LOCUS       sequence-298536-        1353 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  expression of biosynthetic enzymes 4CL and STS in fusion with CC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Fink T, Stevovic B, Verwaal R, Roubos JA, Gaber R, Bencina M, Jerala
            R, Gradisar H
  TITLE     Metabolic enzyme clustering by coiled coils improves the 
            biosynthesis of resveratrol and mevalonate.
  JOURNAL   AMB Express. 2020 May 24;10(1):97. doi: 10.1186/s13568-020-01031-5.
  PUBMED    32448937
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "AMB 
            Express."; date: "2020-05-24"; volume: "10(1):97. doi"; pages: " 
            10.1186/s13568-020-01031-5"
COMMENT     Sequence Label: pET19b_4CL:P5SHb + STS:P6SHb
FEATURES             Location/Qualifiers
     source          1..1353
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1288..1302)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(1318..1347)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ttaacgtttc aaatactcaa tcttgcgctc taattcgcgg ttttcctcct ccaaacgctc
       61 gatttcgcgc tttaactcct cattcttgtc ctctggggag ccgctgccat tggtaaccat
      121 cggaatgcta tgcagaacaa cggtttcaat ggtcagaccc ggaccaaaac caaacagaac
      181 accccaatcc agaccttcac cggtggttgc acgttcacct ttcaggcttt ttttgcgcat
      241 ttcatccaga ataaacagaa cacatgcgct gctcatatta ccatattcgc tcagaacatg
      301 acgggttgct tccagtttct ttttgtccag gttcagtttt gcttcaactg catccagaat
      361 tgccggacca cccggatgtg caatccaaaa caggctattc caatcgctaa tacccagcgg
      421 atcaaatgcc tgggtcagac atttttcaac attttcgcta atcagggtcg gaacattcgg
      481 ccacagatga aaggtcagac caacttcacg cagattacct gcaattgcac cggcactatt
      541 cggaataaag gtctgtgctg cgctaaccag ctgaaacagc ggacgttcaa tgctaatatc
      601 cggatcgcta ccaacaataa ctgctgcgct accatcacca aacagtgcct gaccaaccag
      661 gctatccagt gcatcttcgc tcggaccacg aaaggtaaca acggtaattt cgctacaaac
      721 aaccagaaca cgtgcaccgg cattattttc tgccagatct tttgcggtac gcagaacggt
      781 gccaccggca taacaaccct gatgatacag cataacacga cgaacgctcg gttccagacc
      841 cagcagattt gccagtttat aatctgcacc aggcatttca acaccactgg tggtacaaaa
      901 aaccagatgg gtaattttgc ttttcggctg accccattct ttcagggctt tcagtgctgc
      961 ttctttaccc agtttcggaa cttctgcggt gataatttct tgacgaatat tcaggctcgg
     1021 tgccatatat gcaccaatat tcggatgttc ttccagcatt tcttcggtca ggtggatata
     1081 gcgttttttg atcatgcttt tatcgcagat gcggttaaat ttctttttca gtgcggtcat
     1141 gtgttcgctt ttggtcactt taaagtaata atcggcataa tcgctctgat aaacacaatg
     1201 atccggtgtt gcggtgccaa ttgccagaat ggttgccgga ccttttgcac gctgtgcatt
     1261 acgaaattct tcaacgcttg ccatatgctt gtcgtcgtcg tcgatatggc cgctgctgtg
     1321 atgatgatga tgatgatgat gatgatggcc cat
//
