LOCUS       sequence-298435-       12160 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Independent expression of QuasAr and human Kvbeta 1 in pFCK vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12160)
  AUTHORS   Cho IH, Panzera LC, Chin M, Alpizar SA, Olveda GE, Hill RA, Hoppa MB
  TITLE     The potassium channel subunit K(v)beta1 serves as a major control 
            point for synaptic facilitation.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Nov 24;117(47):29937-29947. doi: 
            10.1073/pnas.2000790117. Epub 2020 Nov 9.
  PUBMED    33168717
REFERENCE   2  (bases 1 to 12160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.2000790117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-11-24- 24"; volume: "117"; issue: "47"; pages: 
            "29937-29947"
COMMENT     Sequence Label: QuasAr-P2A-hKvb1
FEATURES             Location/Qualifiers
     source          1..12160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3890
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             3903..4661
                     /codon_start=1
                     /product="achaerhodopsin-3 (Arch) derivative with improved 
                     voltage sensitivity (Hochbaum et al., 2014)"
                     /label=QuasAr2
                     /translation="MVSIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVSGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLL
                     LHLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGAD"
     CDS             4668..5375
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEYNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             5472..5528
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6750..6779
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6798..6821
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6852..7440
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6905..6925)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7323..7334)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7443..7459)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7444..7460)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7965..8145
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8171..8188)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8177..8401
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8447..8875
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8534..8553)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8744..8765
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8884..8904)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8889..9218
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9069..9204
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9131..9150
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9266..9313
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9332..9706
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9836..9957
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9873..9892)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9927..9946
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10006..10022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10006..10022)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10019..10041)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10030..10046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10054..10060,10061..10078,10079..10084))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10099..10120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10237..10254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10408..10996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10488..10507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11167..11958,11959..12027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11790..11809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12028..12132)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt
     2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag
     2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat
     2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca
     2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg
     2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga
     2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg
     3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga
     3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac
     3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg
     3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa
     3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt
     3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga
     3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc
     3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa
     3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag
     3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac
     3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag
     3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac
     3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg
     3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt
     3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatcca
     3901 ccatggtaag tatcgctctg caggctggtt acgacctact gggtgacggc agacctgaaa
     3961 ccctgtggct gggcatcggc actctgctga tgctgattgg aaccttctac tttctggtcc
     4021 gcggatgggg agtcaccgat aaggatgccc gggaatatta cgctgtgact atcctggtgt
     4081 ccggaatcgc atccgccgca tatctgtcta tgttctttgg tatcgggctt actgaggtgt
     4141 ccgtcggggg cgaaatgttg gatatctatt atgccaggta cgcccagtgg ctgtttacca
     4201 ccccacttct gctgctgcac ctggcccttc tcgctaaggt ggatcgggtg accatcggca
     4261 ccctggtggg tgtggacgcc ctgatgatcg tcactggcct catcggagcc ttgagccaca
     4321 cggccatagc cagatacagt tggtggttgt tctctacaat ttgcatgata gtggtgctct
     4381 atgttctggc tacatccctg cgatctgctg caaaggagcg gggccccgag gtggcatcta
     4441 cctttaacac cctgacagct ctggtcttgg tgctgtggac cgcttaccct atcctgtgga
     4501 tcataggcac tgagggcgct ggcgtggtgg gcctgggcat cgaaactctg ctgtttatgg
     4561 tgttggacgt gactgccaag gtcggctttg gctttatcct gttgagatcc cgggctattc
     4621 tgggcgacac cgaggcacca gaacccagtg ccggtgccga tacgttgatg gtgagcaagg
     4681 gcgaggagta taacatggcc atcatcaagg agttcatgcg cttcaaggtg cgcatggagg
     4741 gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     4801 gctttcagac cgctaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca
     4861 tcctgtcccc tcatttcacc tacggctcca aggcctacgt gaagcacccc gccgacatcc
     4921 ccgactactt caagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaactacg
     4981 aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct
     5041 acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtgatg cagaagaaga
     5101 ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggtgcc ctgaagggca
     5161 agatcaagat gaggctgaag ctgaaggacg gcggccacta cacctccgag gtcaagacca
     5221 cctacaaggc caagaagccc gtgcagctgc ccggcgccta catcgtcgac atcaagttgg
     5281 acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc
     5341 gccactccac cggcggcatg gacgagctgt acaagagtag aatcacaagc gaaggcgagt
     5401 acatccccct ggatcaaata gacataaatg taggtggatt ttgttatgag aatgaagaat
     5461 tcggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc
     5521 ctggacctat gcaagtctcc atagcctgca cagagcacaa tttgaagagt cggaatggtg
     5581 aggaccgact tctgagcaag cagagctcca ccgcccccaa tgtggtgaac gcagcccggg
     5641 ccaaattccg cacggtcgct atcatcgcgc gcagcctggg gacgttcacg cctcagcatc
     5701 acatttctct caaagagtcc accgcaaagc agactggcat gaaatatagg aatcttggaa
     5761 aatcaggact cagagtttct tgcttgggtc ttggaacatg ggtgacattt ggaggtcaaa
     5821 tttcagatga ggttgctgaa cggctgatga ccatcgccta tgaaagtggt gttaacctct
     5881 ttgatactgc cgaagtctat gctgctggaa aggctgaagt gattctgggg agcatcatca
     5941 agaagaaagg ctggaggagg tccagtctgg tcataacaac caaactctac tggggtggaa
     6001 aagctgaaac agaaagaggg ctgtcaagaa agcatattat tgaaggattg aagggctccc
     6061 tccagaggct gcagctcgag tatgtggatg tggtctttgc aaatcgaccg gacagtaaca
     6121 ctcccatgga agaaattgtc cgagccatga cacatgtgat aaaccaaggc atggcgatgt
     6181 actggggcac ctcgagatgg agtgctatgg agatcatgga agcctattct gtagcaagac
     6241 agttcaatat gatcccaccg gtctgtgaac aagctgagta ccatcttttc cagagagaga
     6301 aagtggaggt ccagctgcca gagctctacc acaaaatagg tgttggcgca atgacatggt
     6361 ctccacttgc ctgtggaatc atctcaggaa aatacggaaa cggggtgcct gaaagttcca
     6421 gggcttcact gaagtgctac cagtggttga aagaaagaat tgtaagtgaa gaagggagaa
     6481 aacagcaaaa caagctaaaa gacctttccc caattgcgga gcgtctggga tgcacactac
     6541 ctcagctagc tgttgcgtgg tgcctgagaa atgaaggtgt gagttctgtg ctcctgggat
     6601 catccactcc tgaacaactc attgaaaacc ttggtgccat tcaggttctc ccaaagatga
     6661 catcacatgt ggtaaatgag attgataaca tactgcgcaa caagccctac agcaagaagg
     6721 actatagatc aacgcgtacg cggccgctcg agcagaaact catctcagaa gaggatctgg
     6781 cagcaaatga tatcctggat tacaaggatg acgacgataa ggtttaagaa ttcgatatca
     6841 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6901 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6961 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7021 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7081 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7141 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7201 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7261 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7321 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7381 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7441 atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     7501 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     7561 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7621 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     7681 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     7741 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     7801 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     7861 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     7921 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     7981 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8041 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8101 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc
     8161 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     8221 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     8281 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     8341 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     8401 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     8461 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     8521 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     8581 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     8641 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     8701 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     8761 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     8821 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     8881 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     8941 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     9001 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     9061 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     9121 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     9181 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     9241 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     9301 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     9361 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     9421 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     9481 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     9541 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     9601 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     9661 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     9721 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     9781 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     9841 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9901 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     9961 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
    10021 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    10081 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    10141 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    10201 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    10261 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    10321 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    10381 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    10441 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    10501 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    10561 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    10621 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    10681 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    10741 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    10801 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
    10861 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    10921 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    10981 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    11041 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    11101 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    11161 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    11221 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    11281 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    11341 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    11401 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    11461 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    11521 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    11581 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    11641 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    11701 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    11761 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    11821 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    11881 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    11941 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    12001 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    12061 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    12121 aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
