LOCUS       sequence-299921-         468 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease 
            cleavable Strep tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 468)
  TITLE     Plasmids for the Expression and Co-Expression of COVID-19 Proteins 
            Nsp10 and Nsp14
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET22-Strep-TEV-Nsp10
FEATURES             Location/Qualifiers
     source          1..468
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             28..48
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtggtcac acccccaatt tgaaaaggaa aatctatact tccaaggagc tggtaatgca
       61 actgaggttc ccgcgaatag tacggttctg agcttttgcg cctttgctgt tgacgcagct
      121 aaggcatata aggactactt ggcctccggg ggccagccaa tcacaaactg cgtcaagatg
      181 ttgtgtacgc acacgggcac aggtcaggca atcactgtca cacctgaagc taacatggat
      241 caggaatctt ttggtggtgc cagttgttgt ctgtactgtc gatgtcatat agatcatccc
      301 aacccgaaag ggttttgtga tctgaaagga aaatacgtcc aaatcccgac cacgtgcgcg
      361 aacgacccgg ttgggtttac gttgaaaaat accgtatgta ctgtctgtgg tatgtggaag
      421 ggctatggct gttcatgcga tcaattgcga gagccgatgt tgcagtaa
//
