LOCUS       sequence-291837-       10702 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses Flag-tagged Chibby1 in multiciliated cells under FoxJ1 
            promoter. It can be used as a cloning vector for gene of interest at
            SfiI sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10702)
  TITLE     Expresses Flag-hChibby1 in multiciliated cells under FoxJ1 promoter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-FoxJ1 promoter-IRES-EGFP-Flag-hChibby1
FEATURES             Location/Qualifiers
     source          1..10702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3007..3016
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3016..3039
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(3461..4035,4036..4038,4039..4047)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3628..3645)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3855..3874
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4048..4050,4051..4053,4054..4767)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4048..4050,4051..4053,4054..4764)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4093..4114)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4354..4373)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4701..4722
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4777..5365
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4830..4850)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5248..5259)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5889..6069
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6095..6112)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6101..6325
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6371..6799
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6458..6477)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6668..6689
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6808..6828)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6813..7142
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6993..7128
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7055..7074
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7190..7237
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7256..7630
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7760..7881
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7797..7816)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7851..7870
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7930..7946)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7930..7946)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7943..7965)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7954..7970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7978..7984,7985..8002,8003..8008))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8023..8044
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8161..8178)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8332..8920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8412..8431)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9091..9882,9883..9951))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9714..9733
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9952..10056)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10131..10150)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10322..10701
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taatgagccg agccgggact tagcggcggg tagggggttg ggtggcgcgg gagagggcgg
     2041 cagaaggagg gagttcgaag agagttgccg ccagggagag tggcaagtgc ccgcggtctc
     2101 cttggagacg tgctggccaa tggcagaggc ggaagctgtt tatgggggcg gagcgtcgcc
     2161 atggcagcag gagaagcctt tggagcggct acttaatgcc ggtcccgggg ccgcggtagc
     2221 tcagagaagc gccgtcgggc gggggggcgg caagaggcgc tggcagggcc cccgagctgg
     2281 gggcagagag tcgcaggggg tgggagcaac ttcttccaga accttcctct ggcccgggct
     2341 gcgctctgag ccaggtaagg gatggggagg gctgcaggcc agggggaagg gtgggcaacg
     2401 gtaccaaccc gctctctcat tcgtccttaa cggaagtggt gggcccaggg actgacatcg
     2461 gagaagtctg ggggaaactc gcctcctgcc ccccgttact gttccgtccc cttccttccc
     2521 aatgatcccc tatcataata ttccttttca aggatcagag gacctccacc gcccccgccc
     2581 cgtcgtaccg ccctcccaaa cttctcgccc cacttccatt tgttgcgagg aggaaggggg
     2641 tcctgatttc ctgctcttcg ggagaaaagg atagcctcgg atgcccctac cctcttcatt
     2701 tggctaataa aacctcgaag aggaaaggaa gagggaggca gatctgatgg gggtgggacc
     2761 ccagggcctt gcttctgttt cccatacctc ggcactaaaa gatcctcccc agctgcgggg
     2821 atgatgttag agagggcgtc cccgtacaca cactgtcccc tcgggggtgg ggtagtgctc
     2881 agctcccacc gtggtcctcg caggcaccac atacttattc ggaggaggcg cccccggggt
     2941 cgcgagatgg aagcgcgcgt ccgggaggcc gtgtaacggg agctgcggga gaattcggcc
     3001 attacggcca ccatggatta caaggatgac gacgataaga tctcgaattc gatgcctttc
     3061 tttgggaata cgttcagtcc gaagaagaca cctcctcgga agtcggcatc tctctccaac
     3121 ctgcattctt tggatcgatc aacccgggag gtggagctgg gcttggaata cggatccccg
     3181 actatgaacc tggcagggca aagcctgaag tttgaaaatg gccagtggat agcagagaca
     3241 ggggttagtg gcggtgtgga ccggagggag gttcagcgcc ttcgcaggcg gaaccagcag
     3301 ttggaggaag agaacaatct cttgcggctg aaagtggaca tcttattaga catgctttca
     3361 gagtccactg ccgaatccca cttaatggag aaggaactgg atgaactgag gatcagccgg
     3421 aagagaaaat gaggccgcct cggccatcga taaggatccg cccctctccc tccccccccc
     3481 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     3541 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     3601 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     3661 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     3721 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     3781 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     3841 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     3901 aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt
     3961 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     4021 aaacacgatg ataatatggc cacaaccatg gtgagcaagg gcgaggagct gttcaccggg
     4081 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     4141 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     4201 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     4261 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     4321 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     4381 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     4441 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     4501 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     4561 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     4621 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     4681 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     4741 ctcggcatgg acgagctgta caagtaaagc ggccgcaatc aacctctgga ttacaaaatt
     4801 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     4861 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     4921 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     4981 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5041 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5101 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     5161 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     5221 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5281 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     5341 atctcccttt gggccgcctc cccgcctgcc tgcaggtttg tcgagaccta gaaaaacatg
     5401 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     5461 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
     5521 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     5581 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     5641 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
     5701 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
     5761 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
     5821 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
     5881 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5941 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     6001 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     6061 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     6121 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6181 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6241 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     6301 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     6361 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6421 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6481 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6541 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6601 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6661 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6721 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6781 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     6841 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     6901 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6961 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     7021 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7081 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     7141 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     7201 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     7261 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     7321 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     7381 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     7441 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     7501 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     7561 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     7621 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     7681 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     7741 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     7801 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7861 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     7921 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     7981 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8041 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8101 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     8161 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     8221 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     8281 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8341 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8401 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8461 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8521 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8581 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8641 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8701 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8761 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8821 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8881 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8941 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     9001 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     9061 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     9121 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9181 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     9241 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9301 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9361 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9421 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9481 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9541 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9601 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9661 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9721 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9781 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9841 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9901 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9961 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    10021 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    10081 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
    10141 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
    10201 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
    10261 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
    10321 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
    10381 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
    10441 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
    10501 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
    10561 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
    10621 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
    10681 ttagtcatcg ctattaccat gg
//
