LOCUS       sequence-305337-         951 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_HA.AU1.NWS_NGFR
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             181..204
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgctacc catacgatgt gccagattac
      121 gctggaggag gcgggggagg ggacacctat cggtacattg gaggaggcgg gggagggaac
      181 tggtcccacc cccaattcga gaaacccaca ggcctgtaca cacacagcgg tgagtgctgc
      241 aaagcctgca acctgggcga gggtgtggcc cagccttgtg gagccaacca gaccgtgtgt
      301 gagccctgcc tggacagcgt gacgttctcc gacgtggtga gcgcgaccga gccgtgcaag
      361 ccgtgcaccg agtgcgtggg gctccagagc atgtcggcgc cgtgcgtgga ggccgacgac
      421 gccgtgtgcc gctgcgccta cggctactac caggatgaga cgactgggcg ctgcgaggcg
      481 tgccgcgtgt gcgaggcggg ctcgggcctc gtgttctcct gccaggacaa gcagaacacc
      541 gtgtgcgagg agtgccccga cggcacgtat tccgacgagg ccaaccacgt ggacccgtgc
      601 ctgccctgca ccgtgtgcga ggacaccgag cgccagctcc gcgagtgcac acgctgggcc
      661 gacgccgagt gcgaggagat ccctggccgt tggattacac ggtccacacc cccagagggc
      721 tcggacagca cagcccccag cacccaggag cctgaggcac ctccagaaca agacctcata
      781 gccagcacgg tggcaggtgt ggtgaccaca gtgatgggca gctcccagcc cgtggtgacc
      841 cgaggcacca ccgacaacct catccctgtc tattgctcca tcctggctgc tgtggttgtg
      901 ggccttgtgg cctacatagc cttcaagagg tggaacaggg gtatcctcta g
//
