861 gccagctggc ccaggccctg ctcgtgaagc agccaccacc gccaccgccc ccgccgcacc
     4921 tgtctctgca cccctctgca ggcaaatcgg ccatggacag cttcccctcg cacccacaga
     4981 ctcccggcct acctgacctg cagaccaaag agcagcagtc ttcacccaac acctttgctc
     5041 cttaccctct cgctggactg aacccaaaca tgaatgtcaa cagcatggac atgaccggtg
     5101 gcttgtcggt gaaggaccca tcccagtccc agtcacgcct cccccagtgg acgcacccca
     5161 actccatgga taacttgccc agtgccgctt cccccctgga gcagaaccct agcaagcatg
     5221 gtgctatccc tggaggtcta agcattgggc ctccaggtaa gtcctccatt gatgactcct
     5281 atggccggta cgatttaatc cagaacagtg agtcaccagc cagtcctccc gtagctgttc
     5341 cccatagctg gtcacgtgcc aaatctgaca gtgataaaat ctcaaatggc tctagcatca
     5401 actggccccc agaattccat ccgggagttc catggaaagg actgcagaat attgaccctg
     5461 agaatgaccc tgacgtcact cctggcagtg tccccactgg gcctaccatc aacaccacca
     5521 tccaggatgt caaccgctac ctcctcaaga gtggaggtaa actgtcagac atcaaatcga
     5581 cgtggtcctc tggccctacc tcccacacgc aagcctctct gtctcatgaa ctatggaagg
     5641 tgcccagaaa cagtactgca cccacgaggc cacctccagg gttaaccaat cccaagccct
     5701 cctccacctg gggtgccagc cccctcggct ggaccagctc ctactcctcg ggttctgcct
     5761 ggagcaccga cacctcagga cccacttcca gctggcagtc cagcagcgcg tccagccagc
     5821 cgcggctcag cgcagcgggc agctcccatg gcctggtacg cagcgacgct ggccactgga
     5881 acgccccgtg cctgggtggc aaggggagca gtgagctgct gtggggcggg gtgccccagt
     5941 actccagcag cctgtggggc ccgcccagcg ccgacgacag cagggtgata ggcagcccca
     6001 cgccgctaac caccctgctg cctggggacc tgctcagcgg ggagtccctg taggcgctgc
     6061 catcatcagc accaggagag ccgacccctc ccgggacccc tcccggctgg gcggccccac
     6121 agacccgctg gaacccagca gcggccgctt ccctttagtg agggttaatg cttcgagcag
     6181 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     6241 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     6301 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     6361 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatccgataa ggatcgatcc
     6421 gggctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     6481 gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6541 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6601 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6661 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6721 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6781 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6841 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6901 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt
     6961 attttctcct tacgcatctg tgcggtattt cacaccgcat acgcggatct gcgcagcacc
     7021 atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa
     7081 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     7141 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     7201 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     7261 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7321 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7381 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg attcttctga
     7441 cacaacagtc tcgaacttaa ggctagagcc accatgattg aacaagatgg attgcacgca
     7501 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     7561 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     7621 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     7681 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     7741 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     7801 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     7861 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     7921 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     7981 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     8041 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     8101 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     8161 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     8221 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     8281 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgatggccg caataaaata
     8341 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagc gataaggatc
     8401 cgcgtatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     8461 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     8521 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     8581 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     8641 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8701 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8761 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8821 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8881 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     8941 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     9001 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     9061 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     9121 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     9181 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     9241 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     9301 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     9361 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     9421 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     9481 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     9541 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     9601 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     9661 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9721 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9781 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9841 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9901 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9961 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    10021 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    10081 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    10141 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    10201 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    10261 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    10321 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    10381 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    10441 ctggcctttt gctggccttt tgctcacatg gctcgacaga tct
//
