LOCUS       sequence-297589-        4317 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF7b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4317)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4136070
FEATURES             Location/Qualifiers
     source          1..4317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    186..307
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(197..216)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     251..270
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      422..468
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      498..634
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    679..750
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(976..1564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1056..1075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2310..2378,2379..3170)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2528..2547)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3183..3319
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3471..3542
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3547..3593)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3611..3659
                     /note="synthetic polyadenylation signal"
     enhancer        3740..4043
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4045..4247
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4198..4218
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 caccatgata gaactatcgc tcatcgactt ttatctgtgc ttcttggctt tcctgctttt
       61 tctggtgctc attatgctaa tcattttttg gttcagtctg gagttacaag atcataatga
      121 gacatgtcac gcctaagcta gcttgactga ctgagataca gcgtaccttc agctcacaga
      181 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      241 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
      301 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
      361 ggttttttaa agcaagtaaa acctctacaa atgtggtatt ggcccatctc tatcggtatc
      421 gtagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtg cccctcgggc
      481 cggattgcta tctaccggca ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct
      541 tatactccca catatgccag attcagcaac ggatacggct tccccaactt gcccacttcc
      601 atacgtgtcc tccttaccag aaatttatcc ttaaggtcgt cagctatcct gcaggcgatc
      661 tctcgatttc gatcaagaca ttcctttaat ggtcttttct ggacaccact aggggtcaga
      721 agtagttcat caaactttct tccctcccta atctcattgg ttaccttggg ctatcgaaac
      781 ttaattaacc agtcaagtca gctacttggc gagatcgact tgtctgggtt tcgactacgc
      841 tcagaattgc gtcagtcaag ttcgatctgg tccttgctat tgcacccgtt ctccgattac
      901 gagtttcatt taaatcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      961 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     1021 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1081 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1141 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1201 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1261 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1321 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1381 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1441 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1501 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1561 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1621 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1681 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     1741 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1801 atttaaattt ccgaactctc caaggccctc gtcggaaaat cttcaaacct ttcgtccgat
     1861 ccatcttgca ggctacctct cgaacgaact atcgcaagtc tcttggccgg ccttgcgcct
     1921 tggctattgc ttggcagcgc ctatcgccag gtattactcc aatcccgaat atccgagatc
     1981 gggatcaccc gagagaagtt caacctacat cctcaatccc gatctatccg agatccgagg
     2041 aatatcgaaa tcggggcgcg cctggtgtac cgagaacgat cctctcagtg cgagtctcga
     2101 cgatccatat cgttgcttgg cagtcagcca gtcggaatcc agcttgggac ccaggaagtc
     2161 caatcgtcag atattgtact caagcctggt cacggcagcg taccgatctg tttaaaccta
     2221 gatattgata gtctgatcgg tcaacgtata atcgagtcct agcttttgca aacatctatc
     2281 aagagacagg atcagcagga ggctttcgca tgagtattca acatttccgt gtcgccctta
     2341 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2401 taaaagatgc tgaagatcag ttgggtgcgc gagtgggtta catcgaactg gatctcaaca
     2461 gcggtaagat ccttgagagt tttcgccccg aagaacgctt tccaatgatg agcactttta
     2521 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2581 gccgcataca ctattctcag aatgacttgg ttgagtattc accagtcaca gaaaagcatc
     2641 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2701 ctgcggccaa cttacttctg acaacgattg gaggaccgaa ggagctaacc gcttttttgc
     2761 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2821 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacc ttgcgtaaac
     2881 tattaactgg cgaactactt actctagctt cccggcaaca gttgatagac tggatggagg
     2941 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3001 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3061 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3121 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ccgattctag
     3181 gtgcattggc gcagaaaaaa atgcctgatg cgacgctgcg cgtcttatac tcccacatat
     3241 gccagattca gcaacggata cggcttcccc aacttgccca cttccatacg tgtcctcctt
     3301 accagaaatt tatccttaag atcccgaatc gtttaaactc gactctggct ctatcgaatc
     3361 tccgtcgttt cgagcttacg cgaacagccg tggcgctcat ttgctcgtcg ggcatcgaat
     3421 ctcgtcagct atcgtcagct tacctttttg gcagcgatcg cggctcccga catcttggac
     3481 cattagctcc acaggtatct tcttccctct agtggtcata acagcagctt cagctacctc
     3541 tcaattcaaa aaacccctca agacccgttt agaggcccca aggggttatg ctatcaatcg
     3601 ttgcgttaca cacacaaaaa accaacacac atccatcttc gatggatagc gattttatta
     3661 tctaactgct gatcgagtgt agccagatct agtaatcaat tacggggtca ttagttcata
     3721 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     3781 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     3841 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     3901 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     3961 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     4021 tattagtcat cgctattacc atgctgatgc ggttttggca gtacatcaat gggcgtggat
     4081 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     4141 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     4201 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
     4261 gtcagatcag atctttgtcg atcctaccat ccactcgaca cacccgccag cggccgc
//
