LOCUS       sequence-296889-        9460 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of pegRNA(s) and sgRNA(s) under control of UAS promoter. 
            NS stands for ""No Scaffold"". Can be used to generate transgenic 
            flies with vermillion+ selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9460)
  TITLE     Prime editing expression plasmids for use in Drosophila melanogaster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCFD6-NS
FEATURES             Location/Qualifiers
     source          1..9460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    147..241
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    265..359
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    1355..1785
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    1819..1888
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(2107..2125)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2146..2162)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2146..2162)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2159..2181)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2170..2186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2194..2200,2201..2218,2219..2224))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2239..2260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2377..2394)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2548..3136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2628..2647)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3307..4098,4099..4167))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3930..3949
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4168..4272)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4298..4753)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4435..4456)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4647..4666
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4880..4902
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4894..4911
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4895..4911
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4918..4937
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4918..4936
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4970..4988)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5440..5511,5913..6186,6261..6915,6977..7292,7496..7627,
                     7698..8312)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    9085..9118
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    join(9131..9460,1..101)
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
ORIGIN
        1 cccaataaaa gattattata ttgcatacct tttcttgcca taccatttag ccgatcaatt
       61 gtgctcggca acagtatatt tgtggtgtgc caaccaacaa cagatccaag ctggccgcgg
      121 ctcgatccgc ttgcatgcct gcaggtcgga gtactgtcct ccgagcggag tactgtcctc
      181 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga
      241 gcggaagctt gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg
      301 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
      361 ggagactcta gcgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg
      421 ctgagagcat cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta
      481 atatcctttg aataagccaa ctttgaatca caagacgcat accaaacgaa ttcgggcttt
      541 gagtgtgtgt agacatcaag catcggtggt tcagtggtag aatgctcgcc tgccacgcgg
      601 gcggcccggg ttcgattccc ggccgatgca gggtcttcga gaagacctaa caaagcacca
      661 gtggtctagt ggtagaatag taccctgcca cggtacagac ccgggttcga ttcccggctg
      721 gtgcatctag aggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
      781 actacctaca gagatttaaa gctctaatta aaataacaaa tcaattgttt tataatattc
      841 gtacgattct ttgattatgt aataaaatgt gatcattagg aagattacga aaaatataaa
      901 aaatatgagt tctgtgtgta taacaaatgc tgtaaacgcc acaattgtgt ttgttgcaaa
      961 taaacccatg attatttgat taaaattgtt gttttctttg ttcatagaca atagtgtgtt
     1021 ttgcctaaac gtgtactgca taaactccat gcgagtgtat agcgagctag tggctaacgc
     1081 ttgccccacc aaagtagatt cgtcaaaatc ctcaatttca tcaccctcct ccaagtttaa
     1141 catttggccg tcggaattaa cttctaaaga tgccacataa tctaataaat gaaatagaga
     1201 ttcaaacgtg gcgtcatcgt ccgtttcgac catttccgaa aagaactcgg gcataaactc
     1261 tatgatttct ctggacgtgg tgttgtcgaa actctcaaag tacgcagtca ggaacgtgcg
     1321 cgacatgtcg tcgggaaact cgcgcggaaa catttggcca cgtaataagt gtgcgttgaa
     1381 tttattcgca aaaacattgc atattttcgg caaagtaaaa ttttgttgca taccttatca
     1441 aaaaataagt gctgcatact ttttagagaa accaaataat tttttattgc atacccgttt
     1501 ttaataaaat acattgcata ccctctttta ataaaaaata ttgcatactt tgacgaaaca
     1561 aattttcgtt gcatacccaa taaaagatta ttatattgca tacccgtttt taataaaata
     1621 cattgcatac cctcttttaa taaaaaatat tgcatacgtt gacgaaacaa attttcgttg
     1681 catacccaat aaaagattat tatattgcat accttttctt gccataccat ttagccgatc
     1741 aattgtgctc ggcaacagta tatttgtggt gtgccaacca acaacggatc cactagtgtc
     1801 gacgatgtag gtcacagtct cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc
     1861 cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt cgaggtggcg
     1921 gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga aaccgctggg
     1981 cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg atacgcgggg
     2041 cagcgtcagc gggttctcga cggtcacggc gggcatgtcg acactagttc tagccagctt
     2101 ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc
     2161 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     2221 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     2281 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     2341 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     2401 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     2461 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     2521 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     2581 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2641 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2701 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     2761 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2821 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2881 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2941 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     3001 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3061 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3121 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3181 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     3241 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3301 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     3361 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     3421 tggccccagt gctgcaatga taccgcggga cccacgctca ccggctccag atttatcagc
     3481 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     3541 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     3601 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     3661 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     3721 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     3781 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3841 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3901 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3961 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4021 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4081 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4141 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4201 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     4261 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     4321 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     4381 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     4441 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     4501 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     4561 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     4621 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     4681 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     4741 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     4801 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     4861 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa
     4921 tacgactcac tatagggcga attgggtacg taccgggccc ctagtatgta tgtaagttaa
     4981 taaaacccat ttttgcggaa agtagataaa aaaaacattt ttttttttta ctgcactgga
     5041 tatcattgaa cttatctgat cagttttaaa tttacttcga tccaagggta tttgatgtac
     5101 caggttcttt cgattacctc tcactcaaaa tgacattcca ctcaaagtca gcgctgtttg
     5161 cctccttctc tgtccacaga aatatcgccg tctctttcgc cgctgcgtcc gctatctctt
     5221 tcgccaccgt ttgtagcgtt acgtagcgtc aatgtccgcc ttcagttgca ttttgtcagc
     5281 ggtttcgtga cgaagctcca agcggtttac gccatcaatt aaacacaaag tgctgtgcca
     5341 aaactcctct cgcttcttat ttttgtttgt tttttgagtg attggggtgg tgattggttt
     5401 tgggtgggta agcaggggaa agtgtgaaaa atcccggcaa tgggccaaga ggatcaggag
     5461 ctattaattc gcggaggcag caaacaccca tctgccgagc atctgaacaa tgtgagtagt
     5521 acatgtgcat acatcttaag ttcacttgat ctataggaac tgcgattgca acatcaaatt
     5581 gtctgcggcg tgagaactgc gacccacaaa aatcccaaac cgcaattgca caaacaaata
     5641 gtgacacgaa acagattatt ctggtagctg ttctcgctat ataagacaat ttttgagatc
     5701 atatcatgat caagacatct aaaggcattc attttcgact atattctttt ttacaaaaaa
     5761 tataacaacc agatatttta agctgatcct agatgcacaa aaaataaata aaagtataaa
     5821 cctacttcgt aggatacttc ggggtacttt ttgttcgggg ttagatgagc ataacgcttg
     5881 tagttgatat ttgagatccc ctatcattgc agggtgacag cggagcggct tcgcagagct
     5941 gcattaacca gggcttcggg caggccaaaa actacggcac gctccggcca cccagtccgc
     6001 cggaggactc cggttcaggg agcggccaac tagccgagaa cctcacctat gcctggcaca
     6061 atatggacat ctttggggcg gtcaatcagc cgggctccgg atggcggcag ctggtcaacc
     6121 ggacacgcgg actattctgc aacgagcgac acataccggc gcccaggaaa catttgctca
     6181 agaacggtga gtttctattc gcagtcggct gatctgtgtg aaatcttaat aaagggtcca
     6241 attaccaatt tgaaactcag tttgcggcgt ggcctatccg ggcgaacttt tggccgtgat
     6301 gggcagttcc ggtgccggaa agacgaccct gctgaatgcc cttgcctttc gatcgccgca
     6361 gggcatccaa gtatcgccat ccgggatgcg actgctcaat ggccaacctg tggacgccaa
     6421 ggagatgcag gccaggtgcg cctatgtcca gcaggatgac ctctttatcg gctccctaac
     6481 ggccagggaa cacctgattt tccaagccat ggtgcggatg ccacgacatc tgacctatcg
     6541 gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt tcgctcagca aatgtcagca
     6601 cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc ggcggagaaa ggaagcgtct
     6661 ggcattcgcc tccgaggctc taaccgatcc gccgcttctg atctgcgatg agcccacctc
     6721 cggactggac tcctttaccg cccacagcgt cgtccaggtg ctgaagaagc tgtcgcagaa
     6781 gggcaagacc gtcatcctga ccattcatca gccgtcatcc gagctgtttg agctctttga
     6841 caagatcctt ctgatggccg agggcagggt agctttcttg ggcactccca gcgaagccgt
     6901 cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg tatctagtat tacataacat
     6961 ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa ctacaatccg gcggactttt
     7021 acgtacaggt gttggccgtt gtgcccggac gggagatcga gtcccgtgat cggatcgcca
     7081 agatatgcga caattttgcc attagcaaag tagcccggga tatggagcag ttgttggcca
     7141 ccaaaaatct ggagaagcca ctggagcagc cggagaatgg gtacacctac aaggccacct
     7201 ggttcatgca gttccgggcg gtcctgtggc gatcctggct gtcggtgctc aaggaaccac
     7261 tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg ttccagtgga aacaaatgat
     7321 ataacgctta caattcttgg aaacaaattc gctagatttt agatagaatt gcctgattcc
     7381 acacccttct tagttttttt caatgagatg tatagtttat agttttgcag aagataaata
     7441 aatttcattt aactcgcgaa tattaatgag atgcgagtaa cattttaatt tgcagatggt
     7501 tgccatcttg attggcctca tctttttggg ccaacaactc acgcaagtgg gtgtgatgaa
     7561 tatcaacgga gccatcttcc tcttcctgac caacatgacc tttcaaaacg tctttgccac
     7621 gataaatgta agtcatgttt agaatacatt tgcatttcaa taatttacta actttctaat
     7681 gaatcgattc gatttaggtg ttcacctcag agctgccagt ttttatgagg gaggcccgaa
     7741 gtcgacttta tcgctgtgac acatactttc tgggcaaaac gattgccgaa ttgccgcttt
     7801 ttctcacagt gccactggtt ttcacggcga ttgcctatcc gatgatcgga ctgcgggccg
     7861 gagtgctgca cttcttcaac tgcctggcgc tggtcactct ggtggccaat gtgtcaacgt
     7921 ccttcggata tctaatatcc tgcgccagct cctcgacctc gatggcgctg tctgtgggtc
     7981 cgccggttat cataccattc ctgctctttg gcggcttctt cttgaactcg ggctcggtgc
     8041 cagtatacct caaatggttg tcgtacctct catggttccg ttacgccaac gagggtctgc
     8101 tgattaacca atgggcggac gtggagccgg gcgaaattag ctgcacatcg tcgaacacca
     8161 cgtgccccag ttcgggcaag gtcatcctgg agacgcttaa cttctccgcc gccgatctgc
     8221 cgctggacta cgtgggtctg gccattctca tcgtgagctt ccgggtgctc gcatatctgg
     8281 ctctaagact tcgggcccga cgcaaggagt agccgacata tatccgaaat aactgcttgt
     8341 tttttttttt accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat
     8401 gtaattatat ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct
     8461 tcgaacatcc ccacaagtag actttggatt tgtttctaac caaaagactt acacacctgc
     8521 ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata ttttttatat
     8581 acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc acgtttcgta
     8641 gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt gttcgacgac
     8701 cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc tcaaatggtt ccgagtggtt
     8761 catttcgtct caatagaaat tagtaataaa tatttgtatg tacaatttat ttgctccaat
     8821 atatttgtat atatttccct cacagctata tttattctaa tttaatatta tgacttttta
     8881 aggtaatttt ttgtgacctg ttcggagtga ttagcgttac aatttgaact gaaagtgaca
     8941 tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt
     9001 ttatatatat caaaaataac aactataata ataagaatac atttaattta gaaaatgctt
     9061 ggatttcact ggaactaggc tagcataact tcgtataatg tatgctatac gaagttatgc
     9121 tagcggatcc tggccacgta ataagtgtgc gttgaattta ttcgcaaaaa cattgcatat
     9181 tttcggcaaa gtaaaatttt gttgcatacc ttatcaaaaa ataagtgctg catacttttt
     9241 agagaaacca aataattttt tattgcatac ccgtttttaa taaaatacat tgcataccct
     9301 cttttaataa aaaatattgc atactttgac gaaacaaatt ttcgttgcat acccaataaa
     9361 agattattat attgcatacc cgtttttaat aaaatacatt gcataccctc ttttaataaa
     9421 aaatattgca tacgttgacg aaacaaattt tcgttgcata
//
