LOCUS       sequence-305971-        1460 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Qb coat protein fused to three repeats of the blue fluorescent 
            protein under a Efla promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Katz N, Tripto E, Granik N, Goldberg S, Atar O, Yakhini Z, Orenstein
            Y, Amit R
  TITLE     Overcoming the design, build, test bottleneck for synthesis of 
            nonrepetitive protein-RNA cassettes.
  JOURNAL   Nat Commun. 2021 Mar 11;12(1):1576. doi: 10.1038/s41467-021-21578-6.
  PUBMED    33707432
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-11"; volume: "12(1):1576. doi"; pages: " 
            10.1038/s41467-021-21578-6"
COMMENT     Sequence Label: Ef1a-QCP-3xBFP
FEATURES             Location/Qualifiers
     source          1..1460
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      55..64
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gggactgccg cagaccaggt gtcgtgacgc ggttccggtt aaggtgaacg cgccgccacc
       61 atggcaaaac ttcaggcaat tacgttaagt ggtatcggta agaacggtga tgtcaccttg
      121 aatttgaacc ctcgtggcgt caacccgacg aatggtgtgg ctgcgctgag tgaagcgggc
      181 gcggtgcctg ctttggaaaa acgtgtcacg atctctgtct ctcaaccgag ccgtaatcgt
      241 aaaaactata aagtacaggt caagatccaa aatcctacca gctgcacggc atccggcacg
      301 tgcgatccga gcgtgacgcg ttccgcgtat gcggatgtca cgtttagttt cacgcaatat
      361 agtaccgatg aagaacgcgc actggttcgc acggaactta aagccctttt ggctgatccg
      421 atgctgattg atgcgatcga taacctgaat cctgcctatg ctagcctgca gggatccgga
      481 agtatggtgt ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg
      541 gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac
      601 gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc
      661 gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc
      721 atccccgact tcttcaagca gtccttccct gagggcttca catggggaga gagtcaccac
      781 atacgaagaa cggggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
      841 ctcatctaca acgtcaggat tcagaagggg tgaacttcac atccaacggc cctgtgatgc
      901 agaagaaaac actcgggctg ggaggccttc accgagacgc tgtaccccgc tgacggcggg
      961 cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa
     1021 cgccaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta
     1081 ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgaaacct acgtcgagca
     1141 gcaccaaggt ggcagtggcc agatactgcg acctccctag caaactgggg gcacaagctt
     1201 aatctcgagc caagaaaaag cggaaatggt attctagata atccacctct ggattacaaa
     1261 tttgggaaag aattgacggg gattcctaac aaggttgccc cttttaccca aggggaaacc
     1321 ccgctttaag gccttgtatc aaggcttttg tttcccgagg ggtttttttt tcccccttta
     1381 aaaacagggt tgttcccttt taaaaaagtt ggcccctttt tcacaagggg ggttggggct
     1441 gttttttttt cgcccccccc
//
