LOCUS       sequence-299721-        8985 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for expression of C-terminally VA-tagged human 
            TARDBP-WT in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Pourhaghighi R, Ash PEA, Phanse S, Goebels F, Hu LZM, Chen S, Zhang 
            Y, Wierbowski SD, Boudeau S, Moutaoufik MT, Malty RH, Malolepsza E, 
            Tsafou K, Nathan A, Cromar G, Guo H, Abdullatif AA, Apicco DJ, 
            Becker LA, Gitler AD, Pulst SM, Youssef A, Hekman R, Havugimana PC, 
            White CA, Blum BC, Ratti A, Bryant CD, Parkinson J, Lage K, Babu M, 
            Yu H, Bader GD, Wolozin B, Emili A
  TITLE     BraInMap Elucidates the Macromolecular Connectivity Landscape of 
            Mammalian Brain.
  JOURNAL   Cell Syst. 2020 Apr 22;10(4):333-350.e14. doi: 
            10.1016/j.cels.2020.03.003.
  PUBMED    32325033
REFERENCE   2  (bases 1 to 8985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2020.03"; journalName: "Cell Syst"; date: 
            "2020-04-22- 22"; volume: "10"; issue: "4"; pages: "333-350.e14"
COMMENT     Sequence Label: pLD-puro-Cc-TARDBP-WT_VA
FEATURES             Location/Qualifiers
     source          1..8985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     464..484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     510..534
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    565..589
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1841..1865)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1945..1968
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2005..2028
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2041..2058
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2068..2088
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2101..2121
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2134..2199
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2247..2364
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2413..2923
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2842..2860
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2945..3544
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2945..2964)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3441..3461
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3672..3905
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3977..4098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4014..4033)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4068..4087
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4138..4273
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4293..4312)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4294..4312)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4322..4339)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4322..4338)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4331..4353)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4480..4935
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4567..4586)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4777..4798
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4961..5065
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5066..5134,5135..5926)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5284..5303)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6097..6685
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6586..6605
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6839..6856
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6973..6994
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7009..7014,7015..7032,7033..7039)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7047..7063
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7052..7074
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7071..7087
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7071..7087
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7106..7126
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7108..7126
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7154..7380
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7381..7561
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7608..7733
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8226..8459
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8644..8688
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      541 agacgccatc cacgctgtac gcgtacaagt ttgtacaaaa aagcaggctc caccatgtct
      601 gaatatattc gggtaaccga agatgagaac gatgagccca ttgaaatacc atcggaagac
      661 gatgggacgg tgctgctctc cacggttaca gcccagtttc caggggcgtg tgggcttcgc
      721 tacaggaatc cagtgtctca gtgtatgaga ggtgtccggc tggtagaagg aattctgcat
      781 gccccagatg ctggctgggg aaatctggtg tatgttgtca actatccaaa agataacaaa
      841 agaaaaatgg atgagacaga tgcttcatca gcagtgaaag tgaaaagagc agtccagaaa
      901 acatccgatt taatagtgtt gggtctccca tggaaaacaa ccgaacagga cctgaaagag
      961 tattttagta cctttggaga agttcttatg gtgcaggtca agaaagatct taagactggt
     1021 cattcaaagg ggtttggctt tgttcgtttt acggaatatg aaacacaagt gaaagtaatg
     1081 tcacagcgac atatgataga tggacgatgg tgtgactgca aacttcctaa ttctaagcaa
     1141 agccaagatg agcctttgag aagcagaaaa gtgtttgtgg ggcgctgtac agaggacatg
     1201 actgaggatg agctgcggga gttcttctct cagtacgggg atgtgatgga tgtcttcatc
     1261 cccaagccat tcagggcctt tgcctttgtt acatttgcag atgatcagat tgcgcagtct
     1321 ctttgtggag aggacttgat cattaaagga atcagcgttc atatatccaa tgccgaacct
     1381 aagcacaata gcaatagaca gttagaaaga agtggaagat ttggtggtaa tccaggtggc
     1441 tttgggaatc agggtggatt tggtaatagc agagggggtg gagctggttt gggaaacaat
     1501 caaggtagta atatgggtgg tgggatgaac tttggtgcgt tcagcattaa tccagccatg
     1561 atggctgccg cccaggcagc actacagagc agttggggta tgatgggcat gttagccagc
     1621 cagcagaacc agtcaggccc atcgggtaat aaccaaaacc aaggcaacat gcagagggag
     1681 ccaaaccagg ccttcggttc tggaaataac tcttatagtg gctctaattc tggtgcagca
     1741 attggttggg gatcagcatc caatgcaggg tcgggcagtg gttttaatgg aggctttggc
     1801 tcaagcatgg attctaagtc ttctggctgg ggaatgttgg acccagcttt cttgtacaaa
     1861 gtggttgatt ctagagcgat cgccagagag ctgttcaacc tgctgggcga gaaccagccc
     1921 cccgtggtga tcaaggcgat cgcctggagc caccctcagt tcgagaaggg aggaggaagc
     1981 ggcggaggca gcggaggagg aagctggagc cacccgcagt tcgagaaagg cggaagcggc
     2041 catcaccatc accatcaccc tgcaggagaa aatctgtact tccaggggag aggaggcagc
     2101 gagaacctgt actttcaggg gcctgcagga agagactaca aagaccatga cggtgattat
     2161 aaagatcatg acatcgatta caaggatgac gatgacaagt agttcgaatt ctcgacctcg
     2221 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     2281 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     2341 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
     2401 ccgcggctcg agggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg
     2461 acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac
     2521 attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc
     2581 ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg
     2641 gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg
     2701 agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg
     2761 cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg
     2821 ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc
     2881 agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc caccggagct
     2941 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     3001 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     3061 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     3121 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     3181 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     3241 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     3301 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     3361 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     3421 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     3481 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     3541 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
     3601 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     3661 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     3721 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     3781 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     3841 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     3901 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     3961 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4021 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4081 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     4141 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4201 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4261 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     4321 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     4381 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     4441 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     4501 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4561 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4621 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4681 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4741 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4801 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4861 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     4921 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4981 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5041 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5101 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5161 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5221 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5281 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5341 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5401 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5461 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5521 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5581 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5641 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5701 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5761 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5821 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5881 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5941 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6001 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6061 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6121 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6181 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6241 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6301 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6361 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6421 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6481 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6541 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6601 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6661 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6721 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6781 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6841 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     6901 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     6961 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     7021 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     7081 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     7141 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     7201 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     7261 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     7321 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     7381 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7441 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7501 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7561 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     7621 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     7681 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     7741 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     7801 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     7861 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     7921 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     7981 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     8041 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     8101 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     8161 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     8221 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     8281 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     8341 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     8401 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     8461 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     8521 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     8581 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     8641 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     8701 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     8761 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     8821 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     8881 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     8941 cggtatcgat cacgagacta gcctcgagcg gccgccccct tcacc
//
