LOCUS       sequence-299717-        4979 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Luciferase reporter plasmid for FOXTF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Moparthi L, Koch S
  TITLE     A uniform expression library for the exploration of FOX 
            transcription factor biology.
  JOURNAL   Differentiation. 2020 Sep - Oct;115:30-36. doi: 
            10.1016/j.diff.2020.08.002. Epub 2020 Aug 20.
  PUBMED    32858261
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Differentiation. 2020 Sep - Oct"; volume: "115"; pages: "30-36. 
            doi: 10.1016/j.diff.2020.08.002. Epub 2020 Aug 20"
COMMENT     Sequence Label: 10 x FHR-luc
FEATURES             Location/Qualifiers
     source          1..4979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        156..187
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      243..252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             249..1901
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(315..333)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    1942..2063
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1953..1972)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2007..2026
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2311..2328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2482..3070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2562..2581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3241..4032,4033..4101))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3864..3883
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4102..4206)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4233..4688
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4320..4339)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4530..4551
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    4819..4867
                     /note="synthetic polyadenylation signal"
     misc_feature    4881..4972
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4921..4940
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tccgtaagta aacacgtaag taaacacgta agtaaacacg taagtaaaca
       61 cgtaagtaaa cacgtaagta aacacgtaag taaacacgta agatttaaac gtaagtaaac
      121 acgtaagatt taaacgtaac tcgagatcta gactctagag ggtatataat ggaagctcga
      181 attccagctt ggcattccgg tactgttggt aaaaagcttg gcattccggt actgttggta
      241 aagccaccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg
      301 aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
      361 gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg
      421 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
      481 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
      541 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
      601 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga
      661 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt
      721 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
      781 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact
      841 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga
      901 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
      961 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
     1021 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc
     1081 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa
     1141 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc
     1201 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc
     1261 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     1321 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     1381 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta
     1441 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
     1501 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc
     1561 tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct
     1621 tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg
     1681 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     1741 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     1801 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     1861 tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta attctagagt cggggcggcc
     1921 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1981 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     2041 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     2101 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
     2161 ataaggatcc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     2221 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     2281 ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2341 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2401 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2461 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2521 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2581 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2641 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2701 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2761 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2821 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2881 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2941 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3001 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3061 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3121 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3181 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3241 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3301 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3361 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3421 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3481 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3541 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3601 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3661 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3721 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3781 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3841 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3901 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3961 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4021 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4081 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4141 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4201 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
     4261 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4321 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4381 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4441 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4501 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4561 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4621 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     4681 tacaatttgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     4741 ctcttcgcta ttacgccagc ccaagctacc atgataagta agtaatatta aggtacggga
     4801 ggtacttgga gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     4861 ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa
     4921 ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctatcgata
//
