LOCUS       sequence-305079-        3673 bp    DNA     circular SYN 15-APR-2025
DEFINITION  High Copy number plasmid containing smCBA insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   de Moura Gomes A, L Petkau T, J Korecki A, Fornes O, Galvan A, Lu G,
            M Hill A, Ling Lam S, Yao A, A Farkas R, W Wasserman W, Smith Y, M 
            Simpson E, R Leavitt B
  TITLE     New MiniPromoter Ple389 (ADORA2A) drives selective expression in 
            medium spiny neurons in mice and non-human primates.
  JOURNAL   Sci Rep. 2024 Nov 15;14(1):28194. doi: 10.1038/s41598-024-79004-y.
  PUBMED    39548191
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-11-15"; volume: "14(1):28194. doi"; pages: " 
            10.1038/s41598-024-79004-y"
COMMENT     Sequence Label: pEMS2075
FEATURES             Location/Qualifiers
     source          1..3673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(461..480)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        complement(737..1014)
                     /label=chicken beta-actin promoter
     enhancer        1016..1319
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(1452..1468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1452..1468)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1465..1487)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1476..1492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1500..1506,1507..1524,1525..1530))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1545..1566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1683..1700)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1854..2442)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1934..1953)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2613..3404,3405..3473))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3236..3255
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3474..3578)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3646..3664
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tggcgcgcct ttgccaaaat gatgagacag cacaataacc agcacgttgc
      481 ccaggagctg taggaaaaag aagaaggcat gaacatggtt agcagaggct ctagctcccg
      541 gagcccctca aggctttcac gcagccacag aaaagaaaca agccgtcatt aaaccaagcg
      601 ctaattacag cccggaggag aagggccgtc ccgcccgctc acctgtggga gtaacgcggt
      661 cagtcagagc cggggcgggc ggcgcgaggc ggcggcggag cggggcacgg ggcgaaggca
      721 gcgtcgcagc gactcccgcc cgccgcgcgc ttcgcttttt atagggccgc cgccgccgcc
      781 gcctcgccat aaaaggaaac tttcggagcg cgccgctctg attggctgcc gccgcacctc
      841 tccgcctcgc cccgccccgc ccctcgcccc gccccgcccc gcctggcgcg cgcccccccc
      901 ccccccccgc ccccatcgct gcacaaaata attaaaaaat aaataaatac aaaattgggg
      961 gtggggaggg gggggagatg gggagagtga agcagaacgt ggggctcacc tcgaccatgg
     1021 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     1081 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     1141 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatag tccacccatt
     1201 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     1261 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     1321 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
     1381 ggtaccggcc ggccatcgga tcccgggccc gtcgactgca gaggcctgca tgcaagcttg
     1441 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     1501 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     1561 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     1621 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     1681 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1741 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1801 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1861 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1921 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1981 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2041 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2101 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2161 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2221 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2281 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2341 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2401 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     2461 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     2521 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     2581 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     2641 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     2701 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2761 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2821 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2881 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     2941 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3001 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     3061 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     3121 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     3181 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     3241 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     3301 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     3361 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     3421 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     3481 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     3541 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     3601 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     3661 aggccctttc gtc
//
