LOCUS       sequence-296015-       10557 bp    DNA     circular SYN 14-APR-2025
DEFINITION  a targeting plasmid for APEX-PML.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10557)
  AUTHORS   Kurihara M, Kato K, Sanbo C, Shigenobu S, Ohkawa Y, Fuchigami T, 
            Miyanari Y
  TITLE     Genomic Profiling by ALaP-Seq Reveals Transcriptional Regulation by 
            PML Bodies through DNMT3A Exclusion.
  JOURNAL   Mol Cell. 2020 May 7;78(3):493-505.e8. doi: 
            10.1016/j.molcel.2020.04.004. Epub 2020 Apr 29.
  PUBMED    32353257
REFERENCE   2  (bases 1 to 10557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.04.004"; journalName: "Mol Cell"; date: 
            "2020-05-7- 7"; volume: "78"; issue: "3"; pages: "493-505.e8"
COMMENT     Sequence Label: pBlue-loxPuro-APEX-PML_LASA
FEATURES             Location/Qualifiers
     source          1..10557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3540..3573)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3580..4176
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3580..3599)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4076..4096
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4195..4251
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(4252..4285)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5050..5076
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(7691..7707)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7691..7707)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7744..7764)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7744..7762)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7783..7799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7783..7799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7796..7818)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7807..7823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7831..7837,7838..7855,7856..7861))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7876..7897
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8014..8031)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8185..8773)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8265..8284)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8944..9735,9736..9804))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9567..9586
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9805..9909)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9935..10390)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10072..10093)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10284..10303
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10517..10539
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10531..10548
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10532..10548
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggt accgggcccc ccccccactg cttccggctt
       61 tactccgtca ctaacaacag tggctgagta ggatcaggaa tcaggccggg tccaccatgc
      121 ctgccgctcc acagcacccc ctgcgctggg aggaccgaac ggcgggagca cagtttgggg
      181 atgtggcctt ggttctaagg ttgaggcttc ctgaccacct cctacctggc cttaggaaag
      241 gaggcaagga agtttggctt taaaggagca ttgcaggagc gttttctcca gaagcagaga
      301 aatgtttttg gcctcaaacc attcctccaa actcaacaca cttatgagtt atgggtgtgc
      361 ttatccagat gtaggatttc ttctggtgtg gtcagcaaac tggtattttt tgtttgtttg
      421 tttccccaca gccaagaaaa cagagcctgc catacagagt cctgttctta tttgcttaat
      481 gatggcagat ccaagtcact gtggacaagt tgaagtcaga aggccccaac tttggtataa
      541 aagaaaaatt tcacatgcag acaccccagg aatcctggca gtggatggtg gtggtggtgg
      601 ctgcagcagc agcagcaaag cattacatga gactccttga tccaggcact tgaggtaact
      661 ttgtctttcc gtggcttcag aaagcccaaa tgaaggtaga aaatataccc cagtaggatg
      721 ctgggaggct ggcaggaggc cattttacaa agcaagaaaa gacgaatctt tcccagtctt
      781 gcctccctgg atccttgcca tagctcattt ctaagttccc ctgcctatga ctgctagcct
      841 ggctaatttt atgtcaactt gacacaagct agaataatct gaaaagaggg atctccaatt
      901 gagaaaatgg ttgtgctgta ggcaagcctg aagagcattt tcctaattta tgattgacgt
      961 tgcagggccc attccattgt gggtggggcc aaccctgggc tgctggtcct gggttcatta
     1021 agaaagcagg ctgaacaagc cagtaagcag catcctccat ggcctttgca gcagctcctg
     1081 catccaggtt cctgccctat ttgagtcctg gcttccctca ttgatggacc accatatgga
     1141 aatgtaagca aaataaagcc tttcctaccc aacttgctct ttactcacgg tgttttgtca
     1201 cagcaataaa aaccctaaga tgagttggta ccgggagtga ggttttaatt aatttcaaat
     1261 gtagtcaagt tgacaaccca gaattgccat acaagtccac cccttgccaa cttgacacac
     1321 aatcatatct tcttatcaat ttccaagtga aaacattaag caaatcataa taatacctaa
     1381 acatgatata actatctgac atacaattac aaacacatta tatttaacca tgttataatt
     1441 acacctaacc agataaaact atccctcata caactgcaaa tgcattgcaa atttagagta
     1501 gatgccaggc gtgacggtgc acgcctttaa tcccagcact cggaggcaga ggcaggcaga
     1561 tttctgagtt taagaccagg ctggtctaca aagtgagttc caggacagcc agggctgtac
     1621 agggaaaccc tgtcttgaaa aaccaaaaaa aaaacaaaaa acaaaaaaca aacaaaccaa
     1681 accaaacaaa caaacaaaaa caaacgaaca aacaaaaaat ttagagtaga tgacaatgtc
     1741 ccttgaggga cattcttttt tttttttttt tgaattaatt aatttatttc atgtatgtga
     1801 gtacactgtc gatgtcttca gacacaccag aagagggcat cagatcccat tacagatggt
     1861 tgtagccacc atgtggttgc tgggaattga gctcaggacc tctggaagag cagtcagtgc
     1921 tcttaacccc tgagccatct caccagcccc ctagaaacat tctattagta tctcagactt
     1981 caatatgata accattgaca ttctattaat agatgttaca tcacataatt taaaaaattg
     2041 aggaaagaaa acacaaatat ttgtacacaa tataactgat cttggcctaa ttttttaaaa
     2101 tatttactca aaatatttat atgagtacac tgtagctgtt ttcagacaca ccagaagagg
     2161 gaatcagatt ctattacaga tgcttgtgag ccaccatgtg gctgctggga attgaactca
     2221 ggacctcagg aagaacagtc agtgctctta accgctgagc catctctcca gcccttgatg
     2281 ggctaatttt aaaggccaag ggatactggt cagcttagat gtatggcctc cacagtctcc
     2341 aggatgttgg gtctcccttt agaacccatc tcaccacaac tctaagttta caaaagcagg
     2401 aacctgttac catccctttt aatcagtttc acataggaac agagaatagg cacatagggg
     2461 ccagaaaagt gggtgggaac agactgaagt tagaagccaa aggcactggc caatgagagt
     2521 gtcagcaggt gtgtgttttc ctcacccact agtttctgga ttgctttttc ttggtcaaaa
     2581 agctaacttt gtatctcttg ttttcttcac cactgctggc cagctctccc cttgcctccc
     2641 tgttgtctac ctctgtacga tgcacagtca tcagacccat tcaacgctgt tctgtcacca
     2701 cggtccttcc actgactcag aactaactct gtgtcccaca gttacttctc ataggccacc
     2761 tgcctcaagg tcattcaaga gacgcagctg acacgctgta agaaaccctt caccaactct
     2821 cagatcatat gtagaatcgg tccatttctc accgtatgat tcatctcatc ttctctggta
     2881 tcacttcgca ggagccccat cttaaatctg aaaatggccc tgactacttc ccaaactggc
     2941 tctcctgttt cagacctcca ctcctttcca agtgtttgtc tctctttaaa atgtctgagc
     3001 agctctgaat tcctggtcat tttctatctc atgctccatt ctcagctgag ggatgccttt
     3061 ccaggcaaac ccatcccagt actgagtcga agtctccact agggtgcact tgggaggctc
     3121 tctcaaagag gagtttattt ctctacctgg cttttaacta gaaagacata tagctgtgga
     3181 atgagtccaa tagctcacag acagggaaaa gcctgtcagc tctgtttctc tcctggtgtg
     3241 catgctgcgt acgtggccgg atctttcctt gttctgctaa tgagccatgc ccgcatgccg
     3301 ggcttgttta cttgcttgtt tggttaacct ttctgactct accagagtta cttccatagc
     3361 tccctagtct ttctcttaac tttggaaggg agattataga accagcctct ccgcccctag
     3421 acccgcccag ccctgtctca cttcctctcg ggaggcgggc tctcctcggg caatctctcg
     3481 ctcagttttc tggctcagat ctgagaccca tttcccgcct tcacagacct aatcaagaaa
     3541 taacttcgta tagcatacat tatacgaagt tatctcgaga tgaccgagta caagcccacg
     3601 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     3661 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     3721 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     3781 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     3841 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     3901 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     3961 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     4021 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     4081 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     4141 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagcg gccgcggaag cggagctact
     4201 aacttcagcc tgctgaagca ggctggtgac gtggaggaga accctggacc tataacttcg
     4261 tatagcatac attatacgaa gttattcacg cgtatgggca aatcatatcc tacagtgtct
     4321 gccgactatc aggacgctgt ggagaaagct aagaagaaac tgagaggctt catcgctgag
     4381 aagcggtgtg cccccctgat gctgagactg gctttccaca gcgccggaac cttcgacaag
     4441 ggaaccaaga caggcggccc tttcggaacc atcaagcacc ccgctgagct cgcccatagc
     4501 gctaacaacg gactggacat cgccgtgcgg ctgctggagc ctctgaaggc cgagttccct
     4561 atcctgagct atgccgactt ctaccagctg gctggagtgg tggccgtgga ggttaccgga
     4621 ggacctaagg tgcccttcca ccctggaaga gaggacaagc ctgagcctcc acccgaggga
     4681 cggctgcctg atcctaccaa gggaagcgac catctgcggg acgtgttcgg aaaggccatg
     4741 ggactgaccg atcaagacat cgtggccctc agcggcggac atacaatcgg agctgctcac
     4801 aaggagagaa gcggcttcga aggcccctgg acaagcaacc ccctgatctt cgacaacagc
     4861 tactttaccg aactgctcag cggcgagaag gagggactgt tgcagctgcc cagcgacaag
     4921 gctctgctca gcgaccctgt gttcagaccc ctggtggaca agtacgctgc tgacgaggat
     4981 gccttcttcg ctgactacgc cgaggctcac cagaagctga gcgagctggg cttcgctgac
     5041 gctaccggtt acccatacga tgttccagat tacgctggtg gaggcggagg ttctggggga
     5101 ggaggtagtg gcggtggtgg ttcaggaggc ggcggaagct tggatccagg tggaggtgga
     5161 agcggtagca aaggaatgga aactgaacca gtttccgtgc agaaggtacc tgcaccccct
     5221 ggatctccct gtcgacaaca ggactctgcc ctgaccccca cgcccaccat gcctccccca
     5281 gaggaaccct ccgaagacta tgaacacagc caaagccctg cagaggtact actgggttag
     5341 aaggtgtagg gctattaggt ttttttattc gtttactttg gtcgaggagt tgaggatgaa
     5401 gaaggtcgtt atagagaagt aagttaatgg gttagtgtcc taaagagaca ccaaggtcct
     5461 gaccctcctg gctgggcaga ggtagaactg atagcctaga gtaaaatagg aacaggggcc
     5521 cggcggtgtg gggcacacct ctaatcccaa cacttgggag gcagaggcag gtggatttct
     5581 gagttcaagg ccagcctggt ctctacagag tgagttccag gacagccagg gctacacaga
     5641 gaaaccctgt ctaaaaaaaa aaaaaaaaaa accgggatga gttggattgt aactggggat
     5701 agttggattg taactcctac tccctgaagc cttgcctgaa gcgcctacct cagatgaaag
     5761 actatagtcc tgtggaaggc ctggccagga gtcttatttg ctctggcctg gagctaaagg
     5821 catgtcccag acttggcaaa actatagtcg ccctccctga cccccacacc aacacttccc
     5881 gtttttcagc tgtggttgca catcccagga gcaatagtgc ttgggatggt agggctggag
     5941 ggaggatgac aagttccagg ccagcctagg ccacaaagag agttcaagac cgctgagggc
     6001 atcacagcaa gacctgtttc aaaacaaaac aaacaaacag acagaacctg gctcagacca
     6061 aagtgcctgc taagaccaac aggattcaca cagctgcttt ctcttttgat tttcatcaat
     6121 ttattttatg tgtatgaggt ttagggattt attttatttt atcttttgga acacagggtc
     6181 tcactctgta gccttggccg ggctggagct tgctttgtaa gccaggctgg tctcaaattc
     6241 atagaaacca cctgcctgcc ttctgagctc tgggattaat gtatgcgcca ccaagctcat
     6301 tttcctaagg aagcctagtt catggccttc ctgggagtat tgcctcaacc ttccatactc
     6361 accctggaag gaactacctc catcccatta ttcttcactc atttttccct tcacaaaaaa
     6421 cacaaataag gtcacattgc cctaactttt tattattagc attatatagc taaggattaa
     6481 aaatactagt tgacatttat gggtacttct ctctctctct caatctctct ctcttttttt
     6541 tttttttttg gtttttcgag acagggtttc tctgtatagc cctggctgtc ctggaactca
     6601 ctttgtgaac caggctggcc tcgaaatcgc ctgcctctgc ctcctgagtg ctgggattag
     6661 acttatgggt atttctcatg tgccaggcac catggtattg agctctttga acagtagctc
     6721 caccttctat acctatgaac taactattat tattttatat gacatgactg gaattacaca
     6781 atatttctaa ttttatggca ggcatattat ttcaccatgt cttcaaggtt catccaggtt
     6841 gttgaatgta tcaggatttc attcctcttt cagggtgaat actattttgt tacatttgca
     6901 gacacatttt gttcatgcgt tcatgtgcta aggacacttg ggtcactgtg acttttgtgg
     6961 gggtgttgct aggcacatta gtatataatt atatgcttgg atattttttc cagttctttt
     7021 gagtatatat atggaaagtg gaagcttggt catacggtag tttaagtcta actttttgag
     7081 ggtcatcctg ccaattgtgt taagcacttt atatgaagtt gatacttttc caaacttgag
     7141 tcccttaaat gcaaagatgt atctatccta tcttgtatct caggacctac tagtgagtct
     7201 aatgataagc agatgggtgc ccggtaaatt gtgcagtgag agctgcaagg ctcgaagtaa
     7261 atgaggaact ggggtgccga taagccagga catgatccaa tgactcccag gaatggaatg
     7321 tgaggtccta ccctctccaa tctccagctg aagggatggg cggccagtgg aggggacaag
     7381 acgtctgggc tttctgtagg cctcacttct ctccttggtt cactagcaag ccatacagga
     7441 ggaatttcag tttctgcgct gcccgagctg ccaggcccaa gccaagtgcc ccaaactgct
     7501 gccttgcctg cacacgctgt gctccggatg cctggaggcg cctggcctgc agtgccccat
     7561 ctgcaaggct cctgggcagg ccgatgctaa tggggaggcc ctggataacg tgttcttcga
     7621 gagcctgcag cggcgcctgg cggtgttccg gcagatcgtg gatgctcagg ctgcgtgctg
     7681 cagcccgggg gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg
     7741 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     7801 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     7861 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     7921 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     7981 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8041 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8101 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8161 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8221 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8281 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8341 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8401 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8461 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8521 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8581 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8641 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8701 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8761 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8821 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     8881 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     8941 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9001 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9061 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9121 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9181 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9241 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9301 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9361 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9421 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9481 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9541 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9601 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9661 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9721 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9781 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     9841 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     9901 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     9961 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
    10021 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
    10081 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
    10141 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
    10201 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
    10261 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
    10321 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
    10381 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
    10441 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
    10501 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcg
//
