LOCUS       sequence-306067-        9858 bp    DNA     circular SYN 15-APR-2025
DEFINITION  loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG 
            assembly vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Nonet ML
  TITLE     Efficient Transgenesis in Caenorhabditis elegans Using Flp 
            Recombinase-Mediated Cassette Exchange.
  JOURNAL   Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 
            10.1534/genetics.120.303388.
  PUBMED    32513816
REFERENCE   2  (bases 1 to 9858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2020 Jun 8. pii: genetics.120.303388. doi: 
            10.1534/genetics.120.303388."
COMMENT     Sequence Label: pNM3749
FEATURES             Location/Qualifiers
     source          1..9858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      36..623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     524..543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(635..662)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(686..1501)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1409..1428
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1502..1604)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(1605..1636)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    complement(1719..1752)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1762..1791
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3608..3931)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5849..5873
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5876..6901
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5876..6901
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6902..6926)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7279..7312)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7938..7971
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(8018..8046)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     8794..8814
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(9837..9854)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9837..9853)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgggcctttc ttcggtagaa aatcaaagga tcttcttgag atcctttttt tctgcgcgta
       61 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      121 gagctaccaa ctctttttcc gaggtaactg gcttcagcag agcgcagata ccaaatactg
      181 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
      241 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      301 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      361 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      421 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      481 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      541 tttatagtcc tgtcgggttt cgccacctct gacttgagca tcgatttttg tgatgctcgt
      601 caggggggcg gagcctatgg aaaaacgcca gcaacgcaga aaggcccacc cgaaggtgag
      661 ccaggtgatt acatttaggt cctcattaga aaaactcatc gagcatcaag tgaaactgca
      721 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
      781 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
      841 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
      901 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaagagt ttatgcattt
      961 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcaccaa
     1021 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgccgttaa
     1081 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     1141 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttccctggga
     1201 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     1261 gaggcataaa ttccgtcagc cagtttagcc tgaccatctc atctgtaaca tcattggcaa
     1321 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat
     1381 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     1441 catccatgtt ggaatttaat cgcggcttca agcaagacgt ttcccgttga atatggctca
     1501 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     1561 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaagtcaa aagcctccgg
     1621 tcggaggctt ttgactttct gctatggagg tcaggtatga tttttgaatt cgctcttcgg
     1681 cgacgtacga actaggctgt ttcgaaggta agtttaaaat aacttcgtat agcatacatt
     1741 atacgaagtt attttcaggg agagcaaaag cttatctccg aggaggacct ctaaggatga
     1801 tcgacgccaa cgtcgttgaa ttttcaaatt ttaaatactg aatatttgtt ttttttccta
     1861 ttatttattt attctctttg tgtttttttt cttgctttct aaaaaattaa ttcaatccaa
     1921 atctaaacat ttttttttct ctttccgtct cccaattcgt attccgctcc tctcatctga
     1981 acacaatgtg caagtttatt tatcttctcg ctttcatttc attaggacgt ggggggaatt
     2041 ggtggaaggg ggaaacacac aaaaggatga tggaaatgaa ataaggacac acaatatgca
     2101 acaacattca attcagaaat atggaggaag gtttaaaaga aaacataaaa atatatagag
     2161 gaggaaggaa actagctgcc ctcgattgtc tatcttccat tctcaccaag tttcaacaac
     2221 tttattcagt cattcttctc tggttcattt cccaaccaat cttcccgaat aaagcccatc
     2281 tgtcctttcg gccacattcc tcacctacat ccgtactcct tatctcccgt tcttatccag
     2341 tcttttatcg tctctgcgtc tcgcacactg tctcgtcggt aatgtaaaaa aaaaacaaaa
     2401 aaaacttgaa aacttgcgga aagatttcct aataaaagga gacgaatccg gtaggaaatc
     2461 gacaagtctc tgcttctcga agactaggtg agttacattt ctttggccga attattttga
     2521 aatcctaaat ttgataattt cagagatgtc tgtaaaactt gcgtgttatg taacggcttc
     2581 ggtcactgtc gccactctta tggtatgctt catgaccatg tcaaccatct actcggaggt
     2641 tgatggattc agagagaagc ttgataccga gatgaatgtg ttcagagtga gttaaattat
     2701 attttgaatt ttaaataatt tttaattttc tagcaatcta ccaatggatt gtggaaggac
     2761 atagttgtca tcggaagatc tagcaagcgt gtccgttgtc aatatgaaga gaccaacgct
     2821 accccaactc cacatgctga tggatcccca tctgctccac caggtcaacc accagcagtt
     2881 ccaccagtct tcaaccagcc aaagactcca aatggagcca atggaaatgg accaacctgc
     2941 aactgcaatg ctgataacaa gtgcccagct ggaccatccg gaccaaaggg agttccagga
     3001 gttccaggac tcgacggagt tccaggactt gacggtgttc caggagttgg agctgatgat
     3061 atcgctccac aacgcgagtc tgtcggatgc ttcacttgcc cacaaggacc agttggacca
     3121 ccaggagctc ttggaagacc aggaccacgt ggacttccag gaccaagagg acaaaatgga
     3181 aacccaggaa gagatggaca accaggacat ccaggagagc aaggatcatc cggtcaaatc
     3241 ggaaagatcg gagagccagg accaccagga gagaagggac gcgacgccga gcatccaatc
     3301 ggaagaccag gaccaaaggg accaagagga gatcaaggac caacaggacc agctggacag
     3361 aacggtcttc acggaccacc aggagagcca ggaaccgttg gaccagaagg accatctgga
     3421 aagcaaggac gtcaaggacc agacggaacc cagggagaga ctggaccaga cggaagacca
     3481 ggaaaggatg ccgagtactg ccagtgccca gacaagtctc caccatcaga ggctgtcaac
     3541 gccaaccgtg gatacagaaa tatctaaatt gttggtgttt tctaataaaa tatttgagat
     3601 tcagtgagac ttttttcttg gcggcacaat aaagtttcct ctttttgttg ggtcactcaa
     3661 cgaacacaat acaaatattt gagcgctcgc atttttctca acctttcggg tttaaagcac
     3721 ttcaaaatgg aattgaagag agattgtgaa agtacatcag acagaatcac attttgatag
     3781 cattcacttc actcagatgc aaacttttca gtgactataa aaagtcataa aataataaca
     3841 ttaaaaaagc aaataaatta gcgagagaat tttttgacaa aaagaaagaa gagtgataga
     3901 gaagaaggga atgcttgaaa ggattttgca tttatcttaa tcaccgtctt cgagaagacg
     3961 aaccatggcg ccagtttcag aatccaagtt tctaatatag ttcatcacaa tgttgacgtt
     4021 ggtccaacct ccggcttgca taatctctgg gatggaaact ccagcgcgag ccatgtcacg
     4081 ggcagctccg acacgggcgg aatgtccaga ccaagccaag taacgttgtc cggaatcatc
     4141 cttggctccg tagatgagac ggtgggtagc ttcgaagatt cctgaaaatt tattttcagc
     4201 ttattcttag ctaatatgtt gtttaaactt accttcaaga gctcgagtcg agagttgtga
     4261 tgttgcactc ggggcagcaa ctccgttctt gcgaacacga cagaacaggt agttgtttgg
     4321 atcatcggcg actccggaaa ccgaaatcca gcgctcaacg agcttggtaa ctccaaggga
     4381 gagcgctttt tcgactccgg cagtcgagac cagcgttttt gtacgtccga tgtggatgag
     4441 cattcttcct ccgtctgtgc gactaatatc cttgacgcga atgcgggcaa tttcggcgat
     4501 acggagaagt gtgttgtaag caattccgag gaaggcgagg tttcggatgt cctggcaacg
     4561 atcggaattt tccataagag aacggacctg atcgaagtcg gttctgaaaa tttcgtcaag
     4621 tcttaggatt gaatacatta atatttaaac ttacctttcg aaggcgaggg cttgcttggc
     4681 tctctctcca gcatcaacat tttccttgcg gatacgtctc attacgagag atacggcatt
     4741 agaatctgat gggcgtggaa gtccagatcg gcgatggagc atattgagct gtccgagatg
     4801 ttgttggatc gtcttcaccg ccaatccacg agcttggagg tagagcaagt agtcacgaac
     4861 gtcttctggc tcggctggga accacttgcg gttgttaagc ttgcaccagg cagcccaaga
     4921 acggcacact gagagaagca tcttccaggt gtgctcggag aaggcttgtc tgtcacggaa
     4981 catatccatg aggttcttgc gaacttcatc acttgtcgcg tcaactggaa gtgctggaag
     5041 attttgatga acagtgagaa gatttgacat attttcgaag ttttttagat gcactagaac
     5101 aaagcgtgtt ggcttcctct gagcccgctt tccttatata cccgcattct gcagccttac
     5161 agaatgttct agaaggtcct agatgcattc gtttgaaaat actcccggtg ggtgcaaaga
     5221 gacgcagacg gaaaatgtat ctgggtctct ttattgtgta cactactttt ccatgtaccg
     5281 aatgtgagtc gccctccttt tgcaacaagc agctcgaatg ttctagaaaa aggtggaaaa
     5341 tagtataaat accgttgaaa ataaataccg aacaacattt gctctaattg tgaaattaga
     5401 aatcttcaaa ctataatcat gtcactttac cactatttcc gtccagctca acgttccgtt
     5461 tttggtgatc atttttgctt tcgtcgtaaa tctacacacg cgtctcttcc gtgcgagagt
     5521 ccaagccagc agccaaattc gttgactgag tattcaacgt ttatacgttg tcggcaacga
     5581 gaaataggaa aatgcatcgg gaaatgttct tttttcgatt ttttccaagg ttttgacaaa
     5641 ttttaccacg aattttgcta tgttttcaat taaaaaatat gttattcaac tgtttctatg
     5701 aggaaaataa ggctttgcat gtaattttct tattcagcat aatttttaat taatttgaat
     5761 tttctgtcct aacgtttatt ttgttttctt ggttatgact gatctgaaat taatttttga
     5821 attttaaggt aatatgtcag gcggtgccgc aagtttgtac aaaaaagcag gctccatgaa
     5881 aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt
     5941 ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg
     6001 agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta
     6061 tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga
     6121 attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga
     6181 cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca tggatgcgat
     6241 cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg
     6301 tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg
     6361 gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat
     6421 gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa
     6481 caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt
     6541 cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat
     6601 ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct
     6661 ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa
     6721 tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg
     6781 gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt
     6841 agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaata
     6901 gacccagctt tcttgtacaa agtgggtcca attactcttc aacatcccta catgctcttt
     6961 ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt aatttctttg
     7021 ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca aaaatagaat
     7081 taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat aataattcta
     7141 tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc tgataaattt
     7201 ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt
     7261 ttatgaccgc aatttttaat aacttcgtat agcatacatt atacgaagtt attttcaggg
     7321 tgggagaaaa tgcggagaga agaacccagc atcttaataa aattatataa ttttgaataa
     7381 aatcaaatta aaaataagat taattgaaac acaacaatag aatgcagaaa attactcata
     7441 agaaataatt tattttgaaa gaagttgtgt cgatttacga taaaaactac acgcaaacga
     7501 ggctccgcgt ttacaccgtt tggcaatgga gcgcgcttgc tattagattt taggccagct
     7561 caacgaaagt ttaatttatt tttcttcttt ttttccattt ttttgagtaa aatatcgaat
     7621 tttttgtatt aattctgtaa attttataat ttgcaactat tttggcgtat tctgcttgtt
     7681 tttatcagcg aatttttatg catttcagtc aaaagtttga gcaggaaaaa tgacatttag
     7741 gactttgtaa aattgtattc ttaaatttgc ttgaaaacct acgtgtacaa ttgattttat
     7801 tgaatttttc ttacgtttcc cgttgtatct tattgtattt aactttcatc ataaaataat
     7861 ttatttccag gtaaaatatc cgacgctctc gtggatcccc gggattggct cagaaaaaaa
     7921 ggtacgagca ggcttacgaa gttcctattc tctagaaagt ataggaactt cggtaccggt
     7981 agaaaaaatg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     8041 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     8101 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatgggt
     8161 aagtttaaac atatatatac taactaaccc tgattattta aattttcagc caacacttgt
     8221 cactactttc tgttatggtg ttcaatgctt ctcgagatac ccagatcata tgaaacggca
     8281 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
     8341 agatgacggg aactacaaga cacgtaagtt taaacagttc ggtactaact aaccatacat
     8401 atttaaattt tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
     8461 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
     8521 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttgt
     8581 aagtttaaac atgattttac taactaacta atctgattta aattttcaga acttcaaaat
     8641 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
     8701 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
     8761 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
     8821 tgggattaca catggcatgg atgaactata caaatgcccg ggggatcggt ggagctccac
     8881 cggtggcggc cgctctagaa ctagtccacc aaagccatct gccaagggag ccaagaaggc
     8941 cgccaagacc gtcgttgcca agccaaagga cggaaagaag agacgtcatg cccgcaagga
     9001 atcgtactcc gtctacatct accgtgttct caagcaagtt cacccagaca ccggagtctc
     9061 ctccaaggcc atgtctatca tgaactcctt cgtcaacgat gtattcgaac gcatcgcttc
     9121 ggaagcttcc cgtcttgctc attacaacaa acgctcaacg atctcatccc gcgaaattca
     9181 aaccgctgtc cgtttgattc tcccaggaga acttgccaag cacgccgtgt ctgagggaac
     9241 caaggccgtc accaagtaca cttccagcaa gtaaactagt ggatctgcaa cgcgtggagc
     9301 tagcaggctg aaaactaccc caccgaaccc aacggccctc tttagggcca caaatcttat
     9361 aatcctatgt tattaaccaa gtgaatgaat aattttcaat tatactcttt cataattttc
     9421 aggttttgca ttttccaagt tcagttaatt tgagattaac ccagtattca ttttctaaaa
     9481 gtcattcttt cagagagaga aattcaaagc atcttcggaa ttctatcttt caaattgtag
     9541 atgggattct tcgaatattt ccaaaaactt gtatttccgt agtttatgaa gttgcacaga
     9601 gaaagggaag ttcctatact atttgaagaa taggaacttc atgcctgcag gacgcgaaga
     9661 gcaagcttgg atcgacgaga gcagcgcgac tggatctgtc gcccgtctca aacgcaaccc
     9721 tccggcggtc gcatatcatt caggacgagc ctcagactcc agcgtaactg gactgcaatc
     9781 aactcactgg ctcaccttcc ggtccacgat cagctagaat caagctgact agataaactg
     9841 gccgtcgttt tacacggg
//
