LOCUS       sequence-306368-        9460 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of pegRNA(s) and sgRNA(s) under control of UAS promoter. 
            NS stands for ""No Scaffold"". Can be used to generate transgenic 
            flies with vermillion+ selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9460)
  TITLE     Prime editing expression plasmids for use in Drosophila melanogaster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCFD6-NS
FEATURES             Location/Qualifiers
     source          1..9460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    93..187
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    211..305
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    1301..1731
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    1765..1834
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(2053..2071)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2092..2108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2092..2108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2105..2127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2116..2132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2140..2146,2147..2164,2165..2170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2185..2206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2323..2340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2494..3082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2574..2593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3253..4044,4045..4113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3876..3895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4114..4218)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4244..4699)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4381..4402)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4593..4612
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4826..4848
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4840..4857
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4841..4857
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4864..4883
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4864..4882
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4916..4934)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5386..5457,5859..6132,6207..6861,6923..7238,7442..7573,
                     7644..8258)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    9031..9064
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    join(9077..9460,1..47)
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
ORIGIN
        1 tcaattgtgc tcggcaacag tatatttgtg gtgtgccaac caacaacaga tccaagctgg
       61 ccgcggctcg atccgcttgc atgcctgcag gtcggagtac tgtcctccga gcggagtact
      121 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc
      181 ctccgagcgg aagcttgcat gcctgcaggt cggagtactg tcctccgagc ggagtactgt
      241 cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct
      301 ccgagcggag actctagcga gctcgcccgg ggatcgagcg cagcggtata aaagggcgcg
      361 gggtggctga gagcatcagt tgtgaatgaa tgttcgagcc gagcagacgt gccgctgcct
      421 tcgttaatat cctttgaata agccaacttt gaatcacaag acgcatacca aacgaattcg
      481 ggctttgagt gtgtgtagac atcaagcatc ggtggttcag tggtagaatg ctcgcctgcc
      541 acgcgggcgg cccgggttcg attcccggcc gatgcagggt cttcgagaag acctaacaaa
      601 gcaccagtgg tctagtggta gaatagtacc ctgccacggt acagacccgg gttcgattcc
      661 cggctggtgc atctagagga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
      721 ggacaaacta cctacagaga tttaaagctc taattaaaat aacaaatcaa ttgttttata
      781 atattcgtac gattctttga ttatgtaata aaatgtgatc attaggaaga ttacgaaaaa
      841 tataaaaaat atgagttctg tgtgtataac aaatgctgta aacgccacaa ttgtgtttgt
      901 tgcaaataaa cccatgatta tttgattaaa attgttgttt tctttgttca tagacaatag
      961 tgtgttttgc ctaaacgtgt actgcataaa ctccatgcga gtgtatagcg agctagtggc
     1021 taacgcttgc cccaccaaag tagattcgtc aaaatcctca atttcatcac cctcctccaa
     1081 gtttaacatt tggccgtcgg aattaacttc taaagatgcc acataatcta ataaatgaaa
     1141 tagagattca aacgtggcgt catcgtccgt ttcgaccatt tccgaaaaga actcgggcat
     1201 aaactctatg atttctctgg acgtggtgtt gtcgaaactc tcaaagtacg cagtcaggaa
     1261 cgtgcgcgac atgtcgtcgg gaaactcgcg cggaaacatt tggccacgta ataagtgtgc
     1321 gttgaattta ttcgcaaaaa cattgcatat tttcggcaaa gtaaaatttt gttgcatacc
     1381 ttatcaaaaa ataagtgctg catacttttt agagaaacca aataattttt tattgcatac
     1441 ccgtttttaa taaaatacat tgcataccct cttttaataa aaaatattgc atactttgac
     1501 gaaacaaatt ttcgttgcat acccaataaa agattattat attgcatacc cgtttttaat
     1561 aaaatacatt gcataccctc ttttaataaa aaatattgca tacgttgacg aaacaaattt
     1621 tcgttgcata cccaataaaa gattattata ttgcatacct tttcttgcca taccatttag
     1681 ccgatcaatt gtgctcggca acagtatatt tgtggtgtgc caaccaacaa cggatccact
     1741 agtgtcgacg atgtaggtca cagtctcgaa gccgcggtgc gggtgccagg gcgtgccctt
     1801 gggctccccg ggcgcgtact ccacctcacc catctggtcc atcatgatga acgggtcgag
     1861 gtggcggtag ttgatcccgg cgaacgcgcg gcgcaccggg aagccctcgc cctcgaaacc
     1921 gctgggcgcg gtggtcacgg tgagcacggg acgtgcgacg gcgtcggcgg gtgcggatac
     1981 gcggggcagc gtcagcgggt tctcgacggt cacggcgggc atgtcgacac tagttctagc
     2041 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct
     2101 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2161 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     2221 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2281 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2341 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2401 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2461 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2521 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2581 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2641 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2701 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2761 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2821 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2881 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2941 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3001 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3061 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3121 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3181 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3241 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3301 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3361 accatctggc cccagtgctg caatgatacc gcgggaccca cgctcaccgg ctccagattt
     3421 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3481 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3541 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3601 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3661 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3721 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     3781 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     3841 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     3901 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     3961 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4021 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4081 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4141 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4201 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     4261 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     4321 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     4381 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     4441 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     4501 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     4561 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     4621 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     4681 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     4741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     4801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     4861 ttgtaatacg actcactata gggcgaattg ggtacgtacc gggcccctag tatgtatgta
     4921 agttaataaa acccattttt gcggaaagta gataaaaaaa acattttttt tttttactgc
     4981 actggatatc attgaactta tctgatcagt tttaaattta cttcgatcca agggtatttg
     5041 atgtaccagg ttctttcgat tacctctcac tcaaaatgac attccactca aagtcagcgc
     5101 tgtttgcctc cttctctgtc cacagaaata tcgccgtctc tttcgccgct gcgtccgcta
     5161 tctctttcgc caccgtttgt agcgttacgt agcgtcaatg tccgccttca gttgcatttt
     5221 gtcagcggtt tcgtgacgaa gctccaagcg gtttacgcca tcaattaaac acaaagtgct
     5281 gtgccaaaac tcctctcgct tcttattttt gtttgttttt tgagtgattg gggtggtgat
     5341 tggttttggg tgggtaagca ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat
     5401 caggagctat taattcgcgg aggcagcaaa cacccatctg ccgagcatct gaacaatgtg
     5461 agtagtacat gtgcatacat cttaagttca cttgatctat aggaactgcg attgcaacat
     5521 caaattgtct gcggcgtgag aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa
     5581 caaatagtga cacgaaacag attattctgg tagctgttct cgctatataa gacaattttt
     5641 gagatcatat catgatcaag acatctaaag gcattcattt tcgactatat tcttttttac
     5701 aaaaaatata acaaccagat attttaagct gatcctagat gcacaaaaaa taaataaaag
     5761 tataaaccta cttcgtagga tacttcgggg tactttttgt tcggggttag atgagcataa
     5821 cgcttgtagt tgatatttga gatcccctat cattgcaggg tgacagcgga gcggcttcgc
     5881 agagctgcat taaccagggc ttcgggcagg ccaaaaacta cggcacgctc cggccaccca
     5941 gtccgccgga ggactccggt tcagggagcg gccaactagc cgagaacctc acctatgcct
     6001 ggcacaatat ggacatcttt ggggcggtca atcagccggg ctccggatgg cggcagctgg
     6061 tcaaccggac acgcggacta ttctgcaacg agcgacacat accggcgccc aggaaacatt
     6121 tgctcaagaa cggtgagttt ctattcgcag tcggctgatc tgtgtgaaat cttaataaag
     6181 ggtccaatta ccaatttgaa actcagtttg cggcgtggcc tatccgggcg aacttttggc
     6241 cgtgatgggc agttccggtg ccggaaagac gaccctgctg aatgcccttg cctttcgatc
     6301 gccgcagggc atccaagtat cgccatccgg gatgcgactg ctcaatggcc aacctgtgga
     6361 cgccaaggag atgcaggcca ggtgcgccta tgtccagcag gatgacctct ttatcggctc
     6421 cctaacggcc agggaacacc tgattttcca agccatggtg cggatgccac gacatctgac
     6481 ctatcggcag cgagtggccc gcgtggatca ggtgatccag gagctttcgc tcagcaaatg
     6541 tcagcacacg atcatcggtg tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa
     6601 gcgtctggca ttcgcctccg aggctctaac cgatccgccg cttctgatct gcgatgagcc
     6661 cacctccgga ctggactcct ttaccgccca cagcgtcgtc caggtgctga agaagctgtc
     6721 gcagaagggc aagaccgtca tcctgaccat tcatcagccg tcatccgagc tgtttgagct
     6781 ctttgacaag atccttctga tggccgaggg cagggtagct ttcttgggca ctcccagcga
     6841 agccgtcgac ttcttttcct agtgagttcg atgtgtttat taagggtatc tagtattaca
     6901 taacatctca actcctatcc agcgtgggtg cccagtgtcc taccaactac aatccggcgg
     6961 acttttacgt acaggtgttg gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga
     7021 tcgccaagat atgcgacaat tttgccatta gcaaagtagc ccgggatatg gagcagttgt
     7081 tggccaccaa aaatctggag aagccactgg agcagccgga gaatgggtac acctacaagg
     7141 ccacctggtt catgcagttc cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg
     7201 aaccactcct cgtaaaagtg cgacttattc agacaacggt gagtggttcc agtggaaaca
     7261 aatgatataa cgcttacaat tcttggaaac aaattcgcta gattttagat agaattgcct
     7321 gattccacac ccttcttagt ttttttcaat gagatgtata gtttatagtt ttgcagaaga
     7381 taaataaatt tcatttaact cgcgaatatt aatgagatgc gagtaacatt ttaatttgca
     7441 gatggttgcc atcttgattg gcctcatctt tttgggccaa caactcacgc aagtgggtgt
     7501 gatgaatatc aacggagcca tcttcctctt cctgaccaac atgacctttc aaaacgtctt
     7561 tgccacgata aatgtaagtc atgtttagaa tacatttgca tttcaataat ttactaactt
     7621 tctaatgaat cgattcgatt taggtgttca cctcagagct gccagttttt atgagggagg
     7681 cccgaagtcg actttatcgc tgtgacacat actttctggg caaaacgatt gccgaattgc
     7741 cgctttttct cacagtgcca ctggttttca cggcgattgc ctatccgatg atcggactgc
     7801 gggccggagt gctgcacttc ttcaactgcc tggcgctggt cactctggtg gccaatgtgt
     7861 caacgtcctt cggatatcta atatcctgcg ccagctcctc gacctcgatg gcgctgtctg
     7921 tgggtccgcc ggttatcata ccattcctgc tctttggcgg cttcttcttg aactcgggct
     7981 cggtgccagt atacctcaaa tggttgtcgt acctctcatg gttccgttac gccaacgagg
     8041 gtctgctgat taaccaatgg gcggacgtgg agccgggcga aattagctgc acatcgtcga
     8101 acaccacgtg ccccagttcg ggcaaggtca tcctggagac gcttaacttc tccgccgccg
     8161 atctgccgct ggactacgtg ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat
     8221 atctggctct aagacttcgg gcccgacgca aggagtagcc gacatatatc cgaaataact
     8281 gcttgttttt ttttttacca ttattaccat cgtgtttact gtttattgcc ccctcaaaaa
     8341 gctaatgtaa ttatatttgt gccaataaaa acaagatatg acctatagaa tacaagtatt
     8401 tccccttcga acatccccac aagtagactt tggatttgtt tctaaccaaa agacttacac
     8461 acctgcatac cttacatcaa aaactcgttt atcgctacat aaaacaccgg gatatatttt
     8521 ttatatacat acttttcaaa tcgcgcgccc tcttcataat tcacctccac cacaccacgt
     8581 ttcgtagttg ctctttcgct gtctcccacc cgctctccgc aacacattca ccttttgttc
     8641 gacgaccttg gagcgactgt cgttagttcc gcgcgattcg gttcgctcaa atggttccga
     8701 gtggttcatt tcgtctcaat agaaattagt aataaatatt tgtatgtaca atttatttgc
     8761 tccaatatat ttgtatatat ttccctcaca gctatattta ttctaattta atattatgac
     8821 tttttaaggt aattttttgt gacctgttcg gagtgattag cgttacaatt tgaactgaaa
     8881 gtgacatcca gtgtttgttc cttgtgtaga tgcatctcaa aaaaatggtg ggcataatag
     8941 tgttgtttat atatatcaaa aataacaact ataataataa gaatacattt aatttagaaa
     9001 atgcttggat ttcactggaa ctaggctagc ataacttcgt ataatgtatg ctatacgaag
     9061 ttatgctagc ggatcctggc cacgtaataa gtgtgcgttg aatttattcg caaaaacatt
     9121 gcatattttc ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata
     9181 ctttttagag aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca
     9241 taccctcttt taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc
     9301 aataaaagat tattatattg catacccgtt tttaataaaa tacattgcat accctctttt
     9361 aataaaaaat attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt
     9421 attatattgc ataccttttc ttgccatacc atttagccga
//
