LOCUS       sequence-305881-       11213 bp    DNA     circular SYN 14-APR-2025
DEFINITION  T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented 
            gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11213)
  AUTHORS   Ellison EE, Nagalakshmi U, Gamo ME, Huang PJ, Dinesh-Kumar S, Voytas
            DF
  TITLE     Multiplexed heritable gene editing using RNA viruses and mobile 
            single guide RNAs.
  JOURNAL   Nat Plants. 2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y.
  PUBMED    32483329
REFERENCE   2  (bases 1 to 11213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2020 Jun 1. pii: 10.1038/s41477-020-0670-y. doi: 
            10.1038/s41477-020-0670-y."
COMMENT     Sequence Label: pEE531
FEATURES             Location/Qualifiers
     source          1..11213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        2327..2402
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        2557..2632
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        2787..2862
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             3434..3451
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3486..3738
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3760..3784
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5085..5714
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6143..7216
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7282..7476
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7712..7734)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7820..7960
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7875..7894
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7975..7992)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8146..8734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8226..8245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9868..10662)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11087..11111
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg gagcatcttg ttctggggtt tcacactatc tttagagaaa gtgttaagtt
     2161 aattaagtta tcttaattaa gagcataatt atactgattt gtctctcgtt gatagagtct
     2221 atcattctgt tactaaaaat ttgacaactc ggtttgctga cctactggtt actgtatcac
     2281 ttacccgagt taacgagtct agaaacgtgt gaaagaaaca attgaggttt tagagctaga
     2341 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     2401 gctccctagt ctataaatat aagagaccct cttatagtaa gcagagttgt tggagacgtt
     2461 cttgatccgt ttaatagatc aatcactcta aaggttactt atggccaatc gcggccgggt
     2521 acgtgttcga gcgtgcttgg tagtagcgac tccatggttt tagagctaga aatagcaagt
     2581 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctccctagt
     2641 ctataaatat aagagaccct cttatagtaa gcagagttgt tggagacgtt cttgatccgt
     2701 ttaatagatc aatcactcta aaggttactt atggccaatc gcggccgggt acgtgttcga
     2761 gccaggttga ttgtcttctc aagcaggttt tagagctaga aatagcaagt taaaataagg
     2821 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctccctagt ctataaatat
     2881 aagagaccct cttatagtaa gcagagttgt tggagacgtt cttgatccgt ttaatagatc
     2941 aatcactcta aaggttactt atggccaaag tgccgggcat gtcccgaaga cattaaacta
     3001 cggttcttta agtagatccg tgtctgaagt tttaggttca atttaaacct acgagattga
     3061 cattctcgac tgatcttgat tgatcggtaa gtcttttgta atttaatttt ctttttgatt
     3121 ttattttaaa ttgttatctg tttctgtgta tagactgttt gagatcggcg tttggccgac
     3181 tcattgtctt accatagggg aacggacttt gtttgtgttg ttattttatt tgtattttat
     3241 taaaattctc aacgatctga aaaagcctcg cggctaagag attgttgggg ggtgagtaag
     3301 tacttttaaa gtgatgatgg ttacaaaggc aaaaggggta aaacccctcg cctacgtaag
     3361 cgttattacg cccgtctgta cttatatcag tacactgacg agtccctaaa ggacgaaacg
     3421 ggagaacgct agccaccacc accaccacca cgtgtgaatt acaggtgacc agctcgaatt
     3481 tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
     3541 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
     3601 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
     3661 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
     3721 catctatgtt actagatcgg gaattaaact atcagtgttt gacaggatat attggcgggt
     3781 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
     3841 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
     3901 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
     3961 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
     4021 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
     4081 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
     4141 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
     4201 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
     4261 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     4321 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     4381 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     4441 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     4501 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     4561 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     4621 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     4681 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     4741 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     4801 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     4861 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     4921 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     4981 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     5041 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     5101 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     5161 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     5221 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     5281 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     5341 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     5401 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     5461 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     5521 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     5581 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     5641 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     5701 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     5761 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     5821 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     5881 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     5941 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     6001 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     6061 cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa
     6121 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     6181 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     6241 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     6301 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     6361 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     6421 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     6481 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     6541 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     6601 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     6661 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     6721 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     6781 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     6841 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     6901 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     6961 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     7021 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     7081 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     7141 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     7201 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     7261 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     7321 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     7381 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     7441 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     7501 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     7561 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     7621 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     7681 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     7741 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     7801 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     7861 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     7921 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     7981 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8041 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8101 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8161 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8221 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8281 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8341 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8401 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8461 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8521 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8581 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8641 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8701 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8761 gtctgacgct cagtggaacg aaaactcacg ttaagggctg atgaatcccc taatgatttt
     8821 tatcaaaatc attaagttaa ggtagataca catcttgtca tatgatcaaa tggtttcgcc
     8881 aaaaatcaat aatcagacaa caaaatgtgc gaactcgata ttttacacga ctctctttac
     8941 caattctgcc ccgaattaca cttaaaacga ctcaacagct taacgttggc ttgccacgcc
     9001 ttacttgact gtaaaactct cactcttacc gaacttggcc gtaacctgcc aaccaaagcg
     9061 agaacaaaac ataacatcaa acgaatcgac cgattgttag gtaatcgtca cctccacaaa
     9121 gagcgactcg ctgtataccg ttggcatgct agctttatct gttcgggcaa tacgatgccc
     9181 attgtacttg ttgactggtc tgatatccgt gagcaaaaac ggcttatggt attgcgagct
     9241 tcagtcgcac tacacggtcg ttctgttact ctttatgaga aagcgttccc gctttcagag
     9301 caatgttcaa agaaagctca tgaccaattt ctagccgacc ttgcgagcat tctaccgagt
     9361 aacaccacac cgctcattgt cagtgatgct ggctttaaag tgccatggat tctagcaact
     9421 aacttacctg ttgaaattcg aacacccaaa caacttgtta atatctattc gaagcgaatg
     9481 cagattgaag aaaccttccg agacttgaaa agtcctgcct acggactagg cctacgccat
     9541 agccgaacga gcagctcaga gcgttttgat atcatgctgc taatcgccct gatgcttcaa
     9601 ctaacatgtt ggcttgcggg cgttcatgct cagaaacaag gttgggacaa gcacttccag
     9661 gctaacacag tcagaaatcg aaacgtactc tcaacagttc gcttaggcat ggaagttttg
     9721 cggcattctg gctacacaat aacaagggaa gactcactcg tggctgcaac cctgcttact
     9781 caaaatctat tcacacatgg ttacgttttg gggaaattat gaggggatct ctcagcgtta
     9841 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
     9901 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
     9961 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
    10021 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
    10081 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
    10141 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
    10201 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
    10261 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
    10321 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
    10381 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
    10441 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
    10501 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
    10561 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
    10621 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
    10681 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
    10741 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
    10801 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
    10861 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
    10921 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
    10981 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
    11041 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
    11101 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    11161 actgaattaa cgccgaatta attcctaggc caccatgttg ggcccggcgc gcc
//
