LOCUS       sequence-305806-       10713 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting 
            NbPDS3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 10713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: pEE393
FEATURES             Location/Qualifiers
     source          1..10713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        1901..1976
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             2548..2565
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2600..2852
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2874..2898
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4199..4828
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5257..6330
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6396..6590
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6826..6848)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6934..7074
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6989..7008
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7089..7106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7260..7848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7340..7359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9273..10067)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10492..10516
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttc atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg
      121 cgataaagga aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc
      181 cccacccacg aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggataaaac attgcaccta
      361 tggtgttgcc ctggctgggg tatgtcagtg atcgcagtag aatgtactaa ttgacaagtt
      421 ggagaatacg gtagaacgtc cttatccaac acagccttta tccctctccc tgacgaggtt
      481 tttgtcagtg taatatttct ttttgaacta tccagcttag taccgtacgg gaaagtgact
      541 ggtgtgctta tctttgaaat gttactttgg gtttcggttc tttaggttag taagaaagca
      601 cttgtcttct catacaaagg aaaacctgag acgtatcgct tacgaaagta gcaatgaaag
      661 aaaggtggtg gttttaatcg ctaccgcaaa aacgatgggg tcgttttaat taacttctcc
      721 tacgcaagcg tctaaacgga cgttggggtt ttgctagttt ctttagagaa aactagctaa
      781 gtctttaatg ttatcattag agatggcata aatataatac ttgtgtctgc tgataagatc
      841 attttaattt ggacgattag acttgttgaa ctacaggtta ctgaatcact tgcgctaatc
      901 aacatgggag atatgtacga tgaatcattt gacaagtcgg gcggtcctgc tgacttgatg
      961 gacgattctt gggtggaatc agtttcgtgg aaagatctgt tgaagaagtt acacagcata
     1021 aaatttgcac tacagtctgg tagagatgag atcactgggt tactagcggc actgaataga
     1081 cagtgtcctt attcaccata tgagcagttt ccagataaga aggtgtattt ccttttagac
     1141 tcacgggcta acagtgctct tggtgtgatt cagaacgctt cagcgttcaa gagacgagct
     1201 gatgagaaga atgcagtggc gggtgttaca aatattcctg cgaatccaaa cacaacggtt
     1261 acgacgaacc aagggagtac tactactacc aaggcgaaca ctggctcgac tttggaagaa
     1321 gacttgtaca cttattacaa attcgatgat gcctctacag ctttccacaa atctctaact
     1381 tcgttagaga acatggagtt gaagagttat taccgaagga actttgagaa agtattcggg
     1441 attaagtttg gtggagcagc tgctagttca tctgcaccgc ctccagcgag tggaggtccg
     1501 atacgtccta atccctaggg atttaaggac gtgaactctg ttgagatctc tgtgaaattc
     1561 agagggtggg tgataccata ttcactgatg ccattagcga catctaaata gggctaattg
     1621 tgactaattt gagggaattt cctttaccat tgacgtcagt gtcgttggta gcatttgagt
     1681 ttcggagcat cttgttctgg ggtttcacac tatctttaga gaaagtgtta agttaattaa
     1741 gttatcttaa ttaagagcat aattatactg atttgtctct cgttgataga gtctatcatt
     1801 ctgttactaa aaatttgaca actcggtttg ctgacctact ggttactgta tcacttaccc
     1861 gagttaacga gtctagaaac ttggtagtag cgactccatg gttttagagc tagaaatagc
     1921 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctccc
     1981 tagtctataa atataagaga ccctcttata gtaagcagag ttgttggaga cgttcttgat
     2041 ccgtttaata gatcaatcac tctaaaggtt acttatggcc aaagtgccgg gcatgtcccg
     2101 aagacattaa actacggttc tttaagtaga tccgtgtctg aagttttagg ttcaatttaa
     2161 acctacgaga ttgacattct cgactgatct tgattgatcg gtaagtcttt tgtaatttaa
     2221 ttttcttttt gattttattt taaattgtta tctgtttctg tgtatagact gtttgagatc
     2281 ggcgtttggc cgactcattg tcttaccata ggggaacgga ctttgtttgt gttgttattt
     2341 tatttgtatt ttattaaaat tctcaacgat ctgaaaaagc ctcgcggcta agagattgtt
     2401 ggggggtgag taagtacttt taaagtgatg atggttacaa aggcaaaagg ggtaaaaccc
     2461 ctcgcctacg taagcgttat tacgcccgtc tgtacttata tcagtacact gacgagtccc
     2521 taaaggacga aacgggagaa cgctagccac caccaccacc accacgtgtg aattacaggt
     2581 gaccagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     2641 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     2701 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     2761 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     2821 atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta aactatcagt gtttgacagg
     2881 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
     2941 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
     3001 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
     3061 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
     3121 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
     3181 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
     3241 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
     3301 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
     3361 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
     3421 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
     3481 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
     3541 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
     3601 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
     3661 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
     3721 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
     3781 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
     3841 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
     3901 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
     3961 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg
     4021 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
     4081 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
     4141 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
     4201 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
     4261 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
     4321 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
     4381 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
     4441 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
     4501 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
     4561 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
     4621 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
     4681 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
     4741 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
     4801 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
     4861 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
     4921 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
     4981 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
     5041 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
     5101 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
     5161 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg
     5221 caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc
     5281 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
     5341 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
     5401 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg
     5461 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
     5521 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
     5581 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
     5641 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
     5701 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
     5761 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
     5821 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
     5881 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
     5941 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg
     6001 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
     6061 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
     6121 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
     6181 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
     6241 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
     6301 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
     6361 ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac
     6421 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
     6481 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
     6541 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
     6601 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
     6661 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
     6721 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
     6781 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     6841 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     6901 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     6961 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     7021 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     7081 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7141 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7201 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     7261 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     7321 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     7381 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     7441 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     7501 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     7561 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     7621 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7681 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7741 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7801 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7861 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gctgatgaat
     7921 cccctaatga tttttatcaa aatcattaag ttaaggtaga tacacatctt gtcatatgat
     7981 caaatggttt cgccaaaaat caataatcag acaacaaaat gtgcgaactc gatattttac
     8041 acgactctct ttaccaattc tgccccgaat tacacttaaa acgactcaac agcttaacgt
     8101 tggcttgcca cgccttactt gactgtaaaa ctctcactct taccgaactt ggccgtaacc
     8161 tgccaaccaa agcgagaaca aaacataaca tcaaacgaat cgaccgattg ttaggtaatc
     8221 gtcacctcca caaagagcga ctcgctgtat accgttggca tgctagcttt atctgttcgg
     8281 gcaatacgat gcccattgta cttgttgact ggtctgatat ccgtgagcaa aaacggctta
     8341 tggtattgcg agcttcagtc gcactacacg gtcgttctgt tactctttat gagaaagcgt
     8401 tcccgctttc agagcaatgt tcaaagaaag ctcatgacca atttctagcc gaccttgcga
     8461 gcattctacc gagtaacacc acaccgctca ttgtcagtga tgctggcttt aaagtgccat
     8521 ggtataaatc cgttgagaag ctgggttggt actggttaag tcgagtaaga ggaaaagtac
     8581 aatatgcaga cctaggagcg gaaaactgga aacctatcag caacttacat gatatgtcat
     8641 ctagtcactc aaagacttta ggctataaga ggctgactaa aagcaatcca atctcatgcc
     8701 aaattctatt gtataaatct cgctctaaag gccgaaaaaa tcagcgctcg acacggactc
     8761 attgtcacca cccgtcacct aaaatctact cagcgtcggc aaaggagcca tggattctag
     8821 caactaactt acctgttgaa attcgaacac ccaaacaact tgttaatatc tattcgaagc
     8881 gaatgcagat tgaagaaacc ttccgagact tgaaaagtcc tgcctacgga ctaggcctac
     8941 gccatagccg aacgagcagc tcagagcgtt ttgatatcat gctgctaatc gccctgatgc
     9001 ttcaactaac atgttggctt gcgggcgttc atgctcagaa acaaggttgg gacaagcact
     9061 tccaggctaa cacagtcaga aatcgaaacg tactctcaac agttcgctta ggcatggaag
     9121 ttttgcggca ttctggctac acaataacaa gggaagactc actcgtggct gcaaccctgc
     9181 ttactcaaaa tctattcaca catggttacg ttttggggaa attatgaggg gatctctcag
     9241 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa
     9301 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     9361 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
     9421 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
     9481 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
     9541 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
     9601 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
     9661 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
     9721 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
     9781 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
     9841 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
     9901 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
     9961 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
    10021 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
    10081 cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
    10141 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
    10201 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
    10261 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
    10321 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
    10381 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
    10441 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
    10501 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
    10561 aatgtactga attaacgccg aattaattcc taggccacca tgttgggccc ggcgcgccaa
    10621 gcttgcatgc ctgcaggtca acatggtgga gcacgacact ctcgtctact ccaagaatat
    10681 caaagataca gtctcagaag accagagggc tat
//
