LOCUS       sequence-298681-       10472 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses untagged human MCIDAS in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10472)
  TITLE     lentiviral expression plasmids for ciliary genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-EF1-alpha-IRES-EGFP-hMCIDAS
FEATURES             Location/Qualifiers
     source          1..10472
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(67..641,642..644,645..653)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(234..251)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     461..480
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(654..656,657..659,660..1373)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(654..656,657..659,660..1370)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(699..720)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(960..979)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1307..1328
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1383..1971
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1436..1456)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1854..1865)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2495..2675
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2701..2718)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2707..2931
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2977..3405
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3064..3083)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3274..3295
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3414..3434)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3419..3748
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3599..3734
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3661..3680
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3796..3843
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3862..4236
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4366..4487
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4403..4422)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4457..4476
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4536..4552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4536..4552)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4549..4571)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4560..4576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4584..4590,4591..4608,4609..4614))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4629..4650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4767..4784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4938..5526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5018..5037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5697..6488,6489..6557))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6320..6339
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6558..6662)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6737..6756)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6928..7307
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7309..7507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7462..7482
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7525..7705
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7752..7877
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8370..8603
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8788..8832
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9130..9247
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9294..10472
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9525..10463
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10420..10440
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggaattcggc cattacggcc gctagcgtta acgtcgacgg ccgcctcggc catcgataag
       61 gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
      121 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
      181 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
      241 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
      301 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
      361 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
      421 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
      481 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
      541 tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
      601 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtga
      661 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      721 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      781 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      841 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
      901 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      961 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     1021 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     1081 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     1141 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1201 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1261 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     1321 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc
     1381 gcaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     1441 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     1501 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     1561 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     1621 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     1681 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     1741 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     1801 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     1861 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     1921 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgcctgca
     1981 ggtttgtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     2041 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     2101 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     2161 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     2221 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     2281 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     2341 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     2401 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     2461 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     2521 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     2581 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     2641 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     2701 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     2761 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     2821 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     2881 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     2941 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     3001 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3061 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3121 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3181 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     3241 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     3301 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     3361 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     3421 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3481 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3541 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3601 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3661 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     3721 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     3781 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     3841 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     3901 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     3961 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     4021 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     4081 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     4141 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     4201 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     4261 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     4321 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     4381 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4441 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     4501 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     4561 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     4621 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     4681 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     4741 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4801 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4861 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4921 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4981 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5041 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5101 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5161 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5221 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5281 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5341 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5401 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5461 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5521 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5581 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     5641 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5701 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5761 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5821 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5881 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5941 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6001 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6061 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6121 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6181 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6241 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6301 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6361 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6421 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     6481 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     6541 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6601 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6661 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
     6721 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     6781 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     6841 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     6901 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
     6961 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     7021 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     7081 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     7141 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     7201 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     7261 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     7321 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     7381 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     7441 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     7501 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     7561 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7621 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7681 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     7741 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     7801 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     7861 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     7921 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     7981 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     8041 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     8101 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     8161 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     8221 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     8281 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     8341 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     8401 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     8461 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     8521 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     8581 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     8641 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     8701 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     8761 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     8821 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     8881 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     8941 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     9001 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     9061 attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt
     9121 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     9181 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     9241 aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta attggctccg
     9301 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg tggggagggg
     9361 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     9421 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     9481 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     9541 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     9601 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     9661 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     9721 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     9781 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     9841 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     9901 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     9961 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
    10021 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
    10081 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
    10141 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
    10201 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
    10261 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
    10321 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
    10381 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
    10441 aaagtttttt tcttccattt caggtgtcgt ga
//
