LOCUS       sequence-304297-       10369 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10369)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NEO1-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6019..6042
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7702..7746
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7747..7773
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7913..7930)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7919..8030
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8148..8168)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8153..8482
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8333..8468
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8395..8414
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8530..8577
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8596..9195
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8596..8615)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9092..9112
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9325..9446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9362..9381)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9416..9435
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9499..10290,10291..10359))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10122..10141
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10360..10369,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccgc aagatctgga
     2761 tctgctcctc aatctcctgg cgcctccatc cggaccttca cacccttcta ctttctggtg
     2821 gaacccgtgg atacactgag cgtgcgggga agctctgtga tcctgaattg cagcgcctac
     2881 agcgagccct ctccaaagat cgagtggaag aaggacggca ccttcctgaa cctggtgtcc
     2941 gacgatagaa ggcagctgct gccagatggc agcctgttca tcagcaacgt ggtgcacagc
     3001 aagcacaaca agcccgacga gggctactat cagtgtgtgg ccactgtgga aagcctgggc
     3061 accatcatca gcagaaccgc caaactgatt gtggccggac tgcccagatt cacctctcag
     3121 cctgagcctt cttctgtgta cgccggcaac aacgccatcc tgaactgcga agtgaacgcc
     3181 gacctggtgc ctttcgtcag atgggagcag aacagacagc ccctgctgct ggacgacaga
     3241 gtgattaagc tgcctagcgg catgctggtc atctccaatg ctacagaagg cgacggcggc
     3301 ctgtacagat gtgtggttga atctggcggc cctcctaagt acagcgacga ggtggaactg
     3361 aaggtgctgc ccgatcctga agtgatcagc gatctggtgt tcctgaagca gccatctcca
     3421 ctcgtcagag tgatcggcca ggatgtggtg ctgccttgtg ttgcctctgg cctgcctaca
     3481 cctaccatca agtggatgaa gaacgaggaa gccctggaca ccgagagcag cgaaagactg
     3541 gttctgcttg ccggcggaag cctggaaatc tccgatgtga cagaggacga cgccggcacc
     3601 tatttctgta tcgccgacaa cggcaacgag acaattgagg cccaggccga actgacagtg
     3661 caggcccagc ctgagtttct gaaacagccc accaacatct acgcccacga gagcatggac
     3721 atcgtgttcg agtgtgaagt gaccggcaag cccacaccta ccgtgaaatg ggtcaagaac
     3781 ggcgacatgg tcatccccag cgactacttc aagatcgtga aagagcacaa tctgcaggtc
     3841 ctgggactcg tgaagtccga tgagggcttt taccagtgca ttgccgagaa cgacgtgggc
     3901 aatgctcaag ccggcgctca gctgatcatt ctggaacatg cccctgccac aacaggccct
     3961 ttgccttctg ctcctagaga tgtggtggcc agcctggtgt ctacccggtt tatcaagctg
     4021 acatggcgga cccctgccag cgatcctcat ggcgataatc tgacctacag cgtgttctac
     4081 accaaagagg gaatcgcccg cgagagagtg gaaaacacat ctcaccctgg cgagatgcaa
     4141 gtgaccatcc agaatctgat gcccgccacc gtgtacatct ttagagtgat ggcccagaac
     4201 aagcacggca gcggagaatc tagcgcccct ctgagagttg agacacagcc tgaagttcag
     4261 ctgcccggac ctgctccaaa tctgagagcc tatgccgcct ctcctaccag catcaccgtt
     4321 acctgggaga cacctgtgtc cggcaacggc gagatccaga actacaagct gtactacatg
     4381 gaaaagggca ccgacaaaga acaggacgtg gacgtgtcca gccacagcta caccatcaac
     4441 ggcctgaaga agtacaccga gtatagcttc agagtggtcg cctacaacaa acacggccct
     4501 ggcgttagca cacctgatgt ggccgtcaga accctgtctg atgtgccatc tgccgctcct
     4561 cagaacctga gcctggaagt gcggaacagc aagagcatca tgatccactg gcagcctcca
     4621 gcaccagcca cacagaatgg ccagatcacc ggctacaaga tccggtacag aaaggccagc
     4681 cggaagtccg acgtgaccga aacactggtg tctggcacac agctgagcca gctgatcgaa
     4741 ggcctggata gaggcaccga gtacaatttc agagtcgccg ctctgaccat caatggcaca
     4801 ggccctgcta cagattggct gtccgccgag acattcgaga gcgacctgga tgagacaaga
     4861 gtgcctgagg tgccaagctc tctgcatgtg cggcctctcg tgacctctat cgtggtgtct
     4921 tggacccctc ctgagaacca gaatatcgtc gtgcggggct atgccatcgg ctacggaatt
     4981 ggatctcccc acgcacagac aatcaaggtg gactacaagc agcggtacta cacaatcgag
     5041 aatctggacc ccagcagcca ctacgtgatc accctgaagg cctttaacaa cgtcggcgag
     5101 ggcatccctc tgtacgagtc tgctgtgacc agacctcaca ccgatacaag cgaggtggac
     5161 ctgttcgtga tcaacgcccc ttacacaccc gtgcctgatc ctacaccaat gatgcctcca
     5221 gtgggcgtgc aggccagcat cctgtctcac gataccatca gaatcacctg ggccgacaac
     5281 agcctgccta agcaccagaa gatcaccgac agccggtact ataccgtgcg ctggaaaaca
     5341 aacatccccg ccaacaccaa gtacaagaac gccaacgcca ccacactgag ctacctggtt
     5401 acaggcctga agcctaacac actgtatgag ttcagcgtga tggtcaccaa gggcagaaga
     5461 tccagcacct ggtctatgac agcccacggc accacattcg agctggtgcc tacaagccct
     5521 ccaaaggatg tgaccgtggt gtccaaagaa ggcaagccta agaccatcat cgtgaattgg
     5581 cagccaccta gcgaggccaa cggcaagatc acaggctata tcatctacta cagcaccgac
     5641 gtgaacgctg agatccacga ctgggtcatc gagcctgtcg tgggcaacag actgacccac
     5701 cagatccaag agctgaccct ggataccccg tactacttta agattcaggc ccggaactcc
     5761 aaaggcatgg gccctatgtc tgaggccgtg cagtttagaa cccctaaggc cgacagctcc
     5821 gacaagatgc ctaatgatca ggccagcggc agcggcggaa aaggatcaag acttcctgat
     5881 ctgggctccg attacaagcc tcctatgagc ggcagcaata gccctcacgg atctcccacc
     5941 tctccactgg acagcaacat gcttggcgcg ccggaagatc aagttgatcc aagattgatt
     6001 gatggcaagg gcggatctct ggaggtgctg ttccagggac caggcggaag cggcaatccc
     6061 ggcggcgatc tggcccccca gatgctgcgc gagctgcagg agaccaatgc cgccctgcag
     6121 gacgtgcgcg agctgctgcg ccaccgcgtg aaggagatca ccttcctgaa gaacaccgtg
     6181 atggagtgcg acgcctgcgg tatgcagccc tctagaggct ccggctccat catcccagtt
     6241 gaggaggaga acccggactt ctggaaccgc gaggcagccg aggccctggg tgccgccaag
     6301 aagctgcagc ctgcacagac agccgccaag aacctcatca tcttcctggg cgatgggatg
     6361 ggggtgtcta cggtgacagc tgccaggatc ctaaaagggc agaagaagga caaactgggg
     6421 cctgagatac ccctggccat ggaccgcttc ccatatgtgg ctctgtccaa gacatacaat
     6481 gtagacaaac atgtgccaga cagtggagcc acagccacgg cctacctgtg cggggtcaag
     6541 ggcaacttcc agaccattgg cttgagtgca gccgcccgct ttaaccagtg caacacgaca
     6601 cgcggcaacg aggtcatctc cgtgatgaat cgggccaaga aagcagggaa gtcagtggga
     6661 gtggtaacca ccacacgagt gcagcacgcc tcgccagccg gcacctacgc ccacacggtg
     6721 aaccgcaact ggtactcgga cgccgacgtg cctgcctcgg cccgccagga ggggtgccag
     6781 gacatcgcta cgcagctcat ctccaacatg gacattgacg tgatcctagg tggaggccga
     6841 aagtacatgt ttcgcatggg aaccccagac cctgagtacc cagatgacta cagccaaggt
     6901 gggaccaggc tggacgggaa gaatctggtg caggaatggc tggcgaagcg ccagggtgcc
     6961 cggtatgtgt ggaaccgcac tgagctcatg caggcttccc tggacccgtc tgtgacccat
     7021 ctcatgggtc tctttgagcc tggagacatg aaatacgaga tccaccgaga ctccacactg
     7081 gacccctccc tgatggagat gacagaggct gccctgcgcc tgctgagcag gaacccccgc
     7141 ggcttcttcc tcttcgtgga gggtggtcgc atcgaccatg gtcatcatga aagcagggct
     7201 taccgggcac tgactgagac gatcatgttc gacgacgcca ttgagagggc gggccagctc
     7261 accagcgagg aggacacgct gagcctcgtc actgccgacc actcccacgt cttctccttc
     7321 ggaggctacc ccctgcgagg gagctccatc ttcgggctgg cccctggcaa ggcccgggac
     7381 aggaaggcct acacggtcct cctatacgga aacggtccag gctatgtgct caaggacggc
     7441 gcccggccgg atgttaccga gagcgagagc gggagccccg agtatcggca gcagtcagca
     7501 gtgcccctgg acgaagagac ccacgcaggc gaggacgtgg cggtgttcgc gcgcggcccg
     7561 caggcgcacc tggttcacgg cgtgcaggag cagaccttca tagcgcacgt catggccttc
     7621 gccgcctgcc tggagcccta caccgcctgc gacctggcgc cccccgccgg caccaccgac
     7681 gccgcgcacc cgggtggcgg aggactgaac gacatcttcg aggctcagaa aatcgaatgg
     7741 cacgaacatc accatcacca tcaccatcac cattaaggcc ggcctgtagt cactggcaca
     7801 acgcgttacc cggtaagcca atcgggtata cacggtcgtc atactgcaga cagggttctt
     7861 ctactttgca agatagtctt gagtagtaaa ataaatagat agagaaaatc agcctcgact
     7921 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     7981 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc acaacactca
     8041 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     8101 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     8161 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8221 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8281 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     8341 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8401 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     8461 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     8521 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     8581 gtgaggaact aaaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     8641 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     8701 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     8761 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     8821 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     8881 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     8941 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     9001 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     9061 gtgcccgcct tcctggagac atccgcgccc cgcaacctcc ccttctacga gcggctcggc
     9121 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     9181 aagcccggtg cctgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     9241 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     9301 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     9361 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9421 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     9481 ttggcgtaat catggtcatt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9541 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9601 gggcttacca tctggcccca gcgctgcgat gataccgcga gaaccacgct caccggctcc
     9661 ggatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9721 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9781 agttaatagt ttgcgcaacg ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc
     9841 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9901 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9961 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    10021 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    10081 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    10141 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    10201 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    10261 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10321 aaagggaata agggcgacac ggaaatgttg aatactcata ttcttcctt
//
