LOCUS       sequence-300235-       10422 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 S (surface 
            glycoprotein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10422)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 10422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4134233
FEATURES             Location/Qualifiers
     source          1..10422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4402..4422
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             4423..4464
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    4533..4654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4544..4563)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4598..4617
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4769..4815
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4845..4981
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5026..5097
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(5323..5911)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5403..5422)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        6411..7620
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7457..7476
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             7624..8652
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    8679..8727
                     /note="synthetic polyadenylation signal"
     CDS             join(8993..9061,9062..9853)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9211..9230)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      9866..10002
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    10154..10225
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(10230..10276)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    10294..10342
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catgctgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatca gatctttgtc
      541 gatcctacca tccactcgac acacccgcca gcggccgcca ccatgttcgt ctttcttgtg
      601 ttactccccc tggtttcgtc ccagtgcgtg aacttgacta cgcgaaccca actgccgcca
      661 gcatatacaa atagtttcac ccgtggagta tactaccctg ataaagtgtt tagatcaagc
      721 gtactacact ctactcagga cctgtttctc cccttcttct ccaatgtcac atggtttcat
      781 gcgatccacg ttagcggcac caacgggaca aagcgctttg acaaccctgt gctgccattc
      841 aatgatggtg tgtattttgc ttctaccgag aagagcaaca taattcgggg ctggatcttc
      901 ggaactaccc tcgacagtaa aactcagtca ttgctgattg tgaataacgc cacaaatgtc
      961 gttatcaagg tctgtgaatt tcaattctgc aacgatccat tccttggggt gtattaccat
     1021 aaaaataata agtcctggat ggagagcgaa tttagggtat actcgtctgc caacaattgt
     1081 actttcgagt atgtgtctca gcccttctta atggatctgg aaggtaaaca gggcaacttt
     1141 aagaacctcc gcgagtttgt attcaaaaat atagacggat actttaagat ttattccaag
     1201 cacacaccaa tcaatttggt gcgggatctc ccgcaagggt tcagcgctct ggaacctcta
     1261 gtggacctcc ccatcggcat taacatcaca agattccaga cgctgttagc cctgcataga
     1321 tcatacctta ctcccggtga cagtagttct gggtggaccg caggcgcggc tgcatattac
     1381 gtgggatatt tgcagcctcg gacgtttctg ctgaaataca acgagaacgg caccattaca
     1441 gatgccgtgg attgcgccct tgacccccta agcgaaacta agtgtacgct taaaagcttc
     1501 accgtcgaga agggaattta ccagacttcc aactttaggg tccagccgac agaatcaata
     1561 gttaggttcc caaatatcac caatttgtgc ccttttgggg aagtcttcaa tgctacacga
     1621 tttgcttcgg tttatgcctg gaacaggaag cgtatctcaa attgcgtcgc ggactacagc
     1681 gtgctctata atagtgcaag tttctcgacc tttaaatgtt atggcgtgtc cccgaccaaa
     1741 ctaaatgacc tctgttttac aaacgtttac gccgattctt ttgtgattcg cggtgatgag
     1801 gtacgccaaa tcgcaccggg ccagactggc aagattgccg attacaacta caagttgcct
     1861 gacgacttca ccggatgtgt tatagcctgg aatagcaata atctggattc taaggtcgga
     1921 ggtaactata actacctgta tagactgttc cgtaagtcaa acctgaaacc ctttgagcgg
     1981 gacatctcca cagagattta ccaagcaggg tctaccccct gcaatggggt tgaaggattc
     2041 aattgctact ttccattaca gagctacggg ttccagccta cgaacggggt tggttaccag
     2101 ccttataggg tagtggtgct gtcgttcgaa ctgttgcacg ctccagctac agtgtgcggg
     2161 cctaaaaaat caactaacct ggttaaaaat aagtgtgtta attttaactt caatggctta
     2221 accggaactg gagtgctcac ggagtcaaac aagaaatttc taccgtttca gcaattcggg
     2281 agggatatcg cagacactac ggacgcggtc agagatccac agacccttga gatccttgac
     2341 ataacaccct gctctttcgg aggtgtctcc gtaattacac caggcactaa tacctctaat
     2401 caggtggccg tgctttatca ggatgttaac tgtacagagg tcccggtggc tatccatgcc
     2461 gaccaattga cacctacgtg gcgcgtctat tctaccggct ccaacgtctt ccagacccgg
     2521 gctggatgct tgataggcgc agaacacgta aacaactctt acgaatgtga tattcccatt
     2581 ggagccggga tctgcgcaag ctaccagacc caaacaaaca gtcccagacg ggctcgtagc
     2641 gtggcctctc agagcataat agcctatacc atgtcccttg gcgcggagaa tagtgtggca
     2701 tactcaaata atagcatcgc tatccccact aactttacta tttccgtgac gactgagatt
     2761 ctgccagtgt ctatgactaa gacatccgtg gattgcacaa tgtatatttg tggggattcc
     2821 acagagtgta gcaacttgtt gctgcaatat ggatcattct gtacgcagct gaatcgcgcc
     2881 ctcaccggca tcgctgtgga acaggacaag aacactcaag aggtctttgc tcaggtgaag
     2941 caaatctaca agaccccacc tataaaagac ttcgggggtt ttaacttctc gcagattctc
     3001 cccgacccca gcaagccttc aaaaaggagc tttattgagg acctgttgtt caacaaggtt
     3061 actttagccg atgccggttt tatcaaacag tacggcgact gtcttggcga tatagcagcc
     3121 cgggatctga tttgcgcaca aaaattcaat ggccttaccg tactgccacc ccttctcacc
     3181 gacgaaatga tcgcgcagta cacctccgca ctgctggctg ggaccatcac ttcgggatgg
     3241 acctttgggg ctggtgctgc tttgcaaatt cccttcgcca tgcagatggc ctatcgattc
     3301 aatggcatag gggtaacaca gaacgtattg tatgaaaatc aaaagctaat cgctaaccag
     3361 tttaattcag ccattggtaa gatccaggat tcactctcca gtacagcctc tgccctggga
     3421 aagctccagg acgtggtcaa ccagaacgcg caggcattga atacactcgt caaacagctg
     3481 agtagcaatt tcggcgcaat cagtagtgtc ttaaacgata tactgagtag gcttgataaa
     3541 gtagaggccg aggtccagat tgacagacta atcacggggc gtcttcagag tttacagacc
     3601 tatgtgaccc aacagctgat ccgagcagcg gaaattcgag caagcgcgaa cctggccgct
     3661 actaaaatga gtgagtgcgt gctgggccaa agtaaacgag tcgacttttg cggcaaaggg
     3721 tatcatctca tgtctttccc tcagtccgcc cctcacggtg ttgttttcct ccacgtgaca
     3781 tacgttcccg cgcaggaaaa gaattttact acggcacccg ctatttgcca tgacggaaag
     3841 gctcattttc cacgggaggg agtgttcgtt tcaaacggga cacactggtt tgtaactcag
     3901 cggaactttt atgagcctca aatcatcacg acagacaata ccttcgtgag tggcaactgc
     3961 gatgtagtca taggcattgt gaataacact gtttatgatc cacttcagcc ggaactcgac
     4021 agcttcaaag aggaattgga taagtacttt aaaaatcata ccagtccaga cgtagatctc
     4081 ggcgatatat cgggcattaa cgccagtgtg gtcaatatac aaaaggagat tgacagactg
     4141 aatgaagtag ctaagaacct caacgaatct ctgattgacc tgcaagagct agggaagtac
     4201 gagcagtata tcaaatggcc atggtacata tggctgggtt ttatcgcggg tcttattgcc
     4261 atagtcatgg tgacgattat gttgtgctgt atgacctcct gctgttcttg tttgaaaggc
     4321 tgctgctcct gtggtagctg ttgcaaattc gatgaagatg actctgaacc cgtgttgaaa
     4381 ggcgttaagc tacactacac agaaaacctt tactttcagt cggggaaacc tatacccaat
     4441 ccacttctcg gcttagattc tacctaagga tccgcaggcc tctgctagct tgactgactg
     4501 agatacagcg taccttcagc tcacagacat gataagatac attgatgagt ttggacaaac
     4561 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     4621 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     4681 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     4741 tggtattggc ccatctctat cggtatcgta gcataacccc ttggggcctc taaacgggtc
     4801 ttgaggggtt ttttgtgccc ctcgggccgg attgctatct accggcattg gcgcagaaaa
     4861 aaatgcctga tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga
     4921 tacggcttcc ccaacttgcc cacttccata cgtgtcctcc ttaccagaaa tttatcctta
     4981 aggtcgtcag ctatcctgca ggcgatctct cgatttcgat caagacattc ctttaatggt
     5041 cttttctgga caccactagg ggtcagaagt agttcatcaa actttcttcc ctccctaatc
     5101 tcattggtta ccttgggcta tcgaaactta attaaccagt caagtcagct acttggcgag
     5161 atcgacttgt ctgggtttcg actacgctca gaattgcgtc agtcaagttc gatctggtcc
     5221 ttgctattgc acccgttctc cgattacgag tttcatttaa atcatgtgag caaaaggcca
     5281 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5341 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5401 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5461 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5521 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5581 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5641 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5701 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5761 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     5821 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     5881 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5941 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6001 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6061 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6121 ctgtctattt cgttcatcca tagttgcatt taaatttccg aactctccaa ggccctcgtc
     6181 ggaaaatctt caaacctttc gtccgatcca tcttgcaggc tacctctcga acgaactatc
     6241 gcaagtctct tggccggcct tgcgccttgg ctattgcttg gcagcgccta tcgccaggta
     6301 ttactccaat cccgaatatc cgagatcggg atcacccgag agaagttcaa cctacatcct
     6361 caatcccgat ctatccgaga tccgaggaat atcgaaatcg gggcgcgcct ggcctccgcg
     6421 ccgggttttg gcgcctcccg cgggcgcccc cctcgtcacg gcgagcgctg ccacgtcaga
     6481 cgaagggcgc aggagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc
     6541 tcataagact cggccttaga accccagtat cagcagaagg acattttagg acgggacttg
     6601 ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc
     6661 cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag
     6721 gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg
     6781 tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt
     6841 tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta
     6901 gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg
     6961 cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa
     7021 caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgagcccttc gctaatgcgg
     7081 gaaagctctt attcgggtga gatgggctgg gcaccatctg gggaccctga cgtgaagttt
     7141 gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc
     7201 gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc
     7261 gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt
     7321 ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg
     7381 gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc
     7441 tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg
     7501 ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt
     7561 cagtgttaga cttgtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac
     7621 aacatgggta aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     7681 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     7741 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     7801 aaagatcgtt atgtttatcg gcactttgca tccgccgcgc tcccgattcc ggaagtgctt
     7861 gacattgggg agttcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     7921 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggca
     7981 atggatgcca tcgctgccgc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     8041 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     8101 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     8161 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     8221 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     8281 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     8341 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     8401 tcgccccggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     8461 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     8521 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgccgc cgtctggacc
     8581 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     8641 gcaaaggaat aagctagtat gtaagcctag tcttagataa taaaatcgct atccatcgaa
     8701 gatggatgtg tgttggtttt ttgtgtgtgt aacgctaggc gcgcctggtg taccgagaac
     8761 gatcctctca gtgcgagtct cgacgatcca tatcgttgct tggcagtcag ccagtcggaa
     8821 tccagcttgg gacccaggaa gtccaatcgt cagatattgt actcaagcct ggtcacggca
     8881 gcgtaccgat ctgtttaaac ctagatattg atagtctgat cggtcaacgt ataatcgagt
     8941 cctagctttt gcaaacatct atcaagagac aggatcagca ggaggctttc gcatgagtat
     9001 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     9061 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cgcgagtggg
     9121 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     9181 ctttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     9241 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     9301 ttcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     9361 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga ttggaggacc
     9421 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     9481 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     9541 aatggcaaca accttgcgta aactattaac tggcgaacta cttactctag cttcccggca
     9601 acagttgata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     9661 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     9721 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9781 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9841 taagcattgg taaccgattc taggtgcatt ggcgcagaaa aaaatgcctg atgcgacgct
     9901 gcgcgtctta tactcccaca tatgccagat tcagcaacgg atacggcttc cccaacttgc
     9961 ccacttccat acgtgtcctc cttaccagaa atttatcctt aagatcccga atcgtttaaa
    10021 ctcgactctg gctctatcga atctccgtcg tttcgagctt acgcgaacag ccgtggcgct
    10081 catttgctcg tcgggcatcg aatctcgtca gctatcgtca gcttaccttt ttggcagcga
    10141 tcgcggctcc cgacatcttg gaccattagc tccacaggta tcttcttccc tctagtggtc
    10201 ataacagcag cttcagctac ctctcaattc aaaaaacccc tcaagacccg tttagaggcc
    10261 ccaaggggtt atgctatcaa tcgttgcgtt acacacacaa aaaaccaaca cacatccatc
    10321 ttcgatggat agcgatttta ttatctaact gctgatcgag tgtagccaga tctagtaatc
    10381 aattacgggg tcattagttc atagcccata tatggagttc cg
//
