LOCUS       sequence-296125-        2626 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2626)
  TITLE     Abbriano Lab pending publication
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CD_nat
FEATURES             Location/Qualifiers
     source          1..2626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             42..608
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGFT
                     LREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNRR
                     LTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFTL
                     CGLDTALYDGTASDGEQALYMSMPCP"
     rep_origin      complement(753..1341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(833..852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1432..2223)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 gcatccattc tcaggctgtc tcgtctcgtc tcaggtctcc aatgaccact cttgacgaca
       61 cggcttaccg gtaccgcacc agtgtcccgg gggacgccga ggccatcgag gcactggatg
      121 ggtccttcac caccgacacc gtattccgcg tcaccgccac cggggacggc ttcaccctgc
      181 gggaggtgcc ggtggacccg cccctgacca aggtgttccc cgacgacgaa tcggacgacg
      241 aatcggacga cggggaggac ggcgacccgg actcccggac gttcgtcgcg tacggggacg
      301 acggcgacct ggcgggcttc gtggtcgtct cgtactccgg ctggaaccgc cggctgaccg
      361 tcgaggacat cgaggtcgcc ccggagcacc gggggcacgg ggtcgggcgc gcgttgatgg
      421 ggctcgcgac ggagttcgcc cgcgagcggg gcgccgggca cctctggctg gaggtcacca
      481 acgtcaacgc accggcgatc cacgcgtacc ggcggatggg gttcaccctc tgcggcctgg
      541 acaccgccct gtacgacggc accgcctcgg acggcgagca ggcgctctac atgagcatgc
      601 cctgcccctc aggtcgagac caccaggata catagattac cacaactccg agcccttcca
      661 cccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      721 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      781 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      841 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      901 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      961 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1021 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1081 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1141 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1201 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1261 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1321 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1381 tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtgatt attatttgcc
     1441 gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg
     1501 cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg
     1561 ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat
     1621 tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc
     1681 gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag
     1741 atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct
     1801 atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa
     1861 gataccagca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg
     1921 ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat
     1981 ctcactctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt
     2041 tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag
     2101 gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg
     2161 ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct
     2221 catgcgaaac gatcctcatc ctgtctcttg atcagatatt gatcccctgc gccatcagat
     2281 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     2341 cgccccagct ggcaattccg gttcgcttgc taagcttgca tgcctgcaag tcgactctag
     2401 aggagcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc
     2461 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg
     2521 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga
     2581 tgggctgcct gtatcgagtg gtgattttgt gccgagctca ttactc
//
