LOCUS       sequence-306077-        9451 bp    DNA     circular SYN 14-APR-2025
DEFINITION  tetO 7X enhancer with ∆mec-7 basal promoter driving GFP-C1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Nonet ML
  TITLE     Efficient Transgenesis in Caenorhabditis elegans Using Flp 
            Recombinase-Mediated Cassette Exchange.
  JOURNAL   Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 
            10.1534/genetics.120.303388.
  PUBMED    32513816
REFERENCE   2  (bases 1 to 9451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2020 Jun 8. pii: genetics.120.303388. doi: 
            10.1534/genetics.120.303388."
COMMENT     Sequence Label: pNM3774
FEATURES             Location/Qualifiers
     source          1..9451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..34
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             35..1060
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             35..1060
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     terminator      complement(2877..3200)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(4793..4812)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4794..4812)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4822..4839)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4822..4838)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4831..4853)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4980..5435
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5067..5086)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5277..5298
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5461..5565
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5566..5634,5635..6426)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5784..5803)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6597..7185
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7086..7105
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7339..7356
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7472..7493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7508..7513,7514..7531,7532..7538)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7546..7562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7551..7573
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7570..7586
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7570..7586
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7605..7625
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7607..7625
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(7671..7704)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7784..7802
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7820..7838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7856..7874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7892..7910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7928..7946
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7964..7982
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8000..8018
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      9063..9386
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
ORIGIN
        1 gaagttccta ttctctagaa agtataggaa cttcatgaaa aagcctgaac tcaccgcgac
       61 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
      121 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
      181 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
      241 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
      301 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
      361 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
      421 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
      481 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
      541 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
      601 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
      661 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
      721 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
      781 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
      841 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
      901 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
      961 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     1021 aaaccgacgc cccagcactc gtccgagggc aaaggaatag gcgggatgat cgacgccaac
     1081 gtcgttgaat tttcaaattt taaatactga atatttgttt tttttcctat tatttattta
     1141 ttctctttgt gttttttttc ttgctttcta aaaaattaat tcaatccaaa tctaaacatt
     1201 tttttttctc tttccgtctc ccaattcgta ttccgctcct ctcatctgaa cacaatgtgc
     1261 aagtttattt atcttctcgc tttcatttca ttaggacgtg gggggaattg gtggaagggg
     1321 gaaacacaca aaaggatgat ggaaatgaaa taaggacaca caatatgcaa caacattcaa
     1381 ttcagaaata tggaggaagg tttaaaagaa aacataaaaa tatatagagg aggaaggaaa
     1441 ctagctgccc tcgattgtct atcttccatt ctcaccaagt ttcaacaact ttattcagtc
     1501 attcttctct ggttcatttc ccaaccaatc ttcccgaata aagcccatct gtcctttcgg
     1561 ccacattcct cacctacatc cgtactcctt atctcccgtt cttatccagt cttttatcgt
     1621 ctctgcgtct cgcacactgt ctcgtcggta atgtaaaaaa aaaacaaaaa aaacttgaaa
     1681 acttgcggaa agatttccta ataaaaggag acgaatccgg taggaaatcg acaagtctct
     1741 gcttctcgaa gactaggtga gttacatttc tttggccgaa ttattttgaa atcctaaatt
     1801 tgataatttc agagatgtct gtaaaacttg cgtgttatgt gacggcttcg gtcactgtcg
     1861 ccactcttat ggtatgcttc atgaccatgt caaccatcta ctcggaggtt gatggattca
     1921 gagagaagct tgataccgag atgaatgtgt tcagagtgag ttaaattata ttttgaattt
     1981 taaataattt ttaattttct agcaatctac caatggattg tggaaggaca tagttgtcat
     2041 cggaagatct agcaagcgtg tccgttgtca atatgaagag accaacgcta ccccaactcc
     2101 acatgctgat ggatccccat ctgctccacc aggtcaacca ccagcagttc caccagtctt
     2161 caaccagcca aagactccaa atggagccaa tggaaatgga ccaacctgca actgcaatgc
     2221 tgataacaag tgcccagctg gaccatccgg accaaaggga gttccaggag ttccaggact
     2281 cgacggagtt ccaggacttg acggtgttcc aggagttgga gctgatgata tcgctccaca
     2341 acgcgagtct gtcggatgct tcacttgccc acaaggacca gttggaccac caggagctct
     2401 tggaagacca ggaccacgtg gacttccagg accaagagga caaaatggaa acccaggaag
     2461 agatggacaa ccaggacatc caggagagca aggatcatcc ggtcaaatcg gaaagatcgg
     2521 agagccagga ccaccaggag agaagggacg cgacgccgag catccaatcg gaagaccagg
     2581 accaaaggga ccaagaggag atcaaggacc aacaggacca gctggacaga acggtcttca
     2641 cggaccacca ggagagccag gaaccgttgg accagaagga ccatctggaa agcaaggacg
     2701 tcaaggacca gacggaaccc agggagagac tggaccagac ggaagaccag gaaaggatgc
     2761 cgagtactgc cagtgcccag acaagtctcc accatcagag gctgtcaacg ccaaccgtgg
     2821 atacagaaat atctaaattg ttggtgtttt ctaataaaat atttgagatt cagtgagact
     2881 tttttcttgg cggcacaata aagtttcctc tttttgttgg gtcactcaac gaacacaata
     2941 caaatatttg agcgctcgca tttttctcaa cctttcgggt ttaaagcact tcaaaatgga
     3001 attgaagaga gattgtgaaa gtacatcaga cagaatcaca ttttgatagc attcacttca
     3061 ctcagatgca aacttttcag tgactataaa aagtcataaa ataataacat taaaaaagca
     3121 aataaattag cgagagaatt ttttgacaaa aagaaagaag agtgatagag aagaagggaa
     3181 tgcttgaaag gattttgcat ttatcttaat caccgtcttc gagaagacga accatggcgc
     3241 cagtttcaga atccaagttt ctaatatagt tcatcacaat gttgacgttg gtccaacctc
     3301 cggcttgcat aatctctggg atggaaactc cagcgcgagc catgtcacgg gcagctccga
     3361 cacgggcgga atgtccagac caagccaagt aacgttgtcc ggaatcatcc ttggctccgt
     3421 agatgagacg gtgggtagct tcgaagattc ctgaaaattt attttcagct tattcttagc
     3481 taatatgttg tttaaactta ccttcaagag ctcgagtcga gagttgtgat gttgcactcg
     3541 gggcagcaac tccgttcttg cgaacacgac agaacaggta gttgtttgga tcatcggcga
     3601 ctccggaaac cgaaatccag cgctcaacga gcttggtaac tccaagggag agcgcttttt
     3661 cgactccggc agtcgagacc agcgtttttg tacgtccgat gtggatgagc attcttcctc
     3721 cgtctgtgcg actaatatcc ttgacgcgaa tgcgggcaat ttcggcgata cggagaagtg
     3781 tgttgtaagc aattccgagg aaggcgaggt ttcggatgtc ctggcaacga tcggaatttt
     3841 ccataagaga acggacctga tcgaagtcgg ttctgaaaat ttcgtcaagt cttaggattg
     3901 aatacattaa tatttaaact tacctttcga aggcgagggc ttgcttggct ctctctccag
     3961 catcaacatt ttccttgcgg atacgtctca ttacgagaga tacggcatta gaatctgatg
     4021 ggcgtggaag tccagatcgg cgatggagca tattgagctg tccgagatgt tgttggatcg
     4081 tcttcaccgc caatccacga gcttggaggt agagcaagta gtcacgaacg tcttctggct
     4141 cggctgggaa ccacttgcgg ttgttaagct tgcaccaggc agcccaagaa cggcacactg
     4201 agagaagcat cttccaggtg tgctcggaga aggcttgtct gtcacggaac atatccatga
     4261 ggttcttgcg aacttcatca cttgtcgcgt caactggaag tgctggaaga ttttgatgaa
     4321 cagtgagaag atttgacata ttttcgaagt tttttagatg cactagaaca aagcgtgttg
     4381 gcttcctctg agcccgcttt ccttatatac ccgcattctg cagccttaca gaatgttcta
     4441 gaaggtccta gatgcattcg tttgaaaata ctcccggtgg gtgcaaagag acgcagacgg
     4501 aaaatgtatc tgggtctctt tattgtgtac actacttttc catgtaccga atgtgagtcg
     4561 ccctcctttt gcaacaagca gctcgaatgt tctagaaaaa ggtggaaaat agtataaata
     4621 ccgttgaaaa taaataccga acaacatttg ctctaattgt gaaattagaa atcttcaaac
     4681 tataatcatg tcactttacc actatttccg tccagctcaa cgttccgttt ttggtgatcg
     4741 taagcttatc gataccgtcg acccgttaac cgggggcccg gtacccaatt cgccctatag
     4801 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     4861 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     4921 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     4981 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5041 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5101 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     5161 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     5221 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     5281 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     5341 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5401 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     5461 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5521 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5581 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5641 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5701 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5761 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5821 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5881 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5941 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6001 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6061 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6121 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6181 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6241 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6301 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6361 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6421 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6481 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6541 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6601 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6661 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6721 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6781 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6841 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6901 cagcggtcgg gctgaatggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6961 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7021 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7081 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7141 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7201 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7261 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7321 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaattcgc ccaatacgca
     7381 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     7441 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     7501 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     7561 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     7621 aaagggaaca aaagctggag ctccaccgcg gtggcggccg ctctagatgg ataacttcgt
     7681 atagcataca ttatacgaag ttatacgggc gaattggagc tccaccgcgg tggcggccgc
     7741 tctagaacta gtggcttgca tgcccgtctt cactcgagtt tactccctat cagtgataga
     7801 gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct
     7861 atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc
     7921 agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga
     7981 gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgc
     8041 gagatgtgac tcctcccatt attcgtcttg agtcgcatct tttgtcttgg tccgttgtca
     8101 gtcgagcctc aaggcagccg ttccgccagt gatcgcctgc taagaagttg taggtgtaga
     8161 ggaagaaacg tcgggtccaa atttcaagca acatgagtaa aggagaagaa ttgttcactg
     8221 gagttgtccc aatcctcgtc gagctcgacg gagacgtcaa cggacacaag ttctccgtct
     8281 ccggagaggg agagggagac gccacctacg gaaagctcac cctcaagttc atctgcacca
     8341 ccggaaagct cccagtccca tggccaaccc tcgtcaccac cttctgctac ggagtccaat
     8401 gcttctcccg ttacccagac cacatgaagc gtcacgactt cttcaagtcc gccatgccag
     8461 agggatacgt ccaagagcgt accatcttct tcaaggtaag tttaaacata tatatactaa
     8521 ctactgatta tttaaatttt caggacgacg gaaactacaa gacccgtgcc gaggtcaagt
     8581 tcgagggaga caccctcgtc aaccgtatcg agctcaaggt aagtttaaac agttcggtac
     8641 taactaacca tacatattta aattttcagg gaatcgactt caaggaggac ggaaacatcc
     8701 tcggacacaa gctcgagtac aactacaact cccacaacgt ctacatcatg gccgacaagc
     8761 aaaagaacgg aatcaaggtc aacttcaagg taagtttaaa catgatttta ctaactaact
     8821 aatctgattt aaattttcag atccgtcaca acatcgagga cggatccgtc caactcgccg
     8881 accactacca acaaaacacc ccaatcggag acggaccagt cctcctccca gacaaccact
     8941 acctctccac ccaatccgcc ctctccaagg acccaaacga gaagcgtgac cacatggtcc
     9001 tcctcgagtt cgtcaccgcc gccggaatca cccacggaat ggacgagctc tacaagtaat
     9061 aaatgcaaaa tcctttcaag cattcccttc ttctctatca ctcttctttc tttttgtcaa
     9121 aaaattctct cgctaattta tttgcttttt taatgttatt attttatgac tttttatagt
     9181 cactgaaaag tttgcatctg agtgaagtga atgctatcaa aatgtgattc tgtctgatgt
     9241 actttcacaa tctctcttca attccatttt gaagtgcttt aaacccgaaa ggttgagaaa
     9301 aatgcgagcg ctcaaatatt tgtattgtgt tcgttgagtg acccaacaaa aagaggaaac
     9361 tttattgtgc cgccaagaaa aaagtctcag tacccgggaa gttcctatac tatttgaaga
     9421 ataggaactt ctgcaggaat tcgatatcgg t
//
