LOCUS       sequence-296120-        2455 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2455)
  TITLE     Abbriano Lab pending publication
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CD_bsd
FEATURES             Location/Qualifiers
     source          1..2455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             25..420
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     rep_origin      complement(565..1153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(645..664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1244..2035)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gtctcgtctc gtctcaggtc tccaatggcc aagcctttgt ctcaagaaga atccaccctc
       61 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
      121 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
      181 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
      241 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
      301 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
      361 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
      421 tcaggtcgag accaccagga tacatagatt accacaactc cgagcccttc cacccacaga
      481 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
      541 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
      601 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
      661 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
      721 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
      781 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
      841 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
      901 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
      961 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1021 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1081 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1141 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1201 aaactcacgt taagggattt tggtcatgca ttctaggtga ttattatttg ccgactacct
     1261 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca
     1321 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact
     1381 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
     1441 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc
     1501 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
     1561 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
     1621 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagataccag
     1681 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
     1741 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcactct
     1801 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
     1861 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
     1921 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
     1981 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgcgaa
     2041 acgatcctca tcctgtctct tgatcagata ttgatcccct gcgccatcag atccttggcg
     2101 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag
     2161 ctggcaattc cggttcgctt gctaagcttg catgcctgca agtcgactct agaggagcgg
     2221 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
     2281 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
     2341 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
     2401 ctgtatcgag tggtgatttt gtgccgagct cattactcgc atccattctc aggct
//
