LOCUS       sequence-297612-        4371 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF6 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4371)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4136065
FEATURES             Location/Qualifiers
     source          1..4371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    240..361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(251..270)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     305..324
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      476..522
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      552..688
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    733..804
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(1030..1618)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1110..1129)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(2364..2432,2433..3224)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2582..2601)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      3237..3373
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3525..3596
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3601..3647)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3665..3713
                     /note="synthetic polyadenylation signal"
     enhancer        3794..4097
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4099..4301
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4252..4272
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 caccatgttt catctcgttg actttcaggt tactatagca gagatattac taattattat
       61 gaggactttt aaagtttcca tttggaatct tgattacatc ataaacctca taattaaaaa
      121 tttatctaag tcactaactg agaataaata ttctcaatta gatgaagagc aaccaatgga
      181 gattgattaa gctagcttga ctgactgaga tacagcgtac cttcagctca cagacatgat
      241 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
      301 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
      361 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
      421 ttaaagcaag taaaacctct acaaatgtgg tattggccca tctctatcgg tatcgtagca
      481 taaccccttg gggcctctaa acgggtcttg aggggttttt tgtgcccctc gggccggatt
      541 gctatctacc ggcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact
      601 cccacatatg ccagattcag caacggatac ggcttcccca acttgcccac ttccatacgt
      661 gtcctcctta ccagaaattt atccttaagg tcgtcagcta tcctgcaggc gatctctcga
      721 tttcgatcaa gacattcctt taatggtctt ttctggacac cactaggggt cagaagtagt
      781 tcatcaaact ttcttccctc cctaatctca ttggttacct tgggctatcg aaacttaatt
      841 aaccagtcaa gtcagctact tggcgagatc gacttgtctg ggtttcgact acgctcagaa
      901 ttgcgtcagt caagttcgat ctggtccttg ctattgcacc cgttctccga ttacgagttt
      961 catttaaatc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     1021 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     1081 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1141 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1201 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     1261 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     1321 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     1381 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1441 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1501 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1561 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1621 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     1681 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     1741 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     1801 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcatttaa
     1861 atttccgaac tctccaaggc cctcgtcgga aaatcttcaa acctttcgtc cgatccatct
     1921 tgcaggctac ctctcgaacg aactatcgca agtctcttgg ccggccttgc gccttggcta
     1981 ttgcttggca gcgcctatcg ccaggtatta ctccaatccc gaatatccga gatcgggatc
     2041 acccgagaga agttcaacct acatcctcaa tcccgatcta tccgagatcc gaggaatatc
     2101 gaaatcgggg cgcgcctggt gtaccgagaa cgatcctctc agtgcgagtc tcgacgatcc
     2161 atatcgttgc ttggcagtca gccagtcgga atccagcttg ggacccagga agtccaatcg
     2221 tcagatattg tactcaagcc tggtcacggc agcgtaccga tctgtttaaa cctagatatt
     2281 gatagtctga tcggtcaacg tataatcgag tcctagcttt tgcaaacatc tatcaagaga
     2341 caggatcagc aggaggcttt cgcatgagta ttcaacattt ccgtgtcgcc cttattccct
     2401 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2461 atgctgaaga tcagttgggt gcgcgagtgg gttacatcga actggatctc aacagcggta
     2521 agatccttga gagttttcgc cccgaagaac gctttccaat gatgagcact tttaaagttc
     2581 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2641 tacactattc tcagaatgac ttggttgagt attcaccagt cacagaaaag catcttacgg
     2701 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2761 ccaacttact tctgacaacg attggaggac cgaaggagct aaccgctttt ttgcacaaca
     2821 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2881 acgacgagcg tgacaccacg atgcctgtag caatggcaac aaccttgcgt aaactattaa
     2941 ctggcgaact acttactcta gcttcccggc aacagttgat agactggatg gaggcggata
     3001 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     3061 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     3121 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     3181 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaaccgatt ctaggtgcat
     3241 tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac atatgccaga
     3301 ttcagcaacg gatacggctt ccccaacttg cccacttcca tacgtgtcct ccttaccaga
     3361 aatttatcct taagatcccg aatcgtttaa actcgactct ggctctatcg aatctccgtc
     3421 gtttcgagct tacgcgaaca gccgtggcgc tcatttgctc gtcgggcatc gaatctcgtc
     3481 agctatcgtc agcttacctt tttggcagcg atcgcggctc ccgacatctt ggaccattag
     3541 ctccacaggt atcttcttcc ctctagtggt cataacagca gcttcagcta cctctcaatt
     3601 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctatca atcgttgcgt
     3661 tacacacaca aaaaaccaac acacatccat cttcgatgga tagcgatttt attatctaac
     3721 tgctgatcga gtgtagccag atctagtaat caattacggg gtcattagtt catagcccat
     3781 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3841 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3901 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3961 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     4021 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     4081 tcatcgctat taccatgctg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     4141 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     4201 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     4261 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
     4321 tcagatcttt gtcgatccta ccatccactc gacacacccg ccagcggccg c
//
