LOCUS       sequence-296180-        2836 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Origin of transfer for conjugation cargo plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2836)
  TITLE     Abbriano Lab pending publication
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CF_OriT
FEATURES             Location/Qualifiers
     source          1..2836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            151..260
                     /note="incP origin of transfer"
     CDS             293..664
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELVRINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     CDS             1239..2030
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2121..2709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2610..2629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggtggtctc gagcgatctt ccgctgcata accctgcttc ggggtcatta tagcgatttt
       61 ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga
      121 ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc
      181 gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc
      241 tgctctgcga ggctggccgg ctaccgccgg cgtaacagat gagggcaagc ggatggctga
      301 tgaaaccaag ccaaccagga agggcagccc acctatcaag gtgtactgcc ttccagacga
      361 acgaagggcg attgaggaaa aggcggcggc ggccggcatg agcctgtcgg cctacctgct
      421 ggccgtcggc cagggctaca aaatcacggg cgtcgtggac tatgagcacg tccgcgagct
      481 ggtccgcatc aatggcgacc tgggccgcct gggcggcctg ctgaaactct ggctcaccga
      541 cgacccgcgc acggcgcggt tcggtgatgc cacgatcctc gccctgctgg cgaagatcga
      601 agagaagcag gacgagcttg gcaaggtcat gatgggcgtg gtccgcccga gggcagagcc
      661 atgacttttt tagccgctaa aacggccggg gggtgcgcgt gattgccaag cacgtcccca
      721 tgcgctccat caagaagggc gacttcgcgg agctggtgaa gtacatcacc gacgagcaag
      781 gcaagacgat ccattggaga cctgagacga gacgagacag cctgagaatg gatgcgagta
      841 atgagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
      901 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
      961 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
     1021 gtaacgatga cagagcgttg ctgcctgtga tcaccgctcc tctagagtcg acttgcaggc
     1081 atgcaagctt agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga
     1141 agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat
     1201 caatatctga tcaagagaca ggatgaggat cgtttcgcat gagggaagcg gtgatcgccg
     1261 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
     1321 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
     1381 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
     1441 acgacctttt ggaaacttcg gcttcccctg gagagagtga gattctccgc gctgtagaag
     1501 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
     1561 aatttggaga atggcagcgc aatgacattc ttgctggtat cttcgagcca gccacgatcg
     1621 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
     1681 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
     1741 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
     1801 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
     1861 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
     1921 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
     1981 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa taatcaccta
     2041 gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2101 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2161 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2221 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2281 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2341 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2401 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2461 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2521 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2581 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2641 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2701 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2761 gctcacatgt tctttcctgc gttatcccct gattctgtgg gtggaagggc tcggagttgt
     2821 ggtaatctat gtatcc
//
