LOCUS       sequence-290668-        8178 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Insect Expression of DmRRP6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8178)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmRRP6_AA
FEATURES             Location/Qualifiers
     source          1..8178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5463..5504
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5514..5531
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5554..5571)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5604..5738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5628..5647)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5682..5701
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6360..6948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6440..6459)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7119..7910,7911..7979))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7742..7761
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7980..8084)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8152..8170
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catgccgcga accccgaaaa gagttcacca
     2761 ggaggccaaa gaggaatcgg cccaagcaga ccagccgcca aagaaatccg cttcagaaga
     2821 tgttgaggca ttcacaaaca aagggttcaa gaatgcaatc gccgccacaa aagccgctaa
     2881 tgctttccca caaggaacag cccgcgccct ttacctaagc tatccaggct acgcacgtgt
     2941 tatggaagac ctgacccaac gggtggtggc gctcatcggg aacgtgctgc actccaagga
     3001 catcaagggc gacatcaaaa aacgccaacc ggaagagcag tttgagatgg tgcaggagtg
     3061 caacgacgta ctgttcgagc gcattacgac taatctagac attaaaggtg gtctgcgaag
     3121 aaatacacaa caggtcgtag aggcccaggt ggacgtgatg agcagctcca catccataga
     3181 gcccgcagta gcttccccac aaacccaagg gactccaaag gcaggcagct ggaacagaac
     3241 gacaggaaca ccccaacgca gcatggtttc ggctcgtctc ttcaccgcca agaacatcgt
     3301 tcggccgcag acgcaattta gggagccggt ggataacagt gcccagaatc cgtttgttcc
     3361 aagactcaag gagaaaccta attctcttaa accgctggcc ctgctgcccg aatacgatga
     3421 tgccggcaat gtccaatcct atttgcatcc ctacgagttt gagctgctta agtttcagcc
     3481 tccggaggag cagttccaaa aacaaaagcc agtgcttcca gctctgatgg cagaaaccga
     3541 actaatggtg gttgacacgg tggaaaaact gaagcaggca ttggaggaac tgagacaagc
     3601 gccccagatc gccatagatg tggagcacca ctcgtatcgc accttcatgg gcatcacttg
     3661 cctggtccag atgtctactc gcagcaaaga ctatattttt gataccctga tcctacgcga
     3721 tgatatgcat atactaaatt tggtactcac cgatcccaag aagctgaaga tactgcacgg
     3781 cgccgatttg gatattgagt ggcttcagcg ggacttatcg ttgtatatcg tcaacatgtt
     3841 cgacacgcat cgggctgcca aagccttgaa tatggcaagg ttatcgttgg catatttgct
     3901 taagcactat ctggacttgg atgtggacaa gagcctgcaa ctggccgatt ggcggatgag
     3961 accgcttccc cagcagctgg tggactacgc tcgtcaggat acccactttt tgatatacgt
     4021 ttacggacgg atgacaaacg atctgctgca gcagcatgcc gaaccaggtc tgctgggcag
     4081 tgtctatcaa cagagcacgg atgtctgcaa gaagcgttat aataagcctc acatcggacc
     4141 agaatcccat ttggatttag taagaaaaac gaagcgctct ttcgacaacc ggcaactgta
     4201 cgcacttcgc ggaatcttcg agtggcgcga tgcgacggcg cggtcagagg acgagagtta
     4261 cggctatgtg ctgcccaatc acatgatgct gcaaatcgct gagagtcttc caagggagat
     4321 gcagggaatc ctcgcctgct gcaatcccat tccgccgctg gtgcgccaac agctgcacac
     4381 gcttcaccag attgtcctca aggcgcgcga tcagccgctg gtcaaaccga tcctggaagc
     4441 ccacagtagt acacaggcag cgttgcctcc gtcgaccaaa gactttagca gcaagctcta
     4501 ctgtccgcac gatttttcgc aactggagga gattcgcgac gacctgccaa cgctgttgaa
     4561 acgtaattca actacaggaa aactggaggt gcccaataag gaggaggttg ccaaagtaga
     4621 tccaacctta gcggcaccag ccatggctct gttcgaaaag cagcccaagc cgacacaaga
     4681 agaggaacag cgctgggccc atcttcgcaa ggagagccag acaatgcgta tgccctacaa
     4741 acgctatctc gccatcctac ctctgatggt gcagctgaag gccgatcagt tggccaggga
     4801 gcgaagtgag ttgcaaaaga ggcaattgtg tccagcagcc ccaactgtgg agcaaaacat
     4861 taagctggag gcacacgcca tcggaaagga ggatgatgac atgtactccg tcccgctgaa
     4921 ggaacaactg aagcgcaagc acccacaagc gaacgtgaaa acagatccgg aacagcagcc
     4981 gactgttagc aagcgacctc gcaaggacga gaattcacaa accaaaccgc cggtaaagat
     5041 cgaaccagtc gaaaaagttc agcaggcttt aggcgaaagc gatgatgagg tcgttgaggt
     5101 tcctatcgaa aggcaagcca cagagccacc gaaaccatca ccggcacaga acaaccgcaa
     5161 gcaaaagaaa aatcagttcc agcgcggatt caaggccaag aatcgaggaa accacccgca
     5221 gagctccagt cagcaggttc cgcagcacaa gggtacaggc aactttgact acaagaacgt
     5281 ggacttccgg caattcaagg gaggagcgca aagagcccgg ggcacagaaa tcaagcagca
     5341 aattcgcggc aagaaccgtc ccaacaatcg gaacaacaag cagttcaaca agctttttac
     5401 atttagtaat gtaagaaaag agggcaaaaa atgactcgag tctagagggc ccgcggttcg
     5461 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc
     5521 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct aaggcctgag
     5581 ctcgctgatc agcctcgatc gaggatccag acatgataag atacattgat gagtttggac
     5641 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     5701 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     5761 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     5821 aatgtggtat ggctgattat gatcagtcga ccgatgccct tgagagcctt caacccagtc
     5881 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     5941 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
     6001 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
     6061 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
     6121 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
     6181 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
     6241 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
     6301 gcggctctta ccagccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6541 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6781 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6901 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6961 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7021 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7081 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7141 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7201 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7261 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7321 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7381 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
     7441 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7501 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7561 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7621 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7681 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
     7741 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7801 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7861 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7921 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7981 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8041 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8101 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     8161 atcacgaggc cctttcgt
//
