LOCUS       sequence-306216-        9085 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression fusion protein FTO-dCas9 in Mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Liu XM, Zhou J, Mao Y, Ji Q, Qian SB
  TITLE     Programmable RNA N(6)-methyladenosine editing by CRISPR-Cas9 
            conjugates.
  JOURNAL   Nat Chem Biol. 2019 Sep;15(9):865-871. doi: 
            10.1038/s41589-019-0327-1. Epub 2019 Aug 5.
  PUBMED    31383972
REFERENCE   2  (bases 1 to 9085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0327-1"; journalName: "Nat Chem Biol"; date: 
            "2019-09"; volume: "15"; issue: "9"; pages: "865-871"
COMMENT     Sequence Label: FTO-dCas9
FEATURES             Location/Qualifiers
     source          1..9085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     246..265
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        246..264
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1841..5239,5240..5242,5243..5839,5840,5841..5843,
                     5844..5845,5846..5848,5849..5941)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1841..1864,1865..1867,1868..4354,4355..4357,
                     4358..5941)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5942..5962
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5969..5986
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6009..6026)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6015..6239
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6310..6326)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6310..6326)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6323..6345)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6334..6350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6358..6364,6365..6382,6383..6388))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6403..6424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6712..7300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6792..6811)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7471..8262,8263..8331))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8094..8113
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8332..8436)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8515..8534)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8706..9085
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gagatccgcg
      241 gccgctaata cgactcacta tagggagagc cgccaccatg aagcgcaccc cgactgccga
      301 ggaacgagag cgcgaagcta agaaactgag gcttcttgaa gagcttgaag acacttggct
      361 cccttatctg acccccaaag atgatgaatt ctatcagcag tggcagctga aatatcctaa
      421 actaattctc cgagaagcca gcagtgtatc tgaggagctc cataaagagg ttcaagaagc
      481 ctttctcaca ctgcacaagc atggctgctt atttcgggac ctggttagga tccaaggcaa
      541 agatctgctc actccggtat ctcgcatcct cattggtaat ccaggctgca cctacaagta
      601 cctgaacacc aggctcttta cggtcccctg gccagtgaaa gggtctaata taaaacacac
      661 cgaggctgaa atagccgctg cttgtgagac cttcctcaag ctcaatgact acctgcagat
      721 agaaaccatc caggctttgg aagaacttgc tgccaaagag aaggctaatg aggatgctgt
      781 gccattgtgt atgtctgcag atttccccag ggttgggatg ggttcatcct acaacggaca
      841 agatgaagtg gacattaaga gcagagcagc atacaacgta actttgctga atttcatgga
      901 tcctcagaaa atgccatacc tgaaagagga accttatttt ggcatgggga aaatggcagt
      961 gagctggcat catgatgaaa atctggtgga caggtcagcg gtggcagtgt acagttatag
     1021 ctgtgaaggc cctgaagagg aaagtgagga tgactctcat ctcgaaggca gggatcctga
     1081 tatttggcat gttggtttta agatctcatg ggacatagag acacctggtt tggcgatacc
     1141 ccttcaccaa ggagactgct atttcatgct tgatgatctc aatgccaccc accaacactg
     1201 tgttttggcc ggttcacaac ctcggtttag ttccacccac cgagtggcag agtgctcaac
     1261 aggaaccttg gattatattt tacaacgctg tcagttggct ctgcagaatg tctgtgacga
     1321 tgtggacaat gatgatgtct ctttgaaatc ctttgagcct gcagttttga aacaaggaga
     1381 agaaattcat aatgaggtcg agtttgagtg gctgaggcag ttttggtttc aaggcaatcg
     1441 atacagaaag tgcactgact ggtggtgtca acccatggct caactggaag cactgtggaa
     1501 gaagatggag ggtgtgacaa atgctgtgct tcatgaagtt aaaagagagg ggctccccgt
     1561 ggaacaaagg aatgaaatct tgactgccat ccttgcctcg ctcactgcac gccagaacct
     1621 gaggagagaa tggcatgcca ggtgccagtc acgaattgcc cgaacattac ctgctgatca
     1681 gaagccagaa tgtcggccat actgggaaaa ggatgatgct tcgatgcctc tgccgtttga
     1741 cctcacagac atcgtttcag aactcagagg tcagcttctg gaagcaaaac ccagcggcag
     1801 cgagactccc gggacctcag agtccgccac acccgaaagt gataaaaagt attctattgg
     1861 tttagccatc ggcactaatt ccgttggatg ggctgtcata accgatgaat acaaagtacc
     1921 ttcaaagaaa tttaaggtgt tggggaacac agaccgtcat tcgattaaaa agaatcttat
     1981 cggtgccctc ctattcgata gtggcgaaac ggcagaggcg actcgcctga aacgaaccgc
     2041 tcggagaagg tatacacgtc gcaagaaccg aatatgttac ttacaagaaa tttttagcaa
     2101 tgagatggcc aaagttgacg attctttctt tcaccgtttg gaagagtcct tccttgtcga
     2161 agaggacaag aaacatgaac ggcaccccat ctttggaaac atagtagatg aggtggcata
     2221 tcatgaaaag tacccaacga tttatcacct cagaaaaaag ctagttgact caactgataa
     2281 agcggacctg aggttaatct acttggctct tgcccatatg ataaagttcc gtgggcactt
     2341 tctcattgag ggtgatctaa atccggacaa ctcggatgtc gacaaactgt tcatccagtt
     2401 agtacaaacc tataatcagt tgtttgaaga gaaccctata aatgcaagtg gcgtggatgc
     2461 gaaggctatt cttagcgccc gcctctctaa atcccgacgg ctagaaaacc tgatcgcaca
     2521 attacccgga gagaagaaaa atgggttgtt cggtaacctt atagcgctct cactaggcct
     2581 gacaccaaat tttaagtcga acttcgactt agctgaagat gccaaattgc agcttagtaa
     2641 ggacacgtac gatgacgatc tcgacaatct actggcacaa attggagatc agtatgcgga
     2701 cttatttttg gctgccaaaa accttagcga tgcaatcctc ctatctgaca tactgagagt
     2761 taatactgag attaccaagg cgccgttatc cgcttcaatg atcaaaaggt acgatgaaca
     2821 tcaccaagac ttgacacttc tcaaggccct agtccgtcag caactgcctg agaaatataa
     2881 ggaaatattc tttgatcagt cgaaaaacgg gtacgcaggt tatattgacg gcggagcgag
     2941 tcaagaggaa ttctacaagt ttatcaaacc catattagag aagatggatg ggacggaaga
     3001 gttgcttgta aaactcaatc gcgaagatct actgcgaaag cagcggactt tcgacaacgg
     3061 tagcattcca catcaaatcc acttaggcga attgcatgct atacttagaa ggcaggagga
     3121 tttttatccg ttcctcaaag acaatcgtga aaagattgag aaaatcctaa cctttcgcat
     3181 accttactat gtgggacccc tggcccgagg gaactctcgg ttcgcatgga tgacaagaaa
     3241 gtccgaagaa acgattactc catggaattt tgaggaagtt gtcgataaag gtgcgtcagc
     3301 tcaatcgttc atcgagagga tgaccaactt tgacaagaat ttaccgaacg aaaaagtatt
     3361 gcctaagcac agtttacttt acgagtattt cacagtgtac aatgaactca cgaaagttaa
     3421 gtatgtcact gagggcatgc gtaaacccgc ctttctaagc ggagaacaga agaaagcaat
     3481 agtagatctg ttattcaaga ccaaccgcaa agtgacagtt aagcaattga aagaggacta
     3541 ctttaagaaa attgaatgct tcgattctgt cgagatctcc ggggtagaag atcgatttaa
     3601 tgcgtcactt ggtacgtatc atgacctcct aaagataatt aaagataagg acttcctgga
     3661 taacgaagag aatgaagata tcttagaaga tatagtgttg actcttaccc tctttgaaga
     3721 tcgggaaatg attgaggaaa gactaaaaac atacgctcac ctgttcgacg ataaggttat
     3781 gaaacagtta aagaggcgtc gctatacggg ctggggacga ttgtcgcgga aacttatcaa
     3841 cgggataaga gacaagcaaa gtggtaaaac tattctcgat tttctaaaga gcgacggctt
     3901 cgccaatagg aactttatgc agctgatcca tgatgactct ttaaccttca aagaggatat
     3961 acaaaaggca caggtttccg gacaagggga ctcattgcac gaacatattg cgaatcttgc
     4021 tggttcgcca gccatcaaaa agggcatact ccagacagtc aaagtagtgg atgagctagt
     4081 taaggtcatg ggacgtcaca aaccggaaaa cattgtaatc gagatggcac gcgaaaatca
     4141 aacgactcag aaggggcaaa aaaacagtcg agagcggatg aagagaatag aagagggtat
     4201 taaagaactg ggcagccaga tcttaaagga gcatcctgtg gaaaataccc aattgcagaa
     4261 cgagaaactt tacctctatt acctacaaaa tggaagggac atgtatgttg atcaggaact
     4321 ggacataaac cgtttatctg attacgacgt cgatgccatt gtaccccaat cctttttgaa
     4381 ggacgattca atcgacaata aagtgcttac acgctcggat aagaaccgag ggaaaagtga
     4441 caatgttcca agcgaggaag tcgtaaagaa aatgaagaac tattggcggc agctcctaaa
     4501 tgcgaaactg ataacgcaaa gaaagttcga taacttaact aaagctgaga ggggtggctt
     4561 gtctgaactt gacaaggccg gatttattaa acgtcagctc gtggaaaccc gccaaatcac
     4621 aaagcatgtt gcacagatac tagattcccg aatgaatacg aaatacgacg agaacgataa
     4681 gctgattcgg gaagtcaaag taatcacttt aaagtcaaaa ttggtgtcgg acttcagaaa
     4741 ggattttcaa ttctataaag ttagggagat aaataactac caccatgcgc acgacgctta
     4801 tcttaatgcc gtcgtaggga ccgcactcat taagaaatac ccgaagctag aaagtgagtt
     4861 tgtgtatggt gattacaaag tttatgacgt ccgtaagatg atcgcgaaaa gcgaacagga
     4921 gataggcaag gctacagcca aatacttctt ttattctaac attatgaatt tctttaagac
     4981 ggaaatcact ctggcaaacg gagagatacg caaacgacct ttaattgaaa ccaatgggga
     5041 gacaggtgaa atcgtatggg ataagggccg ggacttcgcg acggtgagaa aagttttgtc
     5101 catgccccaa gtcaacatag taaagaaaac tgaggtgcag accggagggt tttcaaagga
     5161 atcgattctt ccaaaaagga atagtgataa gctcatcgct cgtaaaaagg actgggaccc
     5221 gaaaaagtac ggtggcttcg atagccctac agttgcctat tctgtcctag tagtggcaaa
     5281 agttgagaag ggaaaatcca agaaactgaa gtcagtcaaa gaattattgg ggataacgat
     5341 tatggagcgc tcgtcttttg aaaagaaccc catcgacttc cttgaggcga aaggttacaa
     5401 ggaagtaaaa aaggatctca taattaaact accaaagtat agtctgtttg agttagaaaa
     5461 tggccgaaaa cggatgttgg ctagcgccgg agagcttcaa aaggggaacg aactcgcact
     5521 accgtctaaa tacgtgaatt tcctgtattt agcgtcccat tacgagaagt tgaaaggttc
     5581 acctgaagat aacgaacaga agcaactttt tgttgagcag cacaaacatt atctcgacga
     5641 aatcatagag caaatttcgg aattcagtaa gagagtcatc ctagctgatg ccaatctgga
     5701 caaagtatta agcgcataca acaagcacag ggataaaccc atacgtgagc aggcggaaaa
     5761 tattatccat ttgtttactc ttaccaacct cggcgctcca gccgcattca agtattttga
     5821 cacaacgata gatcgcaaac gatacacttc taccaaggag gtgctagacg cgacactgat
     5881 tcaccaatcc atcacgggat tatatgaaac tcggatagat ttgtcacagc ttgggggtga
     5941 ccccaagaag aagaggaaag tcaccggtca tcatcaccat caccattgag tttaaacccg
     6001 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     6061 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     6121 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     6181 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     6241 ttctgaggcg gaaagaacca gctggggctc gataccgtcg acctctagct agagcttggc
     6301 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6361 catacgagcc ggaagcataa agtgtaaagc ctagggtgcc taatgagtga gctaactcac
     6421 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6481 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6541 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6601 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6661 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6721 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6781 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6841 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6901 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6961 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7021 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7081 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7141 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7201 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7261 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7321 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7381 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7441 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7501 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7561 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7621 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7681 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     7741 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     7801 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     7861 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     7921 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     7981 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8041 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8101 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8161 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8221 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8281 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8341 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8401 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8461 tgacgtcgac ggatcgggag atcgatctcc cgatccccta gggtcgactc tcagtacaat
     8521 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     8581 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     8641 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     8701 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
     8761 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     8821 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     8881 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     8941 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     9001 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     9061 cgtattagtc atcgctatta ccatg
//
