LOCUS       sequence-292602-        4416 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Module plasmid, individual component of Fungal Bioluminescent 
            Pathway, Luz.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: P212
FEATURES             Location/Qualifiers
     source          1..4416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      171..759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     660..679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     913..930
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1465..1810
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1753..1773
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      2632..3339
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        3415..3519
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgtgcgggc ttttttttgg tggtggttac cagtgtcgct tagctaccgt aggtcctctt
       61 aggcggtccg attagcgaca tagtcatcaa tgccttgacg gcaaaatccc ttaacgtgag
      121 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      181 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      241 tgtttgccgg atcaagagct actaactctt tttccgaagg taactggctt cagcagagcg
      301 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      361 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      421 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      481 tcgggctgaa cggggggttc gtgctcacag cccagcttgg agcgaacgac ctacaccgaa
      541 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      601 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      661 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      721 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      781 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      841 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
      901 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccacc tgcaacgtcc
      961 cggcgcgccc tatggacaga tttgcctttt caatttcaga aagaatgcta acccacagat
     1021 ggttagagag gcttacgcag caggtatcat caagacgatc tacccgagca ataatctcca
     1081 ggaaatcaaa taccttccca agaaggttaa agatgcagtc aaaagattca ggactaactg
     1141 catcaagaac acagagaaag atatatttct caagatcaga agtactattc cagtatggac
     1201 gattcaaggc ttgcttcaca aaccaaggca agtaatagag attggagtct ctaaaaaggt
     1261 agttcccact gaatcaaagg ccatggagtc aaagattcaa atagaggacc taacagaact
     1321 cgccgtaaag actggcgaac agttcataca gagtctctta cgactcaatg acaagaagaa
     1381 aatcttcgtc aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
     1441 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
     1501 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
     1561 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
     1621 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
     1681 tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga
     1741 cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt
     1801 ggagagaaca cgggggacta atgcgtataa atatctctct gtctagctta ttcgagagat
     1861 taagcaaact cagctctcgt agcatagcta ttacatgtgg tgtcgtcctc gcctctgcta
     1921 ttgcttttcc catcatacga cgagattatc agacgttctt agaggttggt cctagctatg
     1981 cccctcaaaa ttttcgaggc tacattatcg tttgcgtact cagcttgttc cgacaagagc
     2041 aaaaagggtt ggcaatctac gacagactcc cagagaagag aagatggctg gccgatctcc
     2101 catttcgtga aggtactaga cctagtataa catcacacat aatacagaga cagcgaactc
     2161 aacttgtaga ccaagagttt gccacacgag aactgataga caaagttatt cccagggtgc
     2221 aagcacgaca tacagataaa acatttttga gcacgagcaa gtttgaattt catgctaaag
     2281 ccattttcct gttgccaagt attcctataa acgacccctt aaacatacct agtcatgata
     2341 cggttcgtag aacgaaacga gagattgcac acatgcatga ctaccacgac tgtactcttc
     2401 acttagctct tgcagctcaa gatggaaaag aagtcctgaa aaaaggctgg ggccaacgtc
     2461 atccgcttgc cgggccgggt gtacctgggc cgccgacaga atggactttc ttatacgcac
     2521 cccgtaatga ggaagaagct agggtagtag aaatgattgt cgaggcaagt atcggataca
     2581 tgactaatga tccggcaggt aaaatcgtcg agaatgccaa atgagcttgt cctgctttaa
     2641 tgagatatgc gagaagccta tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt
     2701 gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt cattctaatg
     2761 aatatatcac ccgttactat cgtattttta tgaataatat tctccgttca atttactgat
     2821 tgtaccctac tacttatatg tacaatatta aaatgaaaac aatatattgt gctgaatagg
     2881 tttatagcga catctatgat agagcgccac aataacaaac aattgcgttt tattattaca
     2941 aatccaattt taaaaaaagc ggcagaaccg gtcaaaccta aaagactgat tacataaatc
     3001 ttattcaaat ttcaaaagtg ccccaggggc tagtatctac gacacaccga gcggcgaact
     3061 aataacgctc actgaaggga actccggttc cccgccggcg cgcatgggtg agattccttg
     3121 aagttgagta ttggccgtcc gctctaccga aagttacggg caccattcaa cccggtccag
     3181 cacggcggcc gggtaaccga cttgctgccc cgagaattat gcagcatttt tttggtgtat
     3241 gtgggcccca aatgaagtgc aggtcaaacc ttgacagtga cgacaaatcg ttgggcgggt
     3301 ccagggcgaa ttttgcgaca acatgtcgag gctcagcagg accgctgtta gagctcagtg
     3361 actagcaggt gatgaaaagc cttagggtca ggtggcactt ttcggggaaa tgtgcgatga
     3421 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctaat gagacaataa
     3481 ccctgataaa tgcttcaatt atattgaaaa aggaagagta tgagtatcca acatttccgt
     3541 gtcgccctta ttcccttttt tgcggccttc tgccttcctg tttttgctca cccagaaacg
     3601 ctggtgaaag taaaagatgc tgaagatcag ttgggtgccc gagtgggtta cattgaactg
     3661 gatctcaaca gcggcaaaat cttagagagt tttcgccccg aagaacgctt cccaatgatg
     3721 agcactttca aagttctgct atgtggcgcg gtgttatccc gtattgatgc tgggcaagag
     3781 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtattc accagtcaca
     3841 gaaaagcatc ttacggatgg catgacagta agagaattat gtagtgctgc cataaccatg
     3901 agtgataaca ctgcggccaa cttacttctg acaacgattg gaggaccgaa ggagctaacc
     3961 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4021 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaact
     4081 ctccgcaaac tattaactgg cgaactactt actcttgctt cacgccagca actcattgac
     4141 tggatggagg cggacaaagt tgcaggacca cttctgcgct cggcacttcc ggctggctgg
     4201 tttattgctg ataaatctgg agccggtgag cgtggctctc gcggtatcat tgcagcactg
     4261 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4321 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4381 ctgtcagacg aagggaaata aatagtagcc cgcctg
//
