LOCUS       sequence-304650-        9749 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Overexpression of human REV1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Arbab M, Shen MW, Mok B, Wilson C, Matuszek Z, Cassa CA, Liu DR
  TITLE     Determinants of Base Editing Outcomes from Target Library Analysis 
            and Machine Learning.
  JOURNAL   Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 
            10.1016/j.cell.2020.05.037.
  PUBMED    32533916
REFERENCE   2  (bases 1 to 9749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037."
COMMENT     Sequence Label: p2T-CAG-REV1-p2A-GFP-PuroR
FEATURES             Location/Qualifiers
     source          1..9749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      878..887
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(893..926)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        941..1320
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1322..1599
                     /label=chicken beta-actin promoter
     intron          1600..2616
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2540..2562
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2624..2643
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             5001..5057
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      5067..5076
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5073..5075,5076..5078,5079..5792)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5073..5075,5076..5078,5079..5789)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5118..5139)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5379..5398)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5726..5747
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5837..5971
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5861..5880)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5915..5934
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6201..6800)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6284..6304)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6781..6800
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6893..7209)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6907..7042
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7279..7299
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(7565..7585)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7565..7583)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7604..7620)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7604..7620)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7617..7639)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7628..7644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7652..7658,7659..7676,7677..7682))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7697..7718
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7835..7852)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8006..8594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8086..8105)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8765..9556,9557..9625))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9388..9407
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9626..9730)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc nggcgggtgt ggtggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccggcatatg gttcttgaca gaggtgtaaa aagtactcaa aaattttact caagtgaaag
      721 tacaagtact tagggaaaat tttactcaat taaaagtaaa agtatctggc tagaatctta
      781 cttgagtaaa agtaaaaaag tactccatta aaattgtact tgagtattaa ggaagtaaaa
      841 gtaaaagcaa gaaagatcga tctcgaagga tctggaggcc accatggtgt cgataacttc
      901 gtatagcata cattatacga agttatcgat aagcttgctc gacattgatt attgactagt
      961 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     1021 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     1081 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     1141 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     1201 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     1261 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     1321 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     1381 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
     1441 gggcgcgcgc crggsggggs ggggsggggs grggggsggg gsggggsgag gcggagaggt
     1501 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     1561 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
     1621 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
     1681 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     1741 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     1801 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     1861 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
     1921 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     1981 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     2041 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
     2101 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
     2161 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     2221 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     2281 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     2341 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     2401 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     2461 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     2521 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct
     2581 gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
     2641 gtgctgtctc atcattttgg caaagaattc ctcgagcggc cgccagtgtg atggatatcg
     2701 gatccgccac catgtacaat gctgttggca tctcaaccac tgtggagcca cttcaggagc
     2761 atggaattgg tcaattggga attgggtggc aacttggcat taagaacgga atgttttttg
     2821 ggcatgctaa acaactatgt cctaatcttc aagctgttcc atacgatttt catgcatata
     2881 aggaagtcgc acaaacattg tatgaaacat tggcaagcta cactcataac attgaagctg
     2941 tcagttgtga tgaagcgctg gtagacatta ccgaaatcct tgcagagacc aaacttactc
     3001 ctgatgaatt tgcaaatgct gttcgtatgg aaatcaaaga ccagacgaaa tgtgctgcct
     3061 ctgttggaat tggttctaat attctcctgg ctagaatggc aactagaaaa gcaaaaccag
     3121 atgggcagta ccacctaaaa ccagaagaag tagatgattt tatcagaggc cagctagtga
     3181 ccaatctacc aggagttgga cattcaatgg aatctaagtt ggcatctttg ggaattaaaa
     3241 cttgtggaga cttgcagtat atgaccatgg caaaactcca aaaagaattt ggtcccaaaa
     3301 caggtcagat gctttatagg ttctgccgtg gcttggatga tagaccagtt cgaactgaaa
     3361 aggaaagaaa atctgtttca gctgagatca actatggaat aaggtttact cagccaaaag
     3421 aggcagaagc ttttcttctg agtctttcag aagaaattca aagaagacta gaagccactg
     3481 gcatgaaggg taaacgtcta actctcaaaa tcatggtacg aaagcctggg gctcctgtag
     3541 aaactgcaaa atttggaggc catggaattt gtgataacat tgccaggact gtaactcttg
     3601 accaggcaac agataatgca aaaataattg gaaaggcgat gctaaacatg tttcatacaa
     3661 tgaaactaaa tatatcagat atgagagggg ttgggattca cgtgaatcag ttggttccaa
     3721 ctaatctgaa cccttccaca tgtcccagtc gcccatcagt tcagtcaagc cactttccta
     3781 gtgggtcata ctctgtccgt gatgtcttcc aagttcagaa agctaagaaa tccaccgaag
     3841 aggagcacaa agaagtattt cgggctgctg tggatctgga aatatcatct gcttctagaa
     3901 cttgcacttt cttgccacct tttcctgcac atctgccgac cagtcctgat actaacaagg
     3961 ctgagtcttc agggaaatgg aatggtctac atactcctgt cagtgtgcag tcgagactta
     4021 acctgagtat agaggtcccg tcaccttccc agctggatca gtctgtttta gaagcacttc
     4081 cacctgatct ccgggaacaa gtagagcaag tctgtgctgt ccagcaagca gagtcacatg
     4141 gcgacaaaaa gaaagaacca gtaaatggct gtaatacagg aattttgcca caaccagttg
     4201 ggacagtctt gttgcaaata ccagaacctc aagaatcgaa cagtgacgca ggaataaatt
     4261 taatagccct tccagcattt tcacaggtgg accctgaggt atttgctgcc cttcctgctg
     4321 aacttcagag ggagctgaaa gcagcgtatg atcaaagaca aaggcagggc gagaacagca
     4381 ctcaccagca gtcagccagc gcatctgtgc caaagaatcc tttacttcat ctaaaggcag
     4441 cagtgaaaga aaagaaaaga aacaagaaga aaaaaaccat tggttcacca aaaaggattc
     4501 agagtccttt gaataacaag ctgcttaaca gtcctgcaaa aactctgcca ggggcctgtg
     4561 gcagtcccca gaagttaatt gatgggtttc taaaacatga aggacctcct gcagagaaac
     4621 ccctggaaga actctctgct tctacttcag gtgtgccagg cctttctagt ttgcagtctg
     4681 acccagctgg ctgtgtgaga cctccagcac ccaatctagc tggagctgtt gaattcaatg
     4741 atgtgaagac cttgctcaga gaatggataa ctacaatttc agatccaatg gaagaagaca
     4801 ttctccaagt tgtgaaatac tgtactgatc taatagaaga aaaagatttg gaaaaactgg
     4861 atctagttat aaaatacatg aaaaggctga tgcagcaatc ggtggaatcg gtttggaata
     4921 tggcatttga ctttattctt gacaatgtcc aggtggtttt acaacaaact tatggaagca
     4981 cattaaaagt tacagctagc gctactaact tcagcctgct gaagcaggct ggagacgtgg
     5041 aggagaaccc tggacctgga ccggtcgcca ccatggtgag caagggcgag gagctgttca
     5101 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     5161 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     5221 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     5281 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     5341 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     5401 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     5461 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     5521 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     5581 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     5641 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     5701 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     5761 tcactctcgg catggacgag ctgtacaagt aaagcggccg ccaccgcggt ggagctcgaa
     5821 ttaattcatc gatgatgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     5881 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     5941 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     6001 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     6061 tatggctgat tatgatcctc tagagtcggt gggcctcggg ggcgggtgcg gggtcggcgg
     6121 ggccgccccg ggtggcttcg gtcggagcca tggggtcgtg cgctcctttc ggtcgggcgc
     6181 tgcgggtcgt ggggcgggcg tcaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt
     6241 ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg
     6301 ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact
     6361 ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gacgccgacg
     6421 gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt
     6481 tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac
     6541 accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc
     6601 gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg
     6661 gtgacccgct cgatgtggcg gtccgggtcg acggtgtggc gcgtggcggg gtagtcggcg
     6721 aacgcggcgg cgagggtgcg tacggcccgg gggacgtcgt cgcgggtggc gaggcgcacc
     6781 gtgggcttgt actcggtcat ggaaggtcgt ctccttgtga ggggtcaggg gcgtgggtca
     6841 ggggatggtg gcggcaccgg tcgtggcggc cgacctgcag gcatgcaagc tttttgcaaa
     6901 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     6961 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     7021 tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat
     7081 tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg
     7141 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     7201 ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     7261 agcctgggga ctttccacac cctaactgac acacattcca cagaattcaa gtgatctcca
     7321 aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta cttttttttc
     7381 taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact caagtaaagt
     7441 aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc aagtacttta
     7501 cacctctggt tcttgacccc ctaccttcag caagcccagc agatccgagc tccagctttt
     7561 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     7621 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7681 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7741 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7801 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7861 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7921 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7981 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     8041 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     8101 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     8161 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     8221 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     8281 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     8341 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     8401 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     8461 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8521 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8581 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8641 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8701 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8761 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8821 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8881 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8941 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     9001 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     9061 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     9121 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     9181 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     9241 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     9301 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     9361 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     9421 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9481 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9541 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9601 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9661 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9721 gggttccgcg cacatttccc cgaaaagtg
//
