LOCUS       sequence-291319-        6448 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian Expression of HsZNF598-del611-864-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6448)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-MS2-HA-C1-HsZNF598-del611-864-siRNAres_AH
FEATURES             Location/Qualifiers
     source          1..6448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1097..1123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3236..3357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3273..3292)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3327..3346
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3364..3819)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3501..3522)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3713..3732
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3846..3950
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3952..4309
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3952..4143
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3975..3995)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4160..4295
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4344..5138
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4344..5138
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4398..4417)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5008..5027
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5326..5345)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5370..5417
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5746..6334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6235..6254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggct tctaacttta ctcagttcgt tctcgtcgac aatggcggaa ctggcgacgt
      721 gactgtcgcc ccaagcaact tcgctaacgg ggtcgctgaa tggatcagct ctaactcgcg
      781 atcacaggct tacaaagtaa cctgtagcgt tcgtcagagc tctgcgcaga atcgcaaata
      841 caccatcaaa gtcgaggtgc ctaaagtggc aacccagact gttggtggtg aagagcttcc
      901 tgtagccgga tggagatctt acttaaatat ggaactaacc attccaattt tcgccacgaa
      961 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat
     1021 tccctcggcc atcgcggcca actccggcat ctacggaggt ggaggtggat ctggtggatc
     1081 tcctccacat atggcttacc catacgatgt tccagattac gctcctccat ctcgagctat
     1141 ggcggcggcg gggggcgccg aggggcggcg cgcggccctg gaggcggcgg cggcggcagc
     1201 tcctgagcgg ggaggcggga gctgcgtgct gtgctgcgga gacctggagg ccacggcgct
     1261 gggccgctgc gaccacccgg tgtgctaccg ctgctctacc aagatgcggg tgctctgcga
     1321 gcagcgctac tgcgccgtgt gccgcgagga gctgcgccag gtggtctttg ggaagaagct
     1381 tcctgccttt gccaccatcc ccatccacca gctgcagcat gagaagaaat atgatatcta
     1441 ctttgcagat ggaaaggtgt acgcattgta caggcagctg ctgcagcacg agtgcccgcg
     1501 gtgccccgag ctgccacctt tcagcctctt cggggacctg gagcagcaca tgcggaggca
     1561 gcatgagctc ttctgctgcc gtctgtgcct ccagcacctc cagatcttca catatgagcg
     1621 caagtggtac tcgcgcaagg acctggcccg gcatcgcatg cagggtgacc ccgatgacac
     1681 gtcgcaccgt gggcacccgc tctgcaagtt ctgtgacgag cgctacctgg acaatgatga
     1741 gctgcttaag cacctgcgcc gcgaccacta cttctgccac ttctgcgact cggacggggc
     1801 ccaggactac tacagcgact atgcctacct gcgtgagcac ttccgggaga agcactttct
     1861 gtgtgaggaa ggccgctgca gcacggagca gttcacccac gccttccgca ccgagatcga
     1921 cctcaaggcc cacaggacgg cctgccacag tcgcagccgc gccgaggcac gccagaaccg
     1981 ccacatcgac ctgcagttca gctacgcgcc acggcactcg cgccggaacg agggggtcgt
     2041 tggtggcgaa gactacgagg aggtggacag gtacagccgc cagggccgag tggcccgggc
     2101 tggcactcgc ggagcccagc agagccgccg aggaagctgg aggtacaaaa gggaagaaga
     2161 ggaccgagaa gtagcagctg ctgtccgggc ctccgtggcc gcacagcagc aggaggaggc
     2221 ccgaagaagc gaagaccagg aggaaggcgg taggccaaaa aaggaagaag cggcggcgcg
     2281 gggacctgag gatccccgtg gcccccggcg ctcaccccgg actcagggcg aaggcccagg
     2341 ccccaaggaa acctcgacaa atggtcctgt aagccaagaa gccttctcgg tgacaggccc
     2401 agccgcccca gggtgtgtgg gggtgccagg cgccctccca ccacccagcc cgaagctcaa
     2461 ggacgaagac ttccccagcc tctctgcctc cacttcctcc tcctgctcca ctgcagcaac
     2521 cccgggccct gtggggttgg cgctgccgta cgccatccct gccagaggca ggagtgcctt
     2581 ccaggaggag gacttccccg ccctggtgtc ctcggtgccc aagcctggca ccgcccccac
     2641 cagccttgtc tctgcctgga acagcagcag tagcagcaag aaggtagcac agcccccact
     2701 ctcggcgcag gctaccggca gcggccagcc caccaggaag gctgggaagg ggagcagggg
     2761 cggcaggaag ggcggcccgc ccttcacaca ggaggaggag gaggacggcg gcccggccct
     2821 gcaggagctt ctgagcacac gccccacggg ctccgtctcc tccacactgg ggctggcctc
     2881 catccaaccc tctaaagttg ggaagaagaa gaaagtgggc tcggagaagc caggcaccac
     2941 attgccacag cccccgcccg ctacctgtcg tgtgtgcccc acctgccagc aggtgctcgc
     3001 gcatggcgat gccagcagcc accaggcgct gcatgctgcc cgggacgacg acttcccctc
     3061 cctgcaagcc atcgccagga tcatcacgta gggtaccgcg ggcccgggat ccaccggatc
     3121 tagataactg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     3181 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     3241 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3301 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg
     3361 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     3421 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     3481 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     3541 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     3601 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     3661 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     3721 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     3781 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     3841 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3901 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     3961 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4021 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4081 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4141 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4201 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4261 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     4321 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     4381 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4441 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4501 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     4561 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     4621 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     4681 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     4741 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     4801 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     4861 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     4921 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     4981 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5041 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5101 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     5161 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     5221 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     5281 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     5341 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     5401 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     5461 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     5521 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     5581 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5641 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5701 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5761 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5821 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5881 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5941 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6001 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6061 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6121 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6181 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6241 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6301 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6361 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6421 tgtggataac cgtattaccg ccatgcat
//
