LOCUS       sequence-297020-        6014 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Bait vector PXC2 X021_pECIA2 should be used with prey vector PXC2 
            X021_pECIA14..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: PXC2 X021_pECIA2
FEATURES             Location/Qualifiers
     source          1..6014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        23..449
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     467..520
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             2267..2290
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2996..3037
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3047..3064
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3087..3104)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3137..3271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3161..3180)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3215..3234
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3415..3431)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3415..3431)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3428..3450)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3439..3455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3463..3469,3470..3487,3488..3493))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3508..3529
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3646..3663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3817..4405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3897..3916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4576..5367,5368..5436))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5199..5218
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5437..5541)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5609..5627
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5665..5687)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5787..5806
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(6000..6014,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttaattcgtt gcaggacagg atgtggtgcc cgatgtgact
       61 agctctttgc tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc
      121 ccccaccgcc caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat
      181 gccccatgtg ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga
      241 accaattctt cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc
      301 ggccggcgtg tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc
      361 aaaatgtata aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
      421 caagtgaatc atctcagtgc aactaaaggg gggatccgat ctcaatatga agttatgcat
      481 attactggcc gtcgtggcct ttgttggcct ctcgctcggg acattcaacg acgacgtttt
      541 gggtctcatc gttttcaagg ccggactcga cgaccctctc tctaagctct cttcttggaa
      601 ctcggaagac tacgatccct gcaactgggt cggttgcaca tgcgaccctg ccaccaatcg
      661 ggtctcggag ctccgccttg acgccttctc tctctccggt cacatcggcc gtggccttct
      721 ccgtctccag ttcctccaca ccttggtcct ctccaacaac aacctcaccg gaaccctaaa
      781 ccccgagttc cctcacctcg gcagcctcca agtcgtcgat ttcagtggca ataatctctc
      841 gggtcggatc ccagacgggt tcttcgagca atgcgggtcc ttgaggtccg tttccttggc
      901 taacaacaaa ctcacaggtt ctattcctgt ctccctcagc tactgttcta ctctcaccca
      961 tcttaacctc tcaagcaatc aactctctgg gaggttgccc cgagatatct ggttcctcaa
     1021 gagcttaaag tcccttgatt tctctcacaa tttcctccaa ggagatatcc ccgatggcct
     1081 cggcggtctc tacgatctcc gtcatatcaa cttatccagg aactggtttt ccggtgacgt
     1141 tccttccgac atcggacgat gctcttccct taaatcgctt gacttgagtg agaactactt
     1201 ctccgggaat cttccggatt cgatgaaaag cctgggctct tgcagctcca tccgtctgcg
     1261 aggaaactct ctgatcggag aaatccctga ttggatcggc gacattgcca ctctcgagat
     1321 tcttgatctc tccgccaaca acttcaccgg cactgtccca ttctccctcg gaaatctcga
     1381 gtttttgaag gatctcaatc tatccgcaaa catgctcgct ggagagctcc cacagactct
     1441 ctccaactgc tccaacctaa tctccattga tgtcagcaag aattcattca ccggagatgt
     1501 gcttaagtgg atgttcaccg gaaactctga aagttcctcg ctttccagat ttagcctcca
     1561 caaaagatca gggaacgaca ccattatgcc catcgttgga ttcctccaag gtctccgggt
     1621 cctcgatctg tcttcaaacg gcttcaccgg tgaattaccg tctaacattt ggattctcac
     1681 aagtttgttg cagctcaata tgtccaccaa ctctctcttc ggttccatac ccaccggcat
     1741 tggaggattg aaagtcgctg agattcttga tcttagtagt aatcttctca acggaaccct
     1801 tccttctgag atcggaggag ccgtctccct gaaacaactt catctccaca gaaaccgcct
     1861 ctccggccag attccggcaa agatctctaa ctgttcggct ttaaacacaa taaatctatc
     1921 ggagaatgaa ctatccggtg cgattccggg gagcattggc agtctgagca accttgagta
     1981 cattgatctg tcgagaaaca atctctcggg aagtcttccc aaagaaatag agaaactttc
     2041 gcatctcttg acctttaaca tctcccacaa caacatcaca ggggagctgc cagctggagg
     2101 tttcttcaac accatccctc tctctgctgt caccggaaac ccatcccttt gtggctccgt
     2161 cgttaaccgc tcatgtctct ctgtccaccc caaaccaatc gtcctcaacc ccaactcctc
     2221 caatccaaca aatggtcctg ctctcacagg gcagatcagg aagagcctgg aggtgctgtt
     2281 ccagggacca gggagcgaca aaactcacac atgcccaccg tgcccagcac ctgaactcct
     2341 ggggggaccg tcagtcttcc tcttcccccc aaaacccaag gacaccctca tgatctcccg
     2401 gacccctgag gtcacatgcg tggtggtgga cgtgagccac gaagaccctg aggtcaagtt
     2461 caactggtac gtggacggcg tggaggtgca taatgccaag acaaagccgc gggaggagca
     2521 gtacaacagc acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa
     2581 tggcaaggag tacaagtgca aggtctccaa caaagccctc ccagccccca tcgagaaaac
     2641 catctccaaa gccaaagggc agccccgaga accacaggtg tacaccctgc ccccatcccg
     2701 ggaggagatg accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctatcccag
     2761 cgacatcgcc gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc
     2821 tcccgtgctg gactccgacg gctccttctt cctctacagc aagctcaccg tggacaagag
     2881 caggtggcag caggggaacg tcttctcatg ctccgtgatg catgagggtc tgcacaacca
     2941 ctacacgcag aagagcctct ccctgtctcc gggtaaatct agagggccct tcgaaggtaa
     3001 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca
     3061 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctaaggcct gagctcgctg
     3121 atcagcctcg atcgaggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     3181 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     3241 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     3301 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     3361 tatggctgat tatgatcagt cgacctgcag gcatgcaagc ttggcgtaat catggtcata
     3421 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3481 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3541 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3601 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3661 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3721 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3781 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3841 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3901 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3961 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4021 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4081 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4141 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4201 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4261 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4321 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4381 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4441 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4501 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4561 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4621 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4681 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4741 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4801 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4861 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4921 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4981 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     5041 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     5101 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5161 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5221 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5281 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5341 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5401 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5461 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5521 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     5581 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     5641 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     5701 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     5761 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     5821 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     5881 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     5941 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     6001 ccagtcacga cgtt
//
