LOCUS       sequence-304689-       10322 bp    DNA     circular SYN 14-APR-2025
DEFINITION  A-to-G base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Arbab M, Shen MW, Mok B, Wilson C, Matuszek Z, Cassa CA, Liu DR
  TITLE     Determinants of Base Editing Outcomes from Target Library Analysis 
            and Machine Learning.
  JOURNAL   Cell. 2020 Jun 9. pii: S0092-8674(20)30632-2. doi: 
            10.1016/j.cell.2020.05.037.
  PUBMED    32533916
REFERENCE   2  (bases 1 to 10322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jun 9. pii: S0092-8674(20)30632-2. doi: 10.1016/j.cell.2020.05.037."
COMMENT     Sequence Label: p2T-CMV-ABEmax-BlastR
FEATURES             Location/Qualifiers
     source          1..10322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             116..136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1325..1348,1349..1351,1352..5425)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1325..5425
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5468..5488
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5497..5514
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5537..5554)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5543..5767
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5838..5854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5838..5854)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5851..5873)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5862..5878
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5886..5892,5893..5910,5911..5916))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5931..5952
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    6069..6190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6080..6099)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6134..6153
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6348..6746)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6765..6812)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6860..7189)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6874..7009
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6928..6947)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7174..7194
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7573..8161)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7653..7672)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8332..9123,9124..9192))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8955..8974
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9193..9297)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9376..9395)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9745..10124
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(10125..10322,1..6)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10279..10299
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagagatccg cggccgctaa tacgactcac
       61 tatagggaga gccgccacca tgaaacggac agccgacgga agcgagttcg agtcaccaaa
      121 gaagaagcgg aaagtctctg aagtcgagtt tagccacgag tattggatga ggcacgcact
      181 gaccctggca aagcgagcat gggatgaaag agaagtcccc gtgggcgccg tgctggtgca
      241 caacaataga gtgatcggag agggatggaa caggccaatc ggccgccacg accctaccgc
      301 acacgcagag atcatggcac tgaggcaggg aggcctggtc atgcagaatt accgcctgat
      361 cgatgccacc ctgtatgtga cactggagcc atgcgtgatg tgcgcaggag caatgatcca
      421 cagcaggatc ggaagagtgg tgttcggagc acgggacgcc aagaccggcg cagcaggctc
      481 cctgatggat gtgctgcacc accccggcat gaaccaccgg gtggagatca cagagggaat
      541 cctggcagac gagtgcgccg ccctgctgag cgatttcttt agaatgcgga gacaggagat
      601 caaggcccag aagaaggcac agagctccac cgactctgga ggatctagcg gaggatcctc
      661 tggaagcgag acaccaggca caagcgagtc cgccacacca gagagctccg gcggctcctc
      721 cggaggatcc tctgaggtgg agttttccca cgagtactgg atgagacatg ccctgaccct
      781 ggccaagagg gcacgcgatg agagggaggt gcctgtggga gccgtgctgg tgctgaacaa
      841 tagagtgatc ggcgagggct ggaacagagc catcggcctg cacgacccaa cagcccatgc
      901 cgaaattatg gccctgagac agggcggcct ggtcatgcag aactacagac tgattgacgc
      961 caccctgtac gtgacattcg agccttgcgt gatgtgcgcc ggcgccatga tccactctag
     1021 gatcggccgc gtggtgtttg gcgtgaggaa cgcaaaaacc ggcgccgcag gctccctgat
     1081 ggacgtgctg cactaccccg gcatgaatca ccgcgtcgaa attaccgagg gaatcctggc
     1141 agatgaatgt gccgccctgc tgtgctattt ctttcggatg cctagacagg tgttcaatgc
     1201 tcagaagaag gcccagagct ccaccgactc cggaggatct agcggaggct cctctggctc
     1261 tgagacacct ggcacaagcg agagcgcaac acctgaaagc agcgggggca gcagcggggg
     1321 gtcagacaag aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt
     1381 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg
     1441 gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga
     1501 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg
     1561 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag
     1621 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg
     1681 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa
     1741 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca
     1801 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga
     1861 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc
     1921 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag
     1981 acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa
     2041 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga
     2101 ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc
     2161 ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat
     2221 cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc
     2281 tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg
     2341 gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc
     2401 cggctacatt gacggcggag ccagccagga agagttctac aagttcatca agcccatcct
     2461 ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg
     2521 gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca
     2581 cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat
     2641 cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag
     2701 cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga
     2761 agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa
     2821 gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt
     2881 gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct
     2941 gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac
     3001 cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat
     3061 ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat
     3121 tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt
     3181 gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc
     3241 ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg
     3301 caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct
     3361 ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga
     3421 cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct
     3481 gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac
     3541 agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt
     3601 gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag
     3661 aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc
     3721 cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg
     3781 ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg atgtggacca
     3841 tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag
     3901 cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa
     3961 gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct
     4021 gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca
     4081 gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa
     4141 cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc
     4201 caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa
     4261 ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa
     4321 gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa
     4381 gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag
     4441 caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg
     4501 gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt
     4561 tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt
     4621 gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat
     4681 cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc
     4741 ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt
     4801 gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga
     4861 ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa
     4921 gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact
     4981 gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag
     5041 ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga
     5101 acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt
     5161 gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa
     5221 gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc
     5281 ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa
     5341 agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat
     5401 cgacctgtct cagctgggag gtgactctgg cggctcaaaa agaaccgccg acggcagcga
     5461 attcgagccc aagaagaaga ggaaagtcta accggtcatc atcaccatca ccattgagtt
     5521 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     5581 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     5641 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     5701 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     5761 tctatggctt ctgaggcgga aagaaccagc tggggctcga taccgtcgac ctctagctag
     5821 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5881 ccacacaaca tacgagccgg aagcataaag tgtaaagcct agggtgccta atgagtgagc
     5941 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     6001 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctac
     6061 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     6121 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     6181 taaacaagtt ggggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
     6241 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
     6301 cgaaatctcg tagcacgtgt cagtcctgct cctcggccac gaagtgctta gccctcccac
     6361 acataaccag agggcagcaa ttcacgaatc ccaactgccg tcggctgtcc atcactgtcc
     6421 ttcactatgg ctttgatccc aggatgcaga tcgagaagca cctgtcggca ccgtccgcag
     6481 gggctcaaga tgcccctgtt ctcatttccg atcgcgacga tacaagtcag gttgccagct
     6541 gccgcagcag cagcagtgcc cagcaccacg agttctgcac aaggtccccc agtaaaatga
     6601 tatacattga caccagtgaa gatgcggccg tcgctagaga gagctgcgct ggcgacgctg
     6661 tagtcttcag agatggggat gctgttgatt gtagccgttg ctctttcaat gagggtggat
     6721 tcttcttgag acaaaggctt ggccatggtt tagttcctca ccttgtcgta ttatactatg
     6781 ccgatatact atgccgatga ttaattgtca acacgtgctg atcagatccg aaaatggata
     6841 tacaagctcc cgggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     6901 tctggaatag ctcagaggca gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     6961 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     7021 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     7081 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     7141 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagaa
     7201 ttcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7261 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtaagtgatc
     7321 tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt
     7381 tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta
     7441 aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac
     7501 tttacacctc tgcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7561 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7621 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7681 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7741 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7801 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7861 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7921 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7981 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8041 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8101 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8161 agaagatcct ttgatctttt ctacggggtc tgacactcag tggaacgaaa actcacgtta
     8221 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8281 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8341 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8401 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8461 aatgataccg cgggacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8521 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8581 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8641 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8701 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8761 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8821 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8881 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8941 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9001 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9061 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9121 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9181 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9241 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9301 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc ccgatcccct
     9361 agggtcgact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     9421 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     9481 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     9541 cgatgtacgg gccagatata cgcgggtacc ggcatatggt tcttgacaga ggtgtaaaaa
     9601 gtactcaaaa attttactca agtgaaagta caagtactta gggaaaattt tactcaatta
     9661 aaagtaaaag tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa
     9721 attgtacttg agtattaacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     9781 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     9841 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     9901 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     9961 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
    10021 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
    10081 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
    10141 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
    10201 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
    10261 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
    10321 gc
//
