LOCUS       sequence-304734-        4398 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV2 TEV-NSP14
FEATURES             Location/Qualifiers
     source          1..4398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(57..143)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     192..214
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     206..223
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     207..223
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    239..338
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             338..358
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(1947..2046)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2063..2082)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2064..2082)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2087..2103)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2087..2103)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2534..3325
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3418..4006
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3907..3926
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4160..4177
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4336..4363)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt
       61 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc
      121 gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc
      181 atggatgttt tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggcccca
      241 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg
      301 cttttttata atgccaactt tgtacaaaaa agttggcgaa aacctgtact tccaaggcat
      361 ggctgagaac gtgaccggtc tgttcaagga ctgctctaag gtcatcactg gtctgcaccc
      421 aactcaggct ccaacccacc tgtctgtgga caccaagttc aagaccgaag gactgtgcgt
      481 ggacatcccc ggtatcccta aggacatgac ctaccgcagg ctgatctcca tgatgggctt
      541 caagatgaac taccaggtga acggataccc taacatgttc atcaccagag aggaagctat
      601 ccgccacgtg agggcctgga tcggtttcga cgtggaaggt tgccacgcta ccagagaagc
      661 tgtgggtacc aacctgccac tgcagctggg cttctctacc ggagtgaacc tggtggctgt
      721 gcctaccggc tacgtggaca ccccaaacaa caccgacttc tctagagtga gcgccaagcc
      781 ccctccagga gaccagttca agcacctgat ccccctgatg tacaagggtc tgccttggaa
      841 cgtggtgcgc atcaagatcg tgcagatgct gtccgacacc ctgaagaacc tgtctgacag
      901 ggtggtgttc gtgctgtggg ctcacggctt cgagctgacc agcatgaagt acttcgtgaa
      961 gatcggaccc gaacgtacct gctgcctgtg cgacagacgt gccacctgct tctccaccgc
     1021 ctctgacacc tacgcttgct ggcaccactc catcggattc gactacgtgt acaacccttt
     1081 catgatcgac gtgcagcagt ggggattcac cggtaacctg cagtccaacc acgacctgta
     1141 ctgccaggtg cacggtaacg cccacgtggc ttcttgcgac gctatcatga ccagatgcct
     1201 ggccgtgcac gagtgcttcg tgaagcgtgt ggactggacc atcgagtacc ctatcatcgg
     1261 cgacgaactg aagatcaacg ccgcttgcag aaaggtgcag cacatggtgg tgaaggccgc
     1321 tctgctggct gacaagttcc cagtgctgca cgacatcgga aacccaaagg ctatcaagtg
     1381 cgtgccccag gccgacgtgg agtggaagtt ctacgacgct cagccctgct ccgacaaggc
     1441 ctacaagatc gaggaactgt tctactctta cgctacccac agcgacaagt tcaccgacgg
     1501 cgtgtgcctg ttctggaact gcaacgtgga caggtaccca gccaactcta tcgtgtgcag
     1561 attcgacacc cgtgtgctga gcaacctgaa cctgcccgga tgcgacggcg gatccctgta
     1621 cgtgaacaag cacgccttcc acacccctgc tttcgacaag tctgccttcg tgaacctgaa
     1681 gcagctgcct ttcttctact acagcgactc cccatgcgag agccacggca agcaggtggt
     1741 gtccgacatc gactacgtgc ccctgaagag cgccacctgc atcacccgct gcaacctggg
     1801 cggcgccgtg tgcagacacc acgccaacga atacaggctg tacctggacg cttacaacat
     1861 gatgatcagc gccggcttct ccctgtgggt gtacaagcag ttcgacacct acaacctgtg
     1921 gaacaccttc accaggctgc agtaataccc aactttcttg tacaaagttg gcattataag
     1981 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     2041 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     2101 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     2161 atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa
     2221 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc
     2281 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     2341 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     2401 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     2461 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     2521 aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     2581 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     2641 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     2701 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     2761 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     2821 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     2881 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     2941 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     3001 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     3061 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     3121 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     3181 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     3241 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     3301 gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt
     3361 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3541 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3661 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3721 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3781 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3841 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3901 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3961 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4021 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4081 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4141 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     4201 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     4261 cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga
     4321 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg
     4381 gcagtttatg gcgggcgt
//
