LOCUS       sequence-306110-        8816 bp    DNA     circular SYN 15-APR-2025
DEFINITION  To knock down MTH1 (Mut T Homolog 1) after addition of doxycline.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Patel A, Burton DG, Halvorsen K, Balkan W, Reiner T, Perez-Stable C,
            Cohen A, Munoz A, Giribaldi MG, Singh S, Robbins DJ, Nguyen DM, Rai 
            P
  TITLE     MutT Homolog 1 (MTH1) maintains multiple KRAS-driven pro-malignant 
            pathways.
  JOURNAL   Oncogene. 2015 May 14;34(20):2586-96. doi: 10.1038/onc.2014.195. 
            Epub 2014 Jul 14.
  PUBMED    25023700
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2014.195"; journalName: "Oncogene"; date: "2015-05-14- 
            14"; volume: "34"; issue: "20"; pages: "2586-96"
COMMENT     Sequence Label: plko-tet-on puromycin-shMTH1
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2005..2099
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2016..2035
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2053..2071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2081..2099
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2200..2317
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2366..2876
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2795..2813
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          2885..3457
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3465..3484
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3512..3530
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3541..4161
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3614..3633)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    4196..4769
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4363..4380)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4590..4609
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4789..5388
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4789..4808)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5285..5305
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5516..5749
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5821..5942
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5858..5877)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5912..5931
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5982..6117
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6138..6156)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6166..6183)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6166..6182)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6175..6197)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6324..6779
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6411..6430)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6621..6642
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6805..6909
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6910..6978,6979..7770)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7128..7147)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7941..8529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8430..8449
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8683..8700
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca
     1981 cgagactagc ctcgagcggc cgcaatattt gcatgtcgct atgtgttctg ggaaatcacc
     2041 ataaacgtga aatccctatc agtgatagag acttataagt tccctatcag tgatagagac
     2101 accgggaaat tccacgggta cttcaactcg agttgaagta cccgtggaat ttctttttga
     2161 attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2221 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2281 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2341 agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa ggcagccctg
     2401 ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc
     2461 ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc
     2521 ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt
     2581 tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg
     2641 gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct
     2701 gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg
     2761 gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg
     2821 agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg
     2881 atctgtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt
     2941 atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca
     3001 ccatggaccc tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc
     3061 tcctcttatt ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta
     3121 acgaattttt aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact
     3181 tttttttcaa ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat
     3241 tgttataatt aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa
     3301 tattcttatt ggtagaaaca actacatcct ggtcatcatc ctgcctttct ctttatggtt
     3361 acaatgatat acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc
     3421 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat
     3481 tgtgctgtct catcattttg gcaaagaatt gtaatacgac tcactatagg gcgaattgat
     3541 atgtctagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc
     3601 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca
     3661 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta
     3721 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt
     3781 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat
     3841 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta
     3901 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt
     3961 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca
     4021 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa
     4081 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa
     4141 cttaaatgtg aaagtgggtc cgcgtacagc ggctcccggg agttctaggg atctgcccct
     4201 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     4261 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     4321 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     4381 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     4441 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     4501 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     4561 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     4621 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     4681 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     4741 tggttttcct ttgaaaaaca cgatgataag gatccaccgg agcttaccat gaccgagtac
     4801 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     4861 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     4921 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     4981 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     5041 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     5101 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     5161 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     5221 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     5281 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     5341 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac
     5401 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca
     5461 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     5521 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     5581 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     5641 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5701 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     5761 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     5821 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5881 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5941 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     6001 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6061 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6121 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     6181 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     6241 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     6301 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6361 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6421 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6481 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6541 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     6601 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     6661 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     6721 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     6781 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6841 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     6901 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6961 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7021 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7081 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7141 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7201 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7261 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7321 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7381 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7441 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7501 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7561 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7621 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7681 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7741 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     7801 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7861 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7921 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7981 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8041 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8101 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8161 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8221 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8281 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8341 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8401 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8461 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8521 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8581 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8641 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8701 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     8761 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaat
//
