LOCUS       sequence-289439-        9017 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Insect Expression of DmUPF1-S1121A-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9017)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmUPF1-S1121A-siRNAres-V5Hi...
FEATURES             Location/Qualifiers
     source          1..9017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      4210..4219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6302..6343
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6353..6370
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6393..6410)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6443..6577
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6467..6486)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6521..6540
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7199..7787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7279..7298)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7958..8749,8750..8818))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8581..8600
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8819..8923)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8991..9009
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catgagcgtg gacacgtacg cgcccagctc
     2761 ggcgctctcg ttcctggaca tggacgacaa cgagctgctt ccgggagcgg atactcaacc
     2821 cacgcagtac gattaccgcg actttaccat gccctccacc tcgcagagcc agacccagaa
     2881 cgatcagctg gagattgccc aacgctgctc tgccggagac tcgcatccac gactggccag
     2941 catcaccaac gatctggccg atctgcagtt cgaagaggag gacgacgagc ctggcagctc
     3001 gtatgtgaag gagctgccgc cgcatgcgtg caagtattgc ggcatccatg atccagccac
     3061 ggtggtcatg tgcaacaact gccgcaaatg gttttgcaac ggtcgtggaa gcacttccgg
     3121 ttcgcacatc atcaaccatc tggtgagggc caagcatcgc gaggtgacgc tccacgggga
     3181 aggtcccctg ggcgagacaa tcctggagtg ctactcctgt ggtgtgcgca acgtctttgt
     3241 gctgggcttc attccggcca aggccgattc tgtggtcgtg ctgctctgcc gtcagccgtg
     3301 tgccgcccaa aactccctta aagacatgaa ctgggaccag gaacagtgga agcctctaat
     3361 tgcagaccgc tgctttttgg cctggctggt aaagcaaccc agcgaacagg gacagctgcg
     3421 agctcgccaa atctcagccg ctcagatcaa caagctggag gagctatgga aggagaatat
     3481 tgaggccacg tttcaggatc tggagaagcc aggcattgac tcggagccag cacatgtgct
     3541 actccgctac gaggatggct atcagtacga gaagaccttt gggccgctgg tccgccttga
     3601 ggccgaatac gaccaaaaac tgaaggagtc tgccacgcag gagaacatcg aagtacgctg
     3661 ggacgtcggc ctcaacaaaa agaccattgc ctactttacg ctggcgaaga ccgattcgga
     3721 catgaagctc atgcatggcg acgagctgcg cctgcattat gtgggcgagc tgtacaatcc
     3781 gtggagcgag atcggccacg ttatcaaggt gccggacaat ttcggcgatg acgtcggcct
     3841 ggagctgaaa tcctcaacga atgccccggt taagtgcacc agtaacttta cggtggactt
     3901 catctggaag tgcacgtcat ttgatcgcat gacacgtgct ctgtgcaaat tcgccatcga
     3961 tcgcaattca gtgtcgaact tcatctactc gcgcctgttg ggccacggtc gtgcggattc
     4021 caacgacgag gtgctgttcc gcggcccaca acccaagctc ttcagtgccc cgcatctgcc
     4081 ggatttgaat cgcagccagg tgtatgccgt gaaacacgcg cttcagcgtc cgctctcgct
     4141 aatccaaggg ccgcctggca cgggcaaaac cgtgacctcg gcgaccatcg tttaccagct
     4201 ggtcaagctc catggtggca cagtgctggt gtgcgctccc agcaacacgg ccgtggatca
     4261 gctaactgag aagatccacc gaacaaacct taaagtggtg cgtgtttgcg ccaagagccg
     4321 tgaggccatc gatagcccgg taagcttcct ggcgctgcac aaccaaatcc gtaacatgga
     4381 gaccaactcg gagctaaaga agctgcagca gctgaaagac gagaccggcg agctgagctc
     4441 agcagacgaa aagcgatacc gcaacctgaa acgtgccgcc gagaaccaac tgctggaggc
     4501 tgccgacgtt atctgctgca catgcgtagg cgccggcgat ggtcgtctat cgcgagtcaa
     4561 gttcacctcg atcctgatcg atgagtctat gcagtcgacg gagccggagt gcatggtgcc
     4621 agtggtgctg ggcgctaagc agctgatcct cgtgggcgat cactgccagc tgggaccggt
     4681 tgttatgtgc aagaaagcag ctcgtgccgg cctctcgcaa agtttgttcg agcgcctggt
     4741 ggttctgggc atccgtccgt tccggctgga ggtgcaatat cgcatgcacc ccgagctgtc
     4801 ccagttcccg tccaacttct tctacgaggg atcgctgcaa aacggcgtct gcgcggagga
     4861 tcgtcgcctt aagcttgatt tcccctggcc acagccggag agaccgatgt tctttctggt
     4921 aacacaggga caggaggaga ttgccggctc cggcacctct ttcctcaacc gcacagaggc
     4981 ggccaacgtg gaaaagatta cgacgcgatt ccttaaggca ggcatcaagc cggaacaaat
     5041 tggaatcatc acgccttacg aaggtcagcg cgcttacctg gtgcagtaca tgcaatacca
     5101 gggcagcctg cactctcgtc tataccagga gatcgagatc gccagtgtgg acgcgttcca
     5161 gggacgtgag aaggacatca ttatcatgtc ttgcgtgcga tctaacgaac gtcaaggcat
     5221 cggctttttg aacgacccac gtcgccttaa tgttgccctt acacgggcca agttcgggat
     5281 catcattgtg ggcaatccca aagtgctcgc caagcagcag ctgtggaacc atctgctcaa
     5341 cttctacaag gaccgtaagg tgctcgtcga gggatctctg aataacctta aggagtcgct
     5401 aatccacttc cagaagccca aaaagcttgt caacagcatg aacattgggg cacactttat
     5461 gtccaccatt attgccgatg ccaaggaagt gatggtgcca ggctccattt acgaccgcag
     5521 tggcggttac ggccaaggtc gccaaatggt gggacagtca atgaatggcg gacagtacgg
     5581 tggcagtgga ggtggtccct acggaaactc acccctcggc tacggtactc ccagctccaa
     5641 ttccatggtg ggctttggcc tgggcaacgg aggcaatggc gcggccggtg gcaacaacaa
     5701 cttcggaggg gctgaaccca gttgggcggc tgcccacctc caccacgact ccattggcta
     5761 tatatccaac gagcatggag cagcagcact gggcaacatg ccagttccgg ttggcatgtt
     5821 catgaacatg agcaatattc cgccgcgttt ctacaaccag caccagcagg cgatcatggc
     5881 ggtcaagcag aatcgcgcca ttcaacaaca gacgggtaat ttctctcccg gtaactcggg
     5941 tcctggagtc actggagtcg gagtcggacg aagcgccacc ccaggcggca ataagaagac
     6001 caacaagctg ggaaaatcgc gcgtaacggg cggtggaact ggcggggcac cgctaacaca
     6061 aggaagctcg gtatgcaatg ctgctccata cgctcagcac ccgatgcctt tgtcgctgca
     6121 gatgacccag cccagcggat ttgctctgtc ccagcagccg gaactttcac aggactttgg
     6181 gcaaatatcg cagatggacg gtttgctatc ccaggatgtt gcctttaacg cgtcgggcga
     6241 gcggagcttg aatcagttct cacagcctta tcgctcgagt ctagagggcc cgcggttcga
     6301 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca
     6361 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta aggcctgagc
     6421 tcgctgatca gcctcgatcg aggatccaga catgataaga tacattgatg agtttggaca
     6481 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     6541 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     6601 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     6661 atgtggtatg gctgattatg atcagtcgac cgatgccctt gagagccttc aacccagtca
     6721 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     6781 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     6841 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     6901 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
     6961 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
     7021 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
     7081 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
     7141 cggctcttac cagccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7201 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7261 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7321 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7381 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7441 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7501 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7561 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7621 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7681 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7741 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7801 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7861 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7921 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7981 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8041 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8101 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8161 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8221 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     8281 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8341 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8401 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8461 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8521 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     8581 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8641 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     8701 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8761 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     8821 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8881 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8941 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     9001 tcacgaggcc ctttcgt
//
