LOCUS       sequence-29883-e         795 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Jeanneteau F, Garabedian MJ, Chao MV
  TITLE     Activation of Trk neurotrophin receptors by glucocorticoids provides
            a neuroprotective effect.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Mar 25. 105(12):4862-7.
  PUBMED    18347336
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Mar 25. 105(12):4862-7."
COMMENT     Sequence Label: shRNA GR
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        102..414
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     333..353
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     479..495
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     480..496
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(538..571)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 caactaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
       61 cagcagagat ccagtttggt tagtaccggg cccgctctag agatccgacg cgccatctct
      121 aggcccgcgc cggccccctc gcacagactt gtgggagaag ctcggctact cccctgcccc
      181 ggttaatttg catataatat ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca
      241 gataatctgt tctttttaat actagctaca ttttacatga taggcttgga tttctataag
      301 agatacaaat actaaattat tattttaaaa aacagcacaa aaggaaactc accctaactg
      361 taaagtaatt gtgtgttttg agactataaa tatcccttgg agaaaagcct tgtttgcggg
      421 agaagatgat ccattcttca agagagaatg gatcatcttc tcccgctttt ttgaattctc
      481 gaggtcgacg gtatcgataa gctcgcttca cgagattcca gcaggtcgag ggacctaata
      541 acttcgtata gcatacatta tacgaagtta tattaagggt tccaagctta agcggccgcg
      601 tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta
      661 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      721 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      781 ccaataggga ctttc
//
