LOCUS       sequence-300177-        9657 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses high-affinity split-TurboID C-terminal fragment in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9657)
  AUTHORS   Cho KF, Branon TC, Rajeev S, Svinkina T, Udeshi ND, Thoudam T, Kwak 
            C, Rhee HW, Lee IK, Carr SA, Ting AY
  TITLE     Split-TurboID enables contact-dependent proximity labeling in cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 May 18. pii: 1919528117. doi: 
            10.1073/pnas.1919528117.
  PUBMED    32424107
REFERENCE   2  (bases 1 to 9657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 May 18. pii: 1919528117. doi: 
            10.1073/pnas.1919528117."
COMMENT     Sequence Label: pLX304 CMV HA-HaloTag-FRB-sTurboID-high affini...
FEATURES             Location/Qualifiers
     source          1..9657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    595..619
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             640..666
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    2664..3252
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2717..2737)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3135..3146)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3324..3557
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3635..3756
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3672..3691)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3726..3745
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3796..3931
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3951..3970)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3952..3970)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3980..3997)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3980..3996)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3989..4011)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4138..4593
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4225..4244)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4435..4456
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4619..4723
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4724..4792,4793..5584)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5755..6343
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6244..6263
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6497..6514
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6631..6652
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6667..6672,6673..6690,6691..6697)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6705..6721
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6710..6732
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6729..6745
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6729..6745
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6764..6784
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6766..6784
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6812..7038
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7039..7219
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7266..7391
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7884..8117
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8302..8346
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8521..9021
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8940..8958
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9033..9428
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9488..9605
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtacaaaa aagcaggctt cgaaggagat agaaccatgt acccatacga cgttccagac
      661 tacgcaatcg gtacaggctt ccccttcgac ccccattatg tggaagtcct gggcgagcgt
      721 atgcactacg tcgatgttgg accgcgggat ggcacgcctg tgctgttcct gcacggtaac
      781 ccgacctcgt cctacctgtg gcgcaacatc atcccgcatg tagcaccgag tcatcggtgc
      841 attgctccag acctgatcgg gatgggaaaa tcggacaaac cagacctcga ttatttcttc
      901 gacgaccacg tccgctacct cgatgccttc atcgaagcct tgggtttgga agaggtcgtc
      961 ctggtcatcc acgactgggg ctcagctctc ggattccact gggccaagcg caatccggaa
     1021 cgggtcaaag gtattgcatg tatggaattc atccggccta tcccgacgtg ggacgaatgg
     1081 ccagaattcg cccgtgagac cttccaggcc ttccggaccg ccgacgtcgg ccgagagttg
     1141 atcatcgatc agaacgcttt catcgagggt gcgctcccga tgggggtcgt ccgtccgctt
     1201 acggaggtcg agatggacca ctatcgcgag cccttcctca agcctgttga ccgagagcca
     1261 ctgtggcgat tccccaacga gctgcccatc gccggtgagc ccgcgaacat cgtcgcgctc
     1321 gtcgaggcat acatgaactg gctgcaccag tcacctgtcc cgaagttgtt gttctggggc
     1381 acacccggcg tactgatccc cccggccgaa gccgcgagac ttgccgaaag cctccccaac
     1441 tgcaagacag tggacatcgg cccgggattg ttcttgctcc aggaagacaa cccggacctt
     1501 atcggcagtg agatcgcgcg ctggctctcg acgctcgaaa tttccggcgc tagcaccaaa
     1561 ggttctggtg ggactggcgg gagcgtggcc atcctgtggc acgagatgtg gcatgaaggc
     1621 ctggaagagg catctcgttt gtactttggg gaaaggaacg tgaaaggcat gtttgaggtg
     1681 ctggagccct tgcatgctat gatggaacgg ggcccccaga ctctgaagga aacatccttt
     1741 aatcaggcct atggtcgaga tttaatggag gcccaagagt ggtgcaggaa gtacatgaaa
     1801 tcagggaatg tcaaggacct cacccaagcc tgggacctct attatcatgt gttccgacga
     1861 atctcaggat ccaagggctc gggctcgacc tcgggctcgg gcgggagcgt ggcagtcctg
     1921 cctgtggtcg actccaccaa tcagtacctg ctggatcgaa tcggcgagct gaagagtggg
     1981 gatgcttgca ttgcagaata tcagcaggca gggagaggaa gcagagggag gaaatggttc
     2041 tctccttttg gagctaacct gtacctgagt atgttttggc gcctgaagcg gggaccagca
     2101 gcaatcggcc tgggcccggt catcggaatt gtcatggcag aagcgctgcg aaagctggga
     2161 gcagacaagg tgcgagtcaa atggcccaat gacctgtatc tgcaggatag aaagctggca
     2221 ggcatcctgg tggagctggc cggaataaca ggcgatgctg cacagatcgt cattggcgcc
     2281 gggattaacg tggctatgag gcgcgtggag gaaagcgtgg tcaatcaggg ctggatcaca
     2341 ctgcaggaag cagggattaa cctggacagg aatactctgg ccgctacgct gatccgagag
     2401 ctgcgggcag ccctggaact gttcgagcag gaaggcctgg ctccatatct gccacggtgg
     2461 gagaagctgg ataacttcat caatagaccc gtgaagctga tcattgggga caaagagatt
     2521 ttcgggatta gccgggggat tgataaacag ggagccctgc tgctggaaca ggacggagtt
     2581 atcaaaccct ggatgggcgg agaaatcagt ctgcggtctg ccgaaaagta agctagcgct
     2641 aaccggtggc gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     2701 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     2761 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     2821 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     2881 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     2941 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     3001 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     3061 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     3121 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     3181 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     3241 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     3301 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     3361 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     3421 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     3481 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     3541 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     3601 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     3661 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3721 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     3781 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3841 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3901 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     3961 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     4021 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     4081 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     4141 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     4201 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     4261 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     4321 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     4381 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     4441 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     4501 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4561 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     4621 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4681 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4741 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4801 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4861 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4921 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4981 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5041 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5101 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5161 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5221 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5281 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5341 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5401 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5461 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5521 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5581 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     5641 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5701 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5761 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5821 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5881 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5941 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6001 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6061 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6121 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6181 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6241 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6301 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6361 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6421 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6481 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     6541 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     6601 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     6661 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     6721 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     6781 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     6841 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     6901 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     6961 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     7021 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7081 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7141 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7201 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     7261 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     7321 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     7381 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     7441 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     7501 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     7561 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     7621 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     7681 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     7741 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     7801 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     7861 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     7921 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     7981 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     8041 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     8101 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     8161 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     8221 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     8281 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     8341 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     8401 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     8461 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     8521 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     8581 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     8641 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     8701 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     8761 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     8821 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     8881 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     8941 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     9001 atcaccgacc tctctcccca gggggtacca ccatggccaa gcctttgtct caagaagaat
     9061 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
     9121 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
     9181 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
     9241 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
     9301 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
     9361 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
     9421 gggagggcct gcagctgcag tagtaagaat tctagatctt gagacaaatg gcagtattca
     9481 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     9541 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     9601 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gaggggg
//
