LOCUS       sequence-291317-        7210 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian Expression of HsZNF598-C29A-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7210)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-MS2-HA-C1-HsZNF598-C29A-siRNAres_AH
FEATURES             Location/Qualifiers
     source          1..7210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1097..1123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3998..4119
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4035..4054)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4089..4108
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4126..4581)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4263..4284)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4475..4494
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4608..4712
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4714..5071
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4714..4905
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4737..4757)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4922..5057
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5106..5900
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5106..5900
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5160..5179)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5770..5789
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6088..6107)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6132..6179
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6508..7096
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6997..7016
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggct tctaacttta ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt
      721 gactgtcgcc ccaagcaact tcgctaacgg ggtcgctgaa tggatcagct ctaactcgcg
      781 atcacaggct tacaaagtaa cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata
      841 caccatcaaa gtcgaggtgc ctaaagtggc aacccagact gttggtggtg aagagcttcc
      901 tgtagccgga tggagatctt acttaaatat ggaactaacc attccaattt tcgccacgaa
      961 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat
     1021 tccctcggcc atcgcggcca actccggcat ctacggaggt ggaggtggat ctggtggatc
     1081 tcctccacat atggcttacc catacgatgt tccagattac gctcctccat ctcgagctat
     1141 ggcggcggcg gggggcgccg aggggcggcg cgcggccctg gaggcggcgg cggcggcagc
     1201 tcctgagcgg ggaggcggga gcgccgtgct gtgctgcgga gacctggagg ccacggcgct
     1261 gggccgctgc gaccacccgg tgtgctaccg ctgctctacc aagatgcggg tgctctgcga
     1321 gcagcgctac tgcgccgtgt gccgcgagga gctgcgccag gtggtctttg ggaagaagct
     1381 tcctgccttt gccaccatcc ccatccacca gctgcagcat gagaagaaat atgatatcta
     1441 ctttgcagat ggaaaggtgt acgcattgta caggcagctg ctgcagcacg agtgcccgcg
     1501 gtgccccgag ctgccacctt tcagcctctt cggggacctg gagcagcaca tgcggaggca
     1561 gcatgagctc ttctgctgcc gtctgtgcct ccagcacctc cagatcttca catatgagcg
     1621 caagtggtac tcgcgcaagg acctggcccg gcatcgcatg cagggtgacc ccgatgacac
     1681 gtcgcaccgt gggcacccgc tctgcaagtt ctgtgacgag cgctacctgg acaatgatga
     1741 gctgcttaag cacctgcgcc gcgaccacta cttctgccac ttctgcgact cggacggggc
     1801 ccaggactac tacagcgact atgcctacct gcgtgagcac ttccgggaga agcactttct
     1861 gtgtgaggaa ggccgctgca gcacggagca gttcacccac gccttccgca ccgagatcga
     1921 cctcaaggcc cacaggacgg cctgccacag tcgcagccgc gccgaggcac gccagaaccg
     1981 ccacatcgac ctgcagttca gctacgcgcc acggcactcg cgccggaacg agggggtcgt
     2041 tggtggcgaa gactacgagg aggtggacag gtacagccgc cagggccgag tggcccgggc
     2101 tggcactcgc ggagcccagc agagccgccg aggaagctgg aggtacaaaa gggaagaaga
     2161 ggaccgagaa gtagcagctg ctgtccgggc ctccgtggcc gcacagcagc aggaggaggc
     2221 ccgaagaagc gaagaccagg aggaaggcgg taggccaaaa aaggaagaag cggcggcgcg
     2281 gggacctgag gatccccgtg gcccccggcg ctcaccccgg actcagggcg aaggcccagg
     2341 ccccaaggaa acctcgacaa atggtcctgt aagccaagaa gccttctcgg tgacaggccc
     2401 agccgcccca gggtgtgtgg gggtgccagg cgccctccca ccacccagcc cgaagctcaa
     2461 ggacgaagac ttccccagcc tctctgcctc cacttcctcc tcctgctcca ctgcagcaac
     2521 cccgggccct gtggggttgg cgctgccgta cgccatccct gccagaggca ggagtgcctt
     2581 ccaggaggag gacttccccg ccctggtgtc ctcggtgccc aagcctggca ccgcccccac
     2641 cagccttgtc tctgcctgga acagcagcag tagcagcaag aaggtagcac agcccccact
     2701 ctcggcgcag gctaccggca gcggccagcc caccaggaag gctgggaagg ggagcagggg
     2761 cggcaggaag ggcggcccgc ccttcacaca ggaggaggag gaggacggcg gcccggccct
     2821 gcaggagctt ctgagcacac gccccacggg ctccgtctcc tccacactgg ggctggcctc
     2881 catccaaccc tctaaagttg ggaagaagaa gaaagtgggc tcggagaagc caggcaccac
     2941 attgccacag cccccgcccg ctacctgtcc cccaggggct ttgcaggccc cggaagctcc
     3001 tgccagcaga gccgaggggc cagttgccgt cgtcgttaat ggacacacgg agggcccggc
     3061 ccctgctcgg agtgccccca aggaaccccc tgggctccca aggcccctgg ggtccttccc
     3121 ctgccccacg ccacaggagg acttcccagc gctcggcggc ccctgcccac cccggatgcc
     3181 gccgccccca ggcttcagcg ctgtggtgct cctgaagggc acgcctcccc cacccccgcc
     3241 gggcctggtg cccccaatca gcaagccgcc ccctggcttc tctggccttc tgcctagccc
     3301 ccacccggcc tgtgtcccca gccccgccac caccaccacc acaaaagcac ccaggctgct
     3361 gcctgcccca cgggcgtacc tagtccccga gaacttccgg gagaggaacc ttcagctcat
     3421 ccagtccatc agggacttcc tgcagagcga cgaggcccgc ttcagcgagt tcaagagcca
     3481 ctcaggggag ttcagacagg gcctgatctc cgcagcccag tattacaaga gttgccggga
     3541 cctgctgggg gagaatttcc agaaggtctt taatgagctg ctggtcctgc tgcccgacac
     3601 ggccaagcag caggagctcc tgtctgcaca cacggacttc tgcaaccgcg agaagcctct
     3661 gagcaccaag tccaagaaga acaagaagag cgcgtggcag gccaccaccc agcaggcggg
     3721 cctggactgc cgtgtgtgcc ccacctgcca gcaggtgctc gcgcatggcg atgccagcag
     3781 ccaccaggcg ctgcatgctg cccgggacga cgacttcccc tccctgcaag ccatcgccag
     3841 gatcatcacg tagggtaccg cgggcccggg atccaccgga tctagataac tgatcataat
     3901 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     3961 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     4021 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4081 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta
     4141 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     4201 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     4261 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     4321 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     4381 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     4441 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     4501 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     4561 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4621 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4681 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     4741 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4801 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4861 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4921 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4981 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5041 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     5101 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     5161 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     5221 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     5281 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     5341 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     5401 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     5461 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     5521 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5581 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     5641 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5701 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5761 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5821 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5881 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     5941 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     6001 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     6061 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     6121 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     6181 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     6241 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     6301 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     6361 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6421 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6481 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6541 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6601 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     6661 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6721 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6781 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6841 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6901 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6961 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7021 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7081 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7141 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7201 cgccatgcat
//
