LOCUS       sequence-304279-       10153 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-SEMA5B-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5803..5826
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7486..7530
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7531..7557
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7697..7714)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7703..7814
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7932..7952)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7937..8266
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8117..8252
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8179..8198
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8314..8361
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8380..8979
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8380..8399)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8876..8896
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9109..9230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9146..9165)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9200..9219
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9283..10074,10075..10143))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9906..9925
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10144..10153,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgcctt gtctcttgtt
     2761 agaggcctgc ttccttgcct gccaccaggc gctagaacag ccgagggacc tattatggtg
     2821 ctggctggac ctctggccgt gtctctgctt ctgccttctc tgaccctgct ggtgtctcac
     2881 ctgtctagca gccaggatgt gtccagcgag cctagctctg agcagcagct gtgtgccctg
     2941 agcaagcacc ctacagtggc cttcgaagat ctgcagccct gggtgtccaa cttcacatac
     3001 cctggggcca gagacttctc ccagctggct cttgatccca gcggcaacca gctgattgtg
     3061 ggcgccagaa actacctgtt cagactgagc ctggccaacg tgtcactgct gcaggctaca
     3121 gagtgggcca gcagcgagga taccagaaga agctgtcaga gcaagggcaa gaccgaggaa
     3181 gagtgccaga actacgtgcg ggtgctgatc gtggccggca gaaaggtttt catgtgcggc
     3241 accaacgctt tcagccccat gtgcaccagc agacaagtgg gcaacctgag ccggaccatc
     3301 gagaagatca acggcgtggc cagatgtccc tacgatccca gacacaatag caccgccgtg
     3361 atcagctctc agggcgaact gtatgccgcc accgtgatcg atttcagcgg cagagatccc
     3421 gccatctaca gaagcctcgg atctggccct ccactgagaa cagcccagta caacagcaag
     3481 tggctgaacg agcccaactt cgtggccgcc tatgacatcg gcctgttcgc ctacttcttc
     3541 ctgcgggaaa acgccgtgga acacgactgt ggcagaaccg tgtatagcag agtggccaga
     3601 gtgtgcaaga acgacgtcgg cggcagattc ctgctggaag atacctggac cacctttatg
     3661 aaggcccggc tgaactgcag cagacctggc gaggtgccct tctactacaa cgagctgcag
     3721 agcgccttcc atctgcctga gcaggatctg atctacggcg tgttcaccac caacgtgaac
     3781 tctatcgccg cctctgccgt gtgcgccttt aatctgtctg ccatcagcca ggcctttaac
     3841 ggccccttca gataccaaga gaaccccaga gctgcctggc tgcctatcgc caatcctatt
     3901 cctaacttcc agtgcggcac actgcccgag acaggcccca atgagaatct gaccgagaga
     3961 agcctgcagg acgcccagag actgttcctg atgtctgaag ccgtgcagcc cgtgacacct
     4021 gagccttgtg tgacacagga tagcgtgcgg ttctcccacc tggtggtgga tctggtgcag
     4081 gccaaagaca ccctgtacca cgtgctgtac atcggcacag agagcggcac aatcctgaag
     4141 gccctgtcta ccgccagcag atccctgcac ggatgctacc tggaagaact gcacgtgctg
     4201 ccacctggca gaagagagcc tctgagatcc ctgagaatcc tgcacagcgc cagggctctg
     4261 tttgtgggac tgagagatgg cgtgctgaga gtgcccctgg aaagatgtgc cgcctacaga
     4321 tcacagggcg cctgtctggg agctagagat ccttactgtg gctgggacgg caagcagcag
     4381 agatgtagca ccctggaaga ttccagcaac atgagcctgt ggacccagaa catcaccgcc
     4441 tgtcctgtgc ggaacgtgac acgagatggc ggctttggac cttggagtcc ttggcagcct
     4501 tgcgagcatc tggacggcga taatagcggc tcctgcctgt gtagagccag aagctgcgat
     4561 agccccagac ctagatgtgg cggcctggat tgtctgggcc ctgccatcca tatcgccaac
     4621 tgctccagaa acggcgcctg gacaccttgg tcatcttggg ccctgtgtag cacctcttgc
     4681 ggcatcggat ttcaagtccg gcagcggagc tgcagcaacc ctgctcctag acatggcggc
     4741 agaatctgcg tgggcaagtc ccgggaagag agattctgca acgagaacac cccttgtcca
     4801 gtgcctatct tctgggcctc ttggggcagc tggtccaagt gcagctctaa ttgtggcggc
     4861 ggaatgcaga gcagaagaag ggcctgcgag aacggcaata gctgtctcgg atgcggcgtg
     4921 gaattcaaga cctgcaatcc tgagggctgc cccgaagtgc ggagaaacac accttggact
     4981 ccatggctgc ccgtgaacgt tacacaaggc ggagccagac aagagcagcg gttccggttt
     5041 acctgtagag cccctctggc tgatcctcac ggcctgcagt tcggaagaag aagaaccgag
     5101 acacggacct gtcctgccga tggcagcggc tcttgtgata cagatgccct ggtggaagtg
     5161 ctgctgagaa gcggctctac aagccctcac acagtgtctg gtggatgggc tgcttgggga
     5221 ccttggtcca gctgtagcag agattgcgag ctgggcttca gagtgcggaa gcggacctgt
     5281 accaatccag agcctaggaa cggcggcctg ccatgtgttg gagatgccgc cgaataccag
     5341 gactgcaacc ctcaggcctg tccagttaga ggcgcctggt cttgttggac ctcttggagc
     5401 ccttgttccg cctcttgtgg cggaggccac taccagagaa caagaagctg cacaagcccc
     5461 gcaccttctc caggcgagga tatttgcctg ggcctgcata ctgaagaggc cctgtgcgca
     5521 acacaggctt gtccagaagg atggtcccct tggagcgagt ggtctaagtg cacagatgac
     5581 ggcgcccaga gcagatctag gcactgcgag gaactgctgc ctggatcttc tgcctgtgcc
     5641 ggcaattcta gccagagccg gccttgtcca tacagcgaga tcccagtgat cctgcctgcc
     5701 agctctatgg aagaggccac agattgcgcc ggcttcaacc tgatccatgg cgcgccggaa
     5761 gatcaagttg atccaagatt gattgatggc aagggcggat ctctggaggt gctgttccag
     5821 ggaccaggcg gaagcggcaa tcccggcggc gatctggccc cccagatgct gcgcgagctg
     5881 caggagacca atgccgccct gcaggacgtg cgcgagctgc tgcgccaccg cgtgaaggag
     5941 atcaccttcc tgaagaacac cgtgatggag tgcgacgcct gcggtatgca gccctctaga
     6001 ggctccggct ccatcatccc agttgaggag gagaacccgg acttctggaa ccgcgaggca
     6061 gccgaggccc tgggtgccgc caagaagctg cagcctgcac agacagccgc caagaacctc
     6121 atcatcttcc tgggcgatgg gatgggggtg tctacggtga cagctgccag gatcctaaaa
     6181 gggcagaaga aggacaaact ggggcctgag atacccctgg ccatggaccg cttcccatat
     6241 gtggctctgt ccaagacata caatgtagac aaacatgtgc cagacagtgg agccacagcc
     6301 acggcctacc tgtgcggggt caagggcaac ttccagacca ttggcttgag tgcagccgcc
     6361 cgctttaacc agtgcaacac gacacgcggc aacgaggtca tctccgtgat gaatcgggcc
     6421 aagaaagcag ggaagtcagt gggagtggta accaccacac gagtgcagca cgcctcgcca
     6481 gccggcacct acgcccacac ggtgaaccgc aactggtact cggacgccga cgtgcctgcc
     6541 tcggcccgcc aggaggggtg ccaggacatc gctacgcagc tcatctccaa catggacatt
     6601 gacgtgatcc taggtggagg ccgaaagtac atgtttcgca tgggaacccc agaccctgag
     6661 tacccagatg actacagcca aggtgggacc aggctggacg ggaagaatct ggtgcaggaa
     6721 tggctggcga agcgccaggg tgcccggtat gtgtggaacc gcactgagct catgcaggct
     6781 tccctggacc cgtctgtgac ccatctcatg ggtctctttg agcctggaga catgaaatac
     6841 gagatccacc gagactccac actggacccc tccctgatgg agatgacaga ggctgccctg
     6901 cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg tcgcatcgac
     6961 catggtcatc atgaaagcag ggcttaccgg gcactgactg agacgatcat gttcgacgac
     7021 gccattgaga gggcgggcca gctcaccagc gaggaggaca cgctgagcct cgtcactgcc
     7081 gaccactccc acgtcttctc cttcggaggc taccccctgc gagggagctc catcttcggg
     7141 ctggcccctg gcaaggcccg ggacaggaag gcctacacgg tcctcctata cggaaacggt
     7201 ccaggctatg tgctcaagga cggcgcccgg ccggatgtta ccgagagcga gagcgggagc
     7261 cccgagtatc ggcagcagtc agcagtgccc ctggacgaag agacccacgc aggcgaggac
     7321 gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca ggagcagacc
     7381 ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc cctacaccgc ctgcgacctg
     7441 gcgccccccg ccggcaccac cgacgccgcg cacccgggtg gcggaggact gaacgacatc
     7501 ttcgaggctc agaaaatcga atggcacgaa catcaccatc accatcacca tcaccattaa
     7561 ggccggcctg tagtcactgg cacaacgcgt tacccggtaa gccaatcggg tatacacggt
     7621 cgtcatactg cagacagggt tcttctactt tgcaagatag tcttgagtag taaaataaat
     7681 agatagagaa aatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     7741 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     7801 gaggaaattg catcacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7861 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7921 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     7981 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     8041 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     8101 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8161 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     8221 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     8281 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     8341 atcggcatag tataatacga caaggtgagg aactaaacca tgaccgagta caagcccacg
     8401 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     8461 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     8521 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     8581 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     8641 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     8701 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     8761 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     8821 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacatccgc gccccgcaac
     8881 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     8941 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac acgtgctacg agatttcgat
     9001 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     9061 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     9121 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     9181 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     9241 ataccgtcga cctctagcta gagcttggcg taatcatggt cattaccaat gcttaatcag
     9301 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9361 cgtgtagata actacgatac gggagggctt accatctggc cccagcgctg cgatgatacc
     9421 gcgagaacca cgctcaccgg ctccggattt atcagcaata aaccagccag ccggaagggc
     9481 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9541 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccatcgctac
     9601 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9661 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9721 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9781 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9841 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9901 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9961 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10021 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10081 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10141 catattcttc ctt
//
