LOCUS       sequence-297822-       11684 bp    DNA     circular SYN 15-APR-2025
DEFINITION  mApple expression regulated by the CaMV35S promoter, mCitrine 
            expression regulated by the A. thaliana MYB60 promoter. 
            nourseothricin acetyl transferase selectable marker (nat).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11684)
  TITLE     Quantitative comparison of specialised leaf promoters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GS1.1/MYB60
FEATURES             Location/Qualifiers
     source          1..11684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             453..1163
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     866..885
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(1166..1190)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1510..1534
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2865..3494
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3923..4996
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5062..5256
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5492..5514)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5600..5740
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5655..5674
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5755..5772)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5926..6514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6006..6025)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6760..7551)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8072..8096
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(8189..8441)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8464..9036)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(9078..9257)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      complement(9367..9619)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    9622..9646
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(9661..10374,10375..10377,10378..10380))
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9706..9727)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     10055..10074
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10314..10335
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(11014..11038)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ccctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggactccgg tatttttaca
      361 acaattacca caacaaaaca aacaacaaac aacattacaa tttactattc tagtcgacct
      421 gcaggcggcc gcactagtga tatcacaagt ttatggtgag caagggcgag gagaataaca
      481 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
      541 acgagttcga gatcgagggc gagggcgagg gccgccccta cgaggccttt cagaccgcta
      601 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
      661 tcatgtacgg ctccaaggtc tacattaagc acccagccga catccccgac tacttcaagc
      721 tgtccttccc cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta
      781 ttcacgttaa ccaggactcc tccctgcagg acggcgtgtt catctacaag gtgaagctgc
      841 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      901 cctccgagga gcggatgtac cccgaggacg gcgccctgaa gagcgagatc aagaagaggc
      961 tgaagctgaa ggacggcggc cactacgccg ccgaggtcaa gaccacctac aaggccaaga
     1021 agcccgtgca gctgcccggc gcctacatcg tcgacatcaa gttggacatc gtgtcccaca
     1081 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     1141 gcatggacga gctgtacaag taatcgttca gctttcttgt acaaagtggt gatatcccgc
     1201 ggccatgcta gagtccgcaa aaatcaccag tctctctcta caaatctatc tctctctatt
     1261 tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt
     1321 tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt
     1381 ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacctgcagg catgcgacgt
     1441 cgggcccaag cttagcttga gcttggatca gattgtcgtt tcccgccttc agtttaaact
     1501 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     1561 taatcggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg
     1621 ccaaccacag ggttcccctc gggatcaaag tactttaaag tactttaaag tactttaaag
     1681 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
     1741 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
     1801 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
     1861 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
     1921 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
     1981 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
     2041 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     2101 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     2161 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     2221 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     2281 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     2341 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     2401 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     2461 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     2521 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     2581 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     2641 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     2701 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     2761 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     2821 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     2881 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     2941 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     3001 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     3061 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     3121 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     3181 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     3241 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     3301 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     3361 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     3421 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     3481 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     3541 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     3601 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     3661 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     3721 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     3781 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     3841 cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa
     3901 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     3961 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     4021 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     4081 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     4141 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     4201 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     4261 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     4321 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     4381 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     4441 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     4501 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     4561 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     4621 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     4681 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     4741 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     4801 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     4861 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     4921 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     4981 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     5041 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     5101 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     5161 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     5221 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     5281 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     5341 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     5401 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     5461 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     5521 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     5581 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     5641 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     5701 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     5761 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5941 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6001 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6061 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6121 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6181 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6241 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6301 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6361 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6421 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6481 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6541 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc atgatatatc
     6601 tcccaatttg tgtagggctt attatgcacg cttaaaaata ataaaagcag acttgacctg
     6661 atagtttggc tgtgagcaat tatgtgctta gtgcatctaa cgcttgagtt aagccgcgcc
     6721 gcgaagcggc gtcggcttga acgaatttct agctagacat tatttgccga ctaccttggt
     6781 gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg
     6841 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
     6901 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac
     6961 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
     7021 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
     7081 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
     7141 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
     7201 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
     7261 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
     7321 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
     7381 ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac
     7441 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
     7501 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccccca tgatgtttaa
     7561 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca
     7621 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa
     7681 aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt
     7741 caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagct tacgaaccga
     7801 acaggcttat gtccactggg ttcgtgcccg aattgatcac aggcagcaac gctctgtcat
     7861 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
     7921 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
     7981 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
     8041 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
     8101 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg
     8161 aattgaatta tcagcttgca tgccggtcga tctagtaaca tagatgacac cgcgcgcgat
     8221 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
     8281 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
     8341 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
     8401 ttcaatctta agaaacttta ttgccaaatg tttgaacgat ctgcttgact ctaggggtca
     8461 tcattagggg cagggcatgc tcatgtagag cgcctgctcg ccgtccgagg cggtgccgtc
     8521 gtacagggcg gtgtccaggc cgcagagggt gaaccccatc cgccggtacg cgtggatcgc
     8581 cggtgcgttg acgttggtga cctccagcca gaggtgcccg gcaccccgct cgcgggcgaa
     8641 ctccgtcgcg agccccatca acgcgcgccc gaccccgtgc ccccggtgct ccggggcgac
     8701 ctcgatgtcc tcgacggtca gccggcggtt ccagccggag tacgagacga ccacgaagcc
     8761 cgccaggtcg ccgtcgtccc cgtacgcgac gaacgtccga gagtccgggt cgccgtcctc
     8821 cccgtcgtcc gattcgtcgt ccgattcgtc gtcggggaac accttggtca ggggcgggtc
     8881 caccggcacc tcccgcaggg tgaagccgtc cccggtggcg gtgacgcgga agacggtgtc
     8941 ggtggtgaag gacccatcca gtgcctcgat ggcctcggcg tcccccggga cactggtgcg
     9001 gtaccggtaa gccgtgtcgt caagagtggt acccatggta gatcccctcg atcgagttga
     9061 gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc agtggagcat
     9121 ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga acgcgcaata
     9181 atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc tcagtggctc
     9241 cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg
     9301 ggggtcataa cgtgactccc ttaattctcc gctcatgatc gataattcga gctctcccat
     9361 atggtcgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct
     9421 atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc
     9481 atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca
     9541 gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt
     9601 gccaaatgtt tgaacgatct gccaactttg tacaagaaag ctgggtcggc gcgcccaccc
     9661 ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
     9721 gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggt agctcaggta
     9781 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
     9841 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc
     9901 gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt
     9961 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
    10021 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
    10081 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
    10141 cttcatgtgg tcggggtagc gggcgaagca catcaggccg tagccgaagg tggtcacgag
    10201 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
    10261 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
    10321 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
    10381 ctttctctct ctctcttcct ctagatctct ctgagtctta tgctctcttt atagatgtga
    10441 gaatatgtat gtatatgagt taaatgcaac atgtatgtac atatatgtga aattaatggg
    10501 tgacttaaag aagagggaag aacttttaaa tctgcttttt cttgacaacc tcttttggat
    10561 ctgccaagct cacgagtcac tctgcctttt ccattaacta ttttgcgtag tcaattaacg
    10621 aagatatgtt gatctccaca cgaatatgta tatctccctt aagaaaacgt tactggatta
    10681 tttatttagt gaacgatcca tcaaatgttt tttcaatcga aatatttcca taaaaggagc
    10741 tcgttaacat attgtagatg aatacatagt gtatcgatta caatattttg cttaatttgg
    10801 aacattcgtt ggtaattact tataaatata gtgataatca actcataaat ggaagaagag
    10861 tcacaagcta tgttacttac atttttgtga taatagttgt aattgtggta agaaaataaa
    10921 taaagataaa gtgtgggaag aaacacaatt atttcattga atttctccat gattaaaggg
    10981 aatcattcac tgcaacttgg ggggcggccg cggagcctgc ttttttgtac aaagttggca
    11041 ttataaaaaa gcattgctca tcaatttgtt gcaactagag ccaagctgat ctcctttgcc
    11101 ccggagatca ccatggacga ctttctctat ctctacgatc taggaagaaa gttcgacgga
    11161 gaaggtgacg ataccatgtt caccaccgat aatgagaaga ttagcctctt caatttcaga
    11221 aagaatgctg acccacagat ggttagagag gcctacgcgg caggtctcat caagacgatc
    11281 tacccgagta ataatctcca ggagatcaaa taccttccca agaaggttaa agatgcagtc
    11341 aaaagattca ggactaactg catcaagaac acagagaaag atatatttct caagatcaga
    11401 agtactattc cagtatggac gattcaaggc ttgcttcata aaccaaggca agtaatagag
    11461 attggagtct ctaagaaagt agttcctact gaatcaaagg ccatggagtc aaaaattcag
    11521 atcgaggatc taacagaact cgccgtgaag actggcgaac agttcataca gagtctttta
    11581 cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac tctcgtctac
    11641 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctat
//
