LOCUS       sequence-299987-        9328 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Express His-MBP-TEV-CasRx recombinant protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Brogan DJ, Chaverra-Rodriguez D, Lin CP, Smidler AL, Yang T, 
            Alcantara LM, Antoshechkin I, Liu J, Raban RR, Belda-Ferre P, Knight
            R, Komives EA, Akbari OS
  TITLE     Development of a Rapid and Sensitive CasRx-Based Diagnostic Assay 
            for SARS-CoV-2.
  JOURNAL   ACS Sens. 2021 Nov 26;6(11):3957-3966. doi: 
            10.1021/acssensors.1c01088. Epub 2021 Oct 29.
  PUBMED    34714054
REFERENCE   2  (bases 1 to 9328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.1c01088"; journalName: "ACS Sens"; date: 
            "2021-11-26- 26"; volume: "6"; issue: "11"; pages: "3957-3966"
COMMENT     Sequence Label: OA-1136J
FEATURES             Location/Qualifiers
     source          1..9328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     544..563
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(583..660)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     866..885
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     969..988
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        969..987
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    988..1012
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1027..1049
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1068..1085
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1095..2195
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2169..2192
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2208..2228
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2232..5129
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVXLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             5160..5177
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5230..5248)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5244..5291
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5328..5783
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5415..5434)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5625..5646
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5876..6691)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5876..6691)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6599..6618
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6683..6702
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6813..7401
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7302..7321
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7555..7572
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7587..7726
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7652..7671)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7812..7834
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7828..8019)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(8828..9328,1..582))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
       61 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
      121 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
      181 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
      241 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
      301 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
      361 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
      421 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
      481 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac
      541 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg
      601 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc
      661 cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
      721 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
      781 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
      841 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
      901 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc
      961 cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt cccctctaga
     1021 aataattttg tttaacttta agaaggagat ataccatggg ttcttctcac catcaccatc
     1081 accatggttc ttctatgaaa atcgaagaag gtaaactggt aatctggatt aacggcgata
     1141 aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag
     1201 tcaccgttga gcatccggat aaactggaag agaaattccc acaggttgcg gcaactggcg
     1261 atggccctga cattatcttc tgggcacacg accgctttgg tggctacgct caatctggcc
     1321 tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacctggg
     1381 atgccgtacg ttacaacggc aagctgattg cttacccgat cgctgttgaa gcgttatcgc
     1441 tgatttataa caaagatctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc
     1501 tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt
     1561 acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca
     1621 agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc
     1681 tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag
     1741 ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca
     1801 tcgacaccag caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat
     1861 ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc cgccagtccg aacaaagagc
     1921 tggcaaaaga gttcctcgaa aactatctgc tgactgatga aggtctggaa gcggttaata
     1981 aagacaaacc gctgggtgcc gtagcgctga agtcttacga ggaagagttg gcgaaagatc
     2041 cacgtattgc cgccactatg gaaaacgccc agaaaggtga aatcatgccg aacatcccgc
     2101 agatgtccgc tttctggtat gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc
     2161 agactgtcga tgaagccctg aaagacgcgc agactaatgg gatcgaggaa aacctgtact
     2221 tccaatccaa tatcgaaaaa aaaaagtcct tcgccaaggg catgggcgtg aagtccacac
     2281 tcgtgtccgg ctccaaagtg tacatgacaa ccttcgccga aggcagcgac gccaggctgg
     2341 aaaagatcgt ggagggcgac agcatcagga gcgtgaatga gggcgaggcc ttcagcgctg
     2401 aaatggccga taaaaacgcc ggctataaga tcggcaacgc caaattcagc catcctaagg
     2461 gctacgccgt ggtggctaac aaccctctgt atacaggacc cgtccagcag gatatgctcg
     2521 gcctgaagga aactctggaa aagaggtact tcggcgagag cgctgatggc aatgacaata
     2581 tttgtatcca ggtgatccat aacatcctgg acattgaaaa aatcctcgcc gaatacatta
     2641 ccaacgccgc ctacgccgtc aacaatatct ccggcctgga taaggacatt attggattcg
     2701 gcaagttctc cacagtgtat acctacgacg aattcaaaga ccccgagcac catagggccg
     2761 ctttcaacaa taacgataag ctcatcaacg ccatcaaggc ccagtatgac gagttcgaca
     2821 acttcctcga taaccccaga ctcggctatt tcggccaggc ctttttcagc aaggagggca
     2881 gaaattacat catcaattac ggcaacgaat gctatgacat tctggccctc ctgagcggac
     2941 tgaggcactg ggtggtccat aacaacgaag aagagtccag gatctccagg acctggctct
     3001 acaacctcga taagaacctc gacaacgaat acatctccac cctcaactac ctctacgaca
     3061 ggatcaccaa tgagctgacc aactccttct ccaagaactc cgccgccaac gtgaactata
     3121 ttgccgaaac tctgggaatc aaccctgccg aattcgccga acaatatttc agattcagca
     3181 ttatgaaaga gcagaaaaac ctcggattca atatcaccaa gctcagggaa gtgatgctgg
     3241 acaggaagga tatgtccgag atcaggaaaa atcataaggt gttcgactcc atcaggacca
     3301 aggtctacac catgatggac tttgtgattt ataggtatta catcgaagag gatgccaagg
     3361 tggctgccgc caataagtcc ctccccgata atgagaagtc cctgagcgag aaggatatct
     3421 ttgtgattaa cctgaggggc tccttcaacg acgaccagaa ggatgccctc tactacgatg
     3481 aagctaatag aatttggaga aagctcgaaa atatcatgca caacatcaag gaatttaggg
     3541 gaaacaagac aagagagtat aagaagaagg acgcccctag actgcccaga atcctgcccg
     3601 ctggccgtga tgtttccgcc ttcagcaaac tcatgtatgc cctgaccatg ttcctggatg
     3661 gcaaggagat caacgacctc ctgaccaccc tgattaataa attcgataac atccagagct
     3721 tcctgaaggt gatgcctctc atcggagtca acgctaagtt cgtggaggaa tacgcctttt
     3781 tcaaagactc cgccaagatc gccgatgagc tgaggctgat caagtccttc gctagaatgg
     3841 gagaacctat tgccgatgcc aggagggcca tgtatatcga cgccatccgt attttaggaa
     3901 ccaacctgtc ctatgatgag ctcaaggccc tcgccgacac cttttccctg gacgagaacg
     3961 gaaacaagct caagaaaggc aagcacggca tgagaaattt cattattaat aacgtgatca
     4021 gcaataaaag gttccactac ctgatcagat acggtgatcc tgcccacctc catgagatcg
     4081 ccaaaaacga ggccgtggtg aagttcgtgc tcggcaggat cgctgacatc cagaaaaaac
     4141 agggccagaa cggcaagaac cagatcgaca ggtactacga aacttgtatc ggaaaggata
     4201 agggcaagag cgtgagcgaa aaggtggacg ctctcacaaa gatcatcacc ggaatgaact
     4261 acgaccaatt cgacaagaaa aggagcgtca ttgaggacac cggcagggaa aacgccgaga
     4321 gggagaagtt taaaaagatc atcagcctgt acctcaccgt gatctaccac atcctcaaga
     4381 atattgtcaa tatcaacgcc aggtacgtca tcggattcca ttgcgtcgag cgtgatgctc
     4441 aactgtacaa ggagaaaggc tacgacatca atctcaagaa actggaagag aagggattca
     4501 gctccgtcac caagctctgc gctggcattg atgaaactgc ccccgataag agaaaggacg
     4561 tggaaaagga gatggctgaa agagccaagg agagcattga cagcctcgag agcgccaacc
     4621 ccaagctgta tgccaattac atcaaataca gcgacgagaa gaaagccgag gagttcacca
     4681 ggcagattaa cagggagaag gccaaaaccg ccctgaacgc ctacctgagg aacaccaagt
     4741 ggaatgtgat catcagggag gacctcctga gaattgacaa caagacatgt accctgttca
     4801 gaaacaaggc cgtcyacctg gaagtggcca ggtatgtcca cgcctatatc aacgacattg
     4861 ccgaggtcaa ttcctacttc caactgtacc attacatcat gcagagaatt atcatgaatg
     4921 agaggtacga gaaaagcagc ggaaaggtgt ccgagtactt cgacgctgtg aatgacgaga
     4981 agaagtacaa cgataggctc ctgaaactgc tgtgtgtgcc tttcggctac tgtatcccca
     5041 ggtttaagaa cctgagcatc gaggccctgt tcgataggaa cgaggccgcc aagttcgaca
     5101 aggagaaaaa gaaggtgtcc ggcaattcct aagtcgacaa gcttgcggcc gcactcgagc
     5161 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     5221 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     5281 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
     5341 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5401 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5461 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5521 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5581 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5641 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     5701 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     5761 caaactagta acgtttacaa tttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5821 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga
     5881 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     5941 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     6001 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     6061 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     6121 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
     6181 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     6241 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     6301 accggcgcag gaacactgcc agcgcatcaa caatgttttc acctgaatca ggatattctt
     6361 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
     6421 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     6481 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     6541 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     6601 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag
     6661 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     6721 cagacagttt tattgttcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6781 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6841 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6901 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     6961 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7021 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7081 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7141 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7201 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7261 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7321 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7381 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7441 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7501 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7561 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     7621 ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     7681 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     7741 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     7801 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     7861 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg
     7921 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
     7981 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
     8041 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
     8101 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
     8161 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
     8221 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
     8281 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
     8341 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
     8401 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
     8461 caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc
     8521 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
     8581 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
     8641 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
     8701 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     8761 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     8821 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8881 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     8941 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     9001 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     9061 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
     9121 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     9181 ccagcatcgc agtgggaacg ataccctcat tcagcatttg catggtttgt tgaaaaccgg
     9241 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     9301 atttatgcca gccagccaga cgcagacg
//
