LOCUS       sequence-305477-        1011 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_Tag.V5.S.Ollas_NGFR
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..138
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             157..201
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             220..261
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
ORIGIN
        1 atgggggcag gtgccaccgg ccgcgccatg gacgggccgc gcctgctgct gttgctgctt
       61 ctgggggtgt cccttggagg tgccaaggag gcatgcggca aaccaattcc aaacccactg
      121 ctgggactgg attccacagg aggaggcggg ggagggaagg agaccgccgc cgccaagttc
      181 gagaggcagc acatggacag cggaggaggc gggggaggga gcggcttcgc caacgagctg
      241 ggccccaggc tgatgggcaa gtgccccaca ggcctgtaca cacacagcgg tgagtgctgc
      301 aaagcctgca acctgggcga gggtgtggcc cagccttgtg gagccaacca gaccgtgtgt
      361 gagccctgcc tggacagcgt gacgttctcc gacgtggtga gcgcgaccga gccgtgcaag
      421 ccgtgcaccg agtgcgtggg gctccagagc atgtcggcgc cgtgcgtgga ggccgacgac
      481 gccgtgtgcc gctgcgccta cggctactac caggatgaga cgactgggcg ctgcgaggcg
      541 tgccgcgtgt gcgaggcggg ctcgggcctc gtgttctcct gccaggacaa gcagaacacc
      601 gtgtgcgagg agtgccccga cggcacgtat tccgacgagg ccaaccacgt ggacccgtgc
      661 ctgccctgca ccgtgtgcga ggacaccgag cgccagctcc gcgagtgcac acgctgggcc
      721 gacgccgagt gcgaggagat ccctggccgt tggattacac ggtccacacc cccagagggc
      781 tcggacagca cagcccccag cacccaggag cctgaggcac ctccagaaca agacctcata
      841 gccagcacgg tggcaggtgt ggtgaccaca gtgatgggca gctcccagcc cgtggtgacc
      901 cgaggcacca ccgacaacct catccctgtc tattgctcca tcctggctgc tgtggttgtg
      961 ggccttgtgg cctacatagc cttcaagagg tggaacaggg gtatcctcta g
//
