LOCUS       sequence-305607-         795 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Gateway destination clone of BCKDK (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-BCKDK-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(593..617)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             619..660
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gactttgtcg gcatcatctg tactcgtctc tcaccaaaga agattattga gaagtgggtg
       61 gactttgcca gacgcctgtg tgagcacaag tatggcaatg cgccccgtgt ccgcatcaat
      121 ggccatgtgg ctgcccggtt ccccttcatc cctatgccac tggactacat cctgccggag
      181 ctgctcaaga atgccatgag agccacaatg gagagtcacc tagacactcc ctacaatgtc
      241 ccagatgtgg tcatcaccat cgccaacaat gatgtcgatc tgatcatcag gatctcagac
      301 cgtggtggag gaatcgctca caaagatctg gaccgggtca tggactacca cttcactact
      361 gctgaggcca gcacacagga cccccggatc agccccctct ttggccatct ggacatgcat
      421 agtggcgccc agtcaggacc catgcacggc tttggcttcg ggttgcccac gtcacgggcc
      481 tacgcggagt acctcggtgg gtctctgcag ctgcagtccc tgcagggcat tggcacggac
      541 gtctacctgc ggctccgcca catcgatggc cgggaggaaa gcttccggat ctgcccaact
      601 ttcttgtaca aagtgggcgg caagcccatc cccaaccccc tgctgggcct ggacagcacc
      661 gctagcatcc cgctgctgaa cgctaaacag attctgggac agctggacgg cgggagcgtg
      721 gcagtcctgc ctgtggtcga ntccaccaat cagtacctgc tggatcgaat cggcgagctg
      781 aagagtgggg atgct
//
