LOCUS       sequence-305623-         851 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Gateway destination clone of PHKG1 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-PHKG1-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(645..669)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             671..712
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ccagctggag ccgggagaga ggctgcgaga ggtctgtggg acccccagtt acctggcccc
       61 tgagattatc gagtgctcca tgaatgagga ccacccgggc tacgggaaag aggtggacat
      121 gtggagcact ggcgtcatca tgtacacgct gctggccggc tccccgccct tctggcaccg
      181 gaagcagatg ctgatgctga ggatgatcat gagcggcaac taccagtttg gctcgcccga
      241 gtgggatgat tactcggaca ccgtgaagga cctggtctcc cgattcctgg tggtgcaacc
      301 ccagaaccgc tacacagcgg aagaggcctt ggcacacccc ttcttccagc agtacttggt
      361 ggaggaagtg cggcacttca gcccccgggg gaagttcaag gtgatcgctc tgaccgtgct
      421 ggcttcagtg cggatctact accagtaccg ccgggtgaag cctgtgaccc gggagatcgt
      481 catccgagac ccctatgccc tccggcctct gcgccggctc atcgacgcct acgctttccg
      541 aatctatggc cactgggtga agaaggggca gcagcagaac cgggcagccc ttttcgagaa
      601 cacacccaag gccgtgctcc tctccctggc cgaggaggac tacttgccaa ctttcttgta
      661 caaagtgggc ggcaagccca tccccaaccc cctgctgggc ctggacagca ccgctagcat
      721 cccgctgctg aacgctaaac agattctggg acagctggac ggcgggagcg tggcagtcct
      781 gcctgtggtc gactccacca atcagtacct gctggatcga atcggcgagc tgaagagtgg
      841 ggatgcttgc a
//
